Article
Biochemistry & Molecular Biology
Hitomi Ohinata, Takashi Obama, Tomohiko Makiyama, Yuichi Watanabe, Hiroyuki Itabe
Summary: This study found that high-density lipoproteins (HDL) partially decreased neutrophil extracellular trap (NET) formation induced by copper-oxidized low-density lipoprotein (oxLDL). It was also discovered that oxidized phosphatidylcholines and lysophosphatidylcholine in oxLDL promoted NET formation, which could be completely blocked by native HDL. Furthermore, an electronegative subfraction of LDL, LDL(-), was found to promote NET formation. These findings suggest that plasma lipoproteins and their oxidative modifications play multiple roles in promoting NET formation, and HDL acts as a suppressor of this response.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Simon Kraler, Florian A. Wenzl, Jody Vykoukal, Johannes F. Fahrmann, Ming-Yi Shen, Der-Yuan Chen, Kuan