Article
Biochemistry & Molecular Biology
Malgorzata Grzesiak, Kinga Kaminska, Aleksandra Bodzioch, Ewa M. Drzewiecka, Anita Franczak, Katarzyna Knapczyk-Stwora
Summary: This study reveals the presence of vitamin D-3 metabolism in the uterus of pigs, with both endometrium and myometrium capable of synthesizing vitamin D-3, while only the endometrium is involved in its metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Endocrinology & Metabolism
Jie Yu, Sarah L. Berga, Qingying Meng, Mingjing Xia, Trudy A. Kohout, Marcel van Duin, Robert N. Taylor
Summary: The study demonstrated the important regulatory role of dopamine receptor type 2 in endometrial tissue and cells, showing that dopamine agonists like cabergoline can promote decidualization in vitro, potentially serving as therapeutic agents for infertility due to inflammation.
JOURNAL OF CLINICAL ENDOCRINOLOGY & METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Barbara Jana, Jaroslaw Calka, Bartosz Micinski
Summary: Inflammation reduces GALR1 protein expression in pig myometrium, while GALR1 and GALR2 participate in the contractile regulation of an inflamed uterus.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Hwanhee Jang, Soohyung Lee, Inkyu Yoo, Yohan Choi, Jisoo Han, Yugyeong Cheon, Hakhyun Ka
Summary: S100A8, S100A9, and S100A12, calcium-binding proteins, are dynamically expressed in response to conceptus-derived signals at the maternal-conceptus interface, potentially playing a critical role in regulating endometrial epithelial cell function and conceptus implantation to support the establishment and maintenance of pregnancy in pigs.
BIOLOGY OF REPRODUCTION
(2022)
Article
Cell Biology
Damian Tanski, Agnieszka Skowronska, Malgorzata Tanska, Ewa Lepiarczyk, Mariusz T. Skowronski
Summary: The research shows that estradiol, progesterone, and arachidonic acid regulate the expression of AQP1 and AQP5 in porcine luminal epithelial cells through the PKA and MAPK signaling pathways.
Article
Reproductive Biology
Martyna M. Maopolska, Ryszard Tuz, Tomasz Schwarz, Lochi-Don Ekanayake, Jessica D'Ambrosio, Bahareh Ahmadi, Jacek Nowicki, Ewa Tomaszewska, Magorzata Grzesiak, Pawel M. Bartlewski
Summary: This study identifies several anatomical and histomorphological metrics related to the reproductive performance of swine, which can inform production protocols and aid in the selection of elite breeding sows. Further research is warranted into non-invasive or minimally invasive techniques for obtaining such measures.
Article
Cell Biology
Kinga Orzechowska, Kamil Dobrzyn, Marta Kiezun, Agata Malinowska, Bianka Swiderska, Tadeusz Kaminski, Nina Smolinska
Summary: This study examined the effects of CHEM on differentially regulated proteins in porcine endometrial tissue during implantation. It was found that CHEM modulates protein expressions involved in endometrial cell growth, migration, immune response, sensitivity to P4, and formation of new blood vessels. CHEM also appears to play a role in endothelial cell dysfunction during preeclampsia.
Article
Agriculture, Dairy & Animal Science
Kinga Orzechowska, Marta Kiezun, Edyta Rytelewska, Marlena Gudelska, Katarzyna Kisielewska, Grzegorz Kopij, Kamil Dobrzyn, Ewa Zaobidna, Karolina Szymanska, Tadeusz Kaminski, Nina Smolinska
Summary: This study aimed to evaluate the effects of orexin A (OXA) on mRNA abundance of enzymes involved in prostaglandin production and the concentrations of prostaglandin E2 (PGE2) and F-2 alpha (PGF(2 alpha)) in endometrial and myometrial explants. The results suggest that OXA may affect the synthesis and secretion of PGE2 and PGF(2 alpha) in the uterus by regulating the mRNA levels of enzymes involved in prostaglandin production.
ANIMAL REPRODUCTION SCIENCE
(2022)
Article
Rheumatology
Cecilia Ansalone, John Cole, Sabarinadh Chilaka, Flavia Sunzini, Shatakshi Sood, Jamie Robertson, Stefan Siebert, Iain B. McInnes, Carl S. Goodyear
Summary: The study found that TNF plays a critical regulatory role in the differentiation of CD14(+) monocytes into osteoclasts, while a specific CD14(-)CD16(-)CD11c(+) pre-osteoclast population is exempt from this negative regulation. In healthy individuals, TNF drives epigenetic modification of the RANK gene via a specific pathway and inhibits osteoclastogenesis; whereas in patients with rheumatoid arthritis, altered epigenetic states in CD14(+) monocytes result in dysregulated TNF-mediated osteoclast homeostasis.
