Article
Biochemistry & Molecular Biology
Mojtaba Mohammadi Arani, Amir Parviz Salati, Saeed Keyvanshokooh, Omid Safari
Summary: The study found that supplementing zebrafish with 4 x 10^6 CFU PA g(-1) improved their growth and reproductive performance, as well as enhancing mucosal immune responses. Additionally, this concentration of PA also impacted alkaline phosphatase activity in skin mucus, intestinal villus length, and the expression of the Cyp19a gene.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Environmental Sciences
Liyuan Qiang, Jinping Cheng
Summary: This study found that exposure to polystyrene microplastics can induce molecular responses and histological alterations in fish gonads, potentially causing adverse effects on fish reproductive organs.
Article
Environmental Sciences
Ming Jia, Miaomiao Teng, Sinuo Tian, Jin Yan, Zhiyuan Meng, Sen Yan, Ruisheng Li, Zhiqiang Zhou, Wentao Zhu
Summary: The study found that long-term low-dose exposure to PEN did not significantly affect the growth and spawning of zebrafish, but led to significant changes in important hormones and related gene expressions in zebrafish.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Fisheries
Alice Printzi, Stefanos Fragkoulis, Anastasia Dimitriadi, Kleoniki Keklikoglou, Christos Arvanitidis, P. Eckhard Witten, George Koumoundouros
Summary: Exercise in zebrafish has been shown to significantly increase the occurrence of lordosis in the vertebral column. Through various methods of examination, abnormal vertebrae with dorsal folding were observed in the exercise group. This study provides insights into mechanisms that trigger lordotic spine malformations in vertebrates.
JOURNAL OF FISH BIOLOGY
(2021)
Article
Food Science & Technology
Chao Wang, Lei Gao, Yansong Gao, Ge Yang, Zijian Zhao, Yujuan Zhao, Jihui Wang, Shengyu Li
Summary: A novel probiotic strain Pediococcus acidilactici AS185, isolated from traditional Chinese fermented foods, was used as an adjunct culture for probiotic cheddar cheese production. The addition of P. acidilactici AS185 did not affect the physicochemical composition of cheddar cheese, but improved its flavor and taste. The strain was able to grow during ripening and promote the generation of beneficial compounds, and was protected by the cheese matrix during simulated gastrointestinal transit.
FOOD SCIENCE & NUTRITION
(2023)
Article
Biotechnology & Applied Microbiology
Mengdi Zhao, Yuanyuan Zhang, Yueyao Li, Keyuan Liu, Chao Zhang, Guangyu Li
Summary: Pediococcus acidilactici CLP03, isolated from Felis catus, exhibited inhibitory properties against pathogenic bacteria, survived in gastrointestinal conditions, and demonstrated good oxidation resistance. Animal experiments showed that gavage of CLP03 had no toxic effects on mice and enhanced their antioxidant capacity.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2023)
Article
Microbiology
Mengdi Zhao, Keyuan Liu, Yuanyuan Zhang, Yueyao Li, Ning Zhou, Guangyu Li
Summary: This study reports the probiotic properties, safety, and whole-genome sequence of Pediococcus acidilactici GLP06 isolated from beagles' feces for the first time. P. acidilactici GLP06 showed better probiotic potential and was non-toxic to mice. The whole-genome sequencing revealed no drug resistance genes in P. acidilactici GLP06.
FRONTIERS IN MICROBIOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Mathieu Castex, Eric Leclercq, Pierrette Lemaire, Liet Chim
Summary: This study evaluated the effect of supplementation with the single-strain probiotic Pedicococcus acidilactici MA18/5M on the growth, nutritional indices, and metabolic status of adult western blue shrimp, Litopenaeus stylirostris. The results showed that the probiotic improved feed conversion efficiency, daily growth rate, and antioxidant status, suggesting promising prospects for optimizing shrimp feed formulation and industry performance.
Article
Multidisciplinary Sciences
Pawiya Pupa, Prasert Apiwatsiri, Wandee Sirichokchatchawan, Nopadon Pirarat, Teerawut Nedumpun, David J. Hampson, Nongnuj Muangsin, Nuvee Prapasarakul
Summary: This study investigated the effects of microencapsulated probiotics on weaned pigs infected with enterotoxigenic Escherichia coli (ETEC). The results showed that microencapsulated probiotics improved the growth performance, gut microbiota balance, and immune status of weaned pigs. Additionally, probiotic supplementation reduced inflammation and intestinal damage.
SCIENTIFIC REPORTS
(2022)
Article
Agriculture, Multidisciplinary
Hyunwoo Ahn, Geonhak Lee, Woorin Lee, Mingyu Kim, Kwang-Geun Lee
Summary: This study evaluated the probiotic abilities of bacteriocin-producing LAB isolated from malted barley, Pediococcus acidilactici HW01, and Leuconostoc citreum HW02. The results showed that both strains could survive in harsh GI conditions and exhibited good adhesion and antimicrobial activity. They also demonstrated anti-inflammatory effects. Therefore, they may be good probiotic candidates.
