Journal
ASIAN PACIFIC JOURNAL OF CANCER PREVENTION
Volume 13, Issue 2, Pages 653-664Publisher
ASIAN PACIFIC ORGANIZATION CANCER PREVENTION
DOI: 10.7314/APJCP.2012.13.2.653
Keywords
E. foetidum; iNOS; COX 2; TNF alpha; IL 6; inflammatory mediators
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Funding
- National Science and Technology Development Agency, Thailand
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Objective: This study assessed anti-inflammatory and antioxidant activities of E. foetidum leaf extract on LPS-activated murine macrophages. Methods: RAW264.7 cells were pretreated with or without E. foetidum extract for 1 h prior to incubation with LPS for 24 h. Anti-inflammatory activity was evaluated with reference to iNOS, COX-2, TNF-alpha and IL-6 gene expression. In addition, NO and intracellular ROS generation were determined by Griess method and fluorescence intensity and activation of MAPKs and I kappa B by Western blotting. Results: Prior treatment with E. foetidum leaf extract inhibited elevation of IL-6, TNF-alpha, iNOS and COX-2, together with their cognate mRNAs in a dose-dependent manner. NO and intracellular ROS contents were similarly reduced. These effects were due to inhibition of LPS-induced phosphorylation of JNK and p38 as well as I kappa B. E. foetidum ethanol extract were shown to contain lutein, beta-carotene, chlorogenic acid, kaempferol and caffeic acid, compounds known to exert these bioactive properties. Conclusions: E. foetidum leaf extract possesses suppressive effects against pro-inflammatory mediators. Thus, E. foetidum has a high potential to be used as a food supplement to reduce risk of cancer associated with inflammation.
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