ANNALS OF THE RHEUMATIC DISEASES
(2021)
Article
Chemistry, Applied
Xinhua Hou, Run Zhang, Man Yang, Naiqi Niu, Junchao Wu, Ze Shu, Pengfei Zhang, Lijun Shi, Fuping Zhao, Ligang Wang, Lixian Wang, Longchao Zhang
Summary: Chinese indigenous pig breeds have higher intramuscular fat content and better meat quality. This study investigated the differential metabolites and lipids in the skeletal muscle of Laiwu and Yorkshire pigs, revealing their association with intramuscular fat content and meat flavor. The results showed that the Laiwu pig had a faster lipid droplet formation and contained more triglyceride, reflecting its higher intramuscular fat content.
Article
Agriculture, Dairy & Animal Science
Qiuyu Jiang, Jingjing Sun, Yang He, Yanbo Ma, Bingkun Zhang, Yanming Han, Yuanyuan Wu
Summary: The study showed that dietary hydroxychloride trace elements supplementation improved egg quality in aged hens by enhancing histological structure and immune responses of the uterus, compared with sulphate sources of zinc, copper, and manganese.
Article
Biochemistry & Molecular Biology
Ewa Tomaszewska, Malgorzata Swiatkiewicz, Siemowit Muszynski, Janine Donaldson, Katarzyna Ropka-Molik, Marcin B. Arciszewski, Maciej Murawski, Tomasz Schwarz, Piotr Dobrowolski, Sylwia Szymanczyk, Slawomir Dresler, Joanna Bonior
Summary: Chronic pancreatitis (CP) is an irreversible and progressive inflammatory disease. This study aimed to investigate the changes in pro-inflammatory cytokines, antioxidant defense system, and selected serum biochemical parameters in a cerulein-induced CP model in pigs. The study found that repetitive cerulein injections not only led to CP and induced inflammation in the intestine, but also impaired the intestinal barrier.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Zixi Chen, Jinpeng Li, Wenjuan Xu, Xiaomei Wu, Fenfen Xiang, Xiaoxiao Li, Mengzhe Zhang, Jin Zheng, Xiangdong Kang, Rong Wu
Summary: The study found that TLR4 expression on CD14+ maternal blood monocytes was significantly higher in the third trimester compared to the first and second trimesters. Maternal serum concentrations of TLR4 and cytokines were significantly higher in the term labor group compared to the term non-labor group.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Chemistry, Medicinal
Salvatore Pacifico, Valentina Albanese, Davide Illuminati, Erika Marzola, Martina Fabbri, Federica Ferrari, Victor A. D. Holanda, Chiara Sturaro, Davide Malfacini, Chiara Ruzza, Claudio Trapella, Delia Preti, Ettore Lo Cascio, Alessandro Arcovito, Stefano Della Longa, Martina Marangoni, Davide Fattori, Romina Nassini, Girolamo Calo, Remo Guerrini
Summary: In this study, 31 peptides were synthesized and tested for their activity at human recombinant NOP/opioid receptors. The peptide [Dmt(1,5)]N/OFQ(1-13)-NH2 was identified as the most potent dual NOP/mu receptor agonist, with antitussive effects demonstrated in vivo. The molecular mechanisms of peptide binding to the mu receptor were elucidated through experimental and in silico studies.
JOURNAL OF MEDICINAL CHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Kamil Dobrzyn, Marta Kiezun, Karol Szeszko, Katarzyna Kisielewska, Edyta Rytelewska, Marlena Gudelska, Ewa Zaobidna, Kinga Bors, Grzegorz Kopij, Karolina Szymanska, Barbara Kaminska, Tadeusz Kaminski, Nina Smolinska
Summary: This study found that orexin B influences the expression of a large number of genes in the porcine endometrial transcriptome, affecting processes crucial for reproductive success. Orexin B plays a bivalent role in the early-pregnant uterus, impacting pregnancy outcome, embryo development, immune system, etc.
REPRODUCTION IN DOMESTIC ANIMALS
(2021)
Article
Agriculture, Dairy & Animal Science
Anita Franczak, Ewa M. Waszkiewicz, Wiktoria Kozlowska, Agata Zmijewska, Anna Koziorowska
ANIMAL REPRODUCTION SCIENCE
(2020)
Article
Agriculture, Dairy & Animal Science
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Waszkiewicz, Alina Gajewska, Stanislaw Okrasa, Anita Franczak
REPRODUCTION IN DOMESTIC ANIMALS
(2020)
Article
Agriculture, Dairy & Animal Science
Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anna Koziorowska, Anita Franczak
Summary: This study investigated the effects of EMF on androgen synthesis and release from pig endometrium, finding that EMF may have suppressive effects on endometrial testosterone release and increased androstenedione release. Progesterone did not protect the pig endometrium against EMF radiation during the fetal peri-implantation period.