CHEMICAL AND BIOLOGICAL TECHNOLOGIES IN AGRICULTURE
(2023)
Article
Fisheries
Xiaoteng Fan, Ling Cui, Tingting Hou, Xue Xue, Shuai Zhang, Zaizhao Wang
Summary: The study showed that starvation stress significantly impacted the organismal development, spermatogenesis, testicular inflammation, and apoptotic activities in male zebrafish, with decreased biological indexes, impaired testicular architecture, elevated immune response, and stimulated apoptotic activity. The findings highlighted the susceptibility of fish gonads to food fluctuation.
DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Sandeep Jaiswal, Satya Narayan Pradhan, Diamond Jain, Magesh Peter Dhassiah Peter, Usha Antony
Summary: This study identified Pediococcus acidilactici UAMS, a strain isolated from the traditional fermented rice beverage Bhaati jaanr, as a potential probiotic with bioprotective abilities. The isolated strain exhibited high butyrate production, tolerance to gastric and bile stress, and showed potential therapeutic effects against colon cancer cells HT29 and SW480.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Microbiology
Komwit Surachat, Duangporn Kantachote, Panchalika Deachamag, Monwadee Wonglapsuwan
Summary: This study characterized the molecular properties of Pediococcus acidilactici HN9 and compared its genome with other bacterial strains in the genus Pediococcus. Various bioinformatic approaches were used to investigate phylogenetic relationships, genome stability, virulence factors, bacteriocin production, and antimicrobial resistance genes of the HN9 strain. The study provided insights into the adaptability of HN9 to different environments and its mechanism for maintaining genome stability.
Article
Chemistry, Multidisciplinary
Hafsa Kanwal, Alessandro Di Cerbo, Freeha Zulfiqar, Carla Sabia, Amara Nawaz, Fariha Masood Siddiqui, Muhammad Aqeel, Shakira Ghazanfar
Summary: This study demonstrates the beneficial effects of Pediococcus acidilactici isolated from the gut of Nili Ravi water buffalo, showing high enzymatic potential, antimicrobial activity, and survivability at a wide pH range. The relatively low genetic diversity of the isolated strain suggests its stability for potential use as a starter culture and feed probiotic in the dairy industry. Further research is needed to determine the optimal dosage, time frame, and intake frequency of this probiotic strain.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Xujia Zhang, Emma Ivantsova, Veronica Perez-Rodriguez, Fangjie Cao, Christopher L. Souders II, Christopher J. Martyniuk
Summary: This study investigates the effects of dinoseb on zebrafish during early stages of development. The results show that dinoseb impairs mitochondrial oxidative phosphorylation, alters immune system responses, and affects locomotor activity in fish.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY
(2022)
Article
Fisheries
Ane del Rio Lavin, Natalia Diaz-Arce, Maria Angelica Larrain, Cristian Araneda, Naiara Rodriguez-Ezpeleta, Elisa Jimenez, Miguel Angel Pardo
Summary: Seafood traceability is important for regulators and fishing industries to prevent fraud and ensure consumer safety. This study used genetic markers to trace the genetic structure of Mediterranean mussel and assess the ability of new markers to trace the origin of samples. The results showed clear differentiation between populations from different regions and suggested the potential for developing an accurate and cost-effective traceability tool.
Article
Multidisciplinary Sciences
Mohamed A. A. Hassan, Ramy K. A. Sayed, Mohammed Abdelsabour-Khalaf, Enas A. Abd-Elhafez, L. Anel-Lopez, M. F. Riesco, C. Ortega-Ferrusola, R. Montes-Garrido, M. Neila-Montero, L. Anel, M. Alvarez
Summary: The study found significant variations in blood flow velocity and vascular resistance in the supratesticular artery regions, and histological and morphometric measurements also showed differences among different regions.
SCIENTIFIC REPORTS
(2022)
Article
Reproductive Biology
Marta Neila-Montero, Marta F. Riesco, Rafael Montes-Garrido, Cristina Palacin-Martinez, Cesar Chamorro, Paulino de Paz, Mercedes Alvarez, Luis Anel, Luis Anel-Lopez
Summary: The study aimed to define the optimal conditions for centrifugation of ram semen in order to ensure the unaffected fertilization ability of the sperm. The results showed that centrifugation at 1200xg for 10 minutes using INRA 96 extender, slow refrigeration, and room temperature achieved high recovery rates and good quality sperm without detrimental effects on fertility.
Article
Agriculture, Dairy & Animal Science
Cristina Palacin-Martinez, Mercedes Alvarez, Rafael Montes-Garrido, Marta Neila-Montero, Luis Anel-Lopez, Paulino de Paz, Luis Anel, Marta F. Riesco
Summary: Sperm cryopreservation plays a crucial role in optimizing artificial insemination protocols for rams. Previous efforts to improve ram sperm cryopreservation protocols have focused on different methods, such as using different cryoprotectants, antioxidants, or freezing-thawing rates. However, the influence of male sexual regimes on freezeability has not been studied until now. In this study, a multiparametric analysis was conducted to evaluate the quality of cooled and frozen-thawed semen after different sperm collection frequencies. The results showed that semen quality significantly decreased in the groups with zero and ten collections per week compared to the group with four collections per week. Additionally, the apoptosis rate significantly increased in the group with ten collections per week. Enzyme activity related to redox balance, such as glutathione peroxidase and superoxide dismutase, also showed significant decreases in the group with ten collections per week. This study highlights the importance of considering male sexual regimes in alternative strategies for sperm cryopreservation in order to improve frozen-thawed sperm quality.
