Journal
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
Volume 13, Issue 9, Pages 4453-4458Publisher
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
DOI: 10.7314/APJCP.2012.13.9.4453
Keywords
Tanshinone II-A; colorectal carcinoma; angiogenesis; cyclooxygenase 2; vascular endothelial growth factor
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Funding
- Science and Technology Commission of Shanghai Municipality [10ZR1427400, 09ZR1428500, 114119b3100, 11nm0504500]
- Program of Shanghai Municipal Education Commission [12YZ058]
- Program of Shanghai Municipal Health Bureau [2010161, 2011ZJ030]
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Angiogenesis plays a significant role in colorectal cancer (CRC) and cyclooxygenase-2 (COX-2) appears to be involved with multiple aspects of CRC angiogenesis. Our aim was to investigate the inhibitory effects of Tan II-A (Tanshinone II-A, Tan II-A) on tumor growth in mice, as well as alteration of expression of COX-2 and VEGF in CRC. We established the mice xenograft model of C26 CRC cell line, and injected 0.5, 1, 2mg/kg of Tan II-A and 1mg/kg of 5-FU in respectively in vivo. Then, we assayed tumor weight and volume, and evaluated microvascular density and expression of VEGF. COX-2 promoter and COX-2 plasmids were transfected into HCT-116 cells, followed by detection of COX-2 promoter activity by chemiluminescence, and detection of COX-2 mRNA expression by fluorescence quantitative PCR. Taken together, the results showed Tan II-A could inhibit tumor growth and suppress the VEGF level in vivo. HCT-116 cell experiments showed marked inhibitory effects of Tan II-A on COX-2 and VEGF in a dose-dependent manner. The results indicate that Tan II-A can effectively inhibit tumor growth and angiogenesis of human colorectal cancer via inhibiting the expression level of COX-2 and VEGF.
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