ANIMAL REPRODUCTION SCIENCE
(2021)
Article
Reproductive Biology
Justyna Kolakowska, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Serhiy Souchelnytskyi, Anita Franczak
Summary: The study aimed to find unique proteins in porcine conceptuses specifically during the peri-implantation period, leading to the discovery of 24 unique proteins involved in processes like adhesion, cadherin, and actin filament organization. These unique proteins may play a role in embryo development and acquisition of implantation competence.
REPRODUCTIVE BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anita Franczak
Summary: Electromagnetic fields can alter the expression levels of CYP19A3 and HSD17B4 in pig myometrium during the peri-implantation period, while progesterone may mitigate the effects of EMF, regulating the expression levels of aromatase.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Reproductive Biology
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Drzewiecka, Anita Franczak
Summary: KISS and RFRP-3 systems are present in the pituitary of estrous-cyclic and pregnant pigs, potentially affecting the synthesis and secretion of FSH by pituitary cells.
Article
Biochemistry & Molecular Biology
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Lukasz Paukszto, Agata Zmijewska, Pawel Jozef Wydorski, Jan Pawel Jastrzebski, Anita Franczak
Summary: The study found that EMF treatment affects gene expression in the myometrium of pigs during the peri-implantation period, mainly involving defense and immune responses. Analysis revealed that DEGs enrich multiple signaling pathways, and also uncovered the regulatory mechanisms of EMF through long non-coding RNAs and single nucleotide variants on the transcriptomic profile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Multidisciplinary Sciences
Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Anita Franczak
Summary: This study revealed that dietary restriction during the peri-conceptional period causes changes in gene expression in the pig myometrium, particularly those involved in endocrine activity, embryo-maternal interactions, and the regulation of cell cycle and proliferation.
SCIENTIFIC REPORTS
(2021)
Article
Reproductive Biology
Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anita Franczak
Summary: The study aims to investigate the effects of EMF exposure on estrogen synthesis and release in the endometrium. The results suggest that EMF exposure affects gene expression and is related to P-4 treatment. Additionally, EMF disrupts the synthesis and release of E-1 and E-2 in the endometrium.
REPRODUCTIVE BIOLOGY
(2022)
Article
Reproductive Biology
Agata Zmijewska, Wioleta Czelejewska, Ewa M. Drzewiecka, Anita Franczak
Summary: The pituitary gland plays a crucial role in female reproduction. However, little is known about the changes in the transcriptome of the porcine pituitary during reproductive status. This study aimed to analyze and compare the transcriptome profile of the pituitary gland in pregnant pigs and pigs during the estrous cycle. The analysis revealed 482 differentially expressed genes, with 68 up-regulated and 414 down-regulated in pregnant pigs. These findings provide insights into the molecular mechanisms involved in maternal recognition of pregnancy in pigs.
Article
Reproductive Biology
Anita Franczak, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski
Summary: Previous research has shown that the ELF-EMF can induce molecular alterations in the porcine myometrium. In this study, it was found that exposure to ELF-EMF increased DNMT1 expression, decreased DNMT3a expression, and increased genomic DNA methylation level in porcine myometrium. Moreover, the amplification level of methylated and unmethylated variants of the promoter region of selected genes was also affected by ELF-EMF radiation.
Article
Developmental Biology
Pawel Jozef Wydorski, Wiktoria Kozlowska, Ewa Monika Drzewiecka, Agata Zmijewska, Anita Franczak
Summary: Short-term ELF-EMF treatment may alter the DNA methylation level of genes in the endometrium. The changes in DNA methylation induced by ELF-EMF may affect the transcriptomic profile of the endometrium and disrupt physiological processes such as implantation and embryo development.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2023)
Article
Physiology
M. Martyniak, E. M. Drzewiecka, A. Zmijewska, A. Franczak, G. Kotwica
Summary: This study found that the gene expression in the epithelial cells of the ampulla and isthmus of the oviducts in pigs was altered during the peri-conceptional period, possibly due to DNA methylation. By evaluating important genes related to embryo development and determining the level of DNA methylation, it was found that the methylation levels of certain genes have changed. This suggests that the presence of embryos in the oviduct can change the transcriptomic profile and DNA methylation level in the epithelial cells.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2023)
Article
Developmental Biology
Anita Franczak, Ewa Monika Drzewiecka, Wiktoria Kozlowska, Agata Zmijewska, Pawel Jozef Wydorski, Anna Koziorowska
Summary: This study demonstrates that electromagnetic field radiation affects the steroidogenesis and release from pig conceptuses during the pre-implantation period. It also suggests that progesterone may have a protective effect against the effects of electromagnetic field radiation.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2022)
Article
Physiology
W. Kozlowska, E. M. Drzewiecka, L. Paukszto, A. Zmijewska, P. J. Wydorski, J. P. Jastrzebski, A. Franczak
Summary: This study found that exposure to low-frequency electromagnetic field alters the gene expression profile of pig endometrium, specifically affecting genes encoding proteins involved in proliferation and metabolism.
JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY
(2021)