Article
Veterinary Sciences
Rafael Montes-Garrido, Marta F. Riesco, Luis Anel-Lopez, Marta Neila-Montero, Cristina Palacin-Martinez, Juan Carlos Boixo, Paulino de Paz, Cristina Ortega-Ferrusola, Mohamed A. A. Hassan, Luis Anel, Mercedes Alvarez
Summary: The frequency of semen collection is an important factor affecting the performance of rams in breeding centers. Ultrasound technology can be used to evaluate and predict changes in sperm quality. The study found that increasing the frequency of semen collection led to an increase in testicular echotexture and doppler parameters. Sperm motility and functionality decreased with increased frequency, indicating the impact of collection frequency on sperm quality. Ultrasound technology can be used as a predictive tool for assessing the effects of semen collection frequency on sperm quality in donor rams.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biology
David G. Valcarce, Marta F. Riesco, Leyre Cuesta-Martin, Anna Esteve-Codina, Juan Manuel Martinez-Vazquez, Vanesa Robles
Summary: Chronic stress has negative effects on behavior, reproductive parameters, and progeny in zebrafish. It induces anxiety-like behavior, overexpression of endoplasmic reticulum stress-related genes in the brain, and compromised sperm motility. Additionally, chronic stress alters the molecular status and development of progeny.
Correction
Reproductive Biology
Marta Neila-Montero, Marta F. Riesco, Rafael Montes-Garrido, Cristina Palacin-Martinez, Cesar Chamorro, Paulino de Paz, Mercedes Alvarez, Luis Anel, Luis Anel-Lopez
Article
Biochemistry & Molecular Biology
David G. Valcarce, Marta F. Riesco, Juan Manuel Martinez-Vazquez, Jose Luis Rodriguez Villanueva, Vanesa Robles
Summary: This experiment aimed to evaluate the potential links between handling during culture with stress responses and growth on Senegalese sole. The results showed that reducing handling-derived stressors led to lower levels of circulating cortisol in the fish and promoted higher growth. The gene expression study further supported the findings of this experiment.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2023)
Article
Food Science & Technology
Ana Isabel Puertas, Ma Goretti Llamas-Arriba, Oier Etxebeste, Inaki Berregi, Miguel Angel Pardo, Alicia Prieto, Paloma Lopez, Teresa Duenas
Summary: This study characterizes the physico-chemical properties of heteropolysaccharides (HePS) produced by Liquorilactobacillus sicerae CUPV261T and Secundilactobacillus collinoides CUPV237 strains isolated from cider. Genome sequencing revealed the presence of at least four putative HePS gene clusters in each strain, which correlated with their ability to secrete HePS. The HePS were resistant to gastric stress conditions and showed significant reduction in zebrafish larvae mortality in an in vivo model of inflammatory bowel disease.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2023)
Article
Reproductive Biology
Marta Neila-Montero, Mercedes Alvarez, Marta F. Riesco, Rafael Montes-Garrido, Cristina Palacin-Martinez, Antonio Silva-Rodriguez, Francisco E. Martin-Cano, Fernando J. Pena, Paulino de Paz, Luis Anel, Luis Anel-Lopez
Summary: This study evaluated fertility rates after cervical artificial insemination of 11,805 ewes at different points in the reproductive season and identified male factors influencing reproductive success. Proteomic analysis revealed different sperm protein expression between the extreme points in the mating season, potentially explaining the lower fertility rate during early breeding season. Sperm proteins can be used as effective molecular markers in predicting fertilization ability related to seasonal variations.
Article
Agriculture, Dairy & Animal Science
Rafael Montes-Garrido, Luis Anel-Lopez, Marta F. Riesco, Marta Neila-Montero, Cristina Palacin-Martinez, Cristina Soriano-Ubeda, Juan Carlos Boixo, Paulino de Paz, Luis Anel, Mercedes Alvarez
Summary: This study aimed to determine the most suitable tool and formula for calculating testicular volume under field conditions to improve the accurate prediction of sperm production in rams. The use of ultrasonography and a new formula adapted to rams showed promise in enhancing the prediction of sperm production.
Article
Fisheries
Simrith E. Cordova-de la Cruz, Marta F. Riesco, Gil Martinez-Bautista, Daniel Calzada-Ruiz, Talhia Martinez-Burguete, Emyr S. Pena-Marin, Carlos Alfonso Alvarez-Gonzalez, Ignacio Fernandez
Summary: This study examines the effects of temperature on development, hatching, and growth in tropical gar fish, finding that higher temperatures lead to early hatching and advanced skeletal development. Additionally, fish larvae raised under different temperature regimes exhibit distinct body morphologies and ecological characteristics, highlighting the importance of understanding the impacts of global warming on early fish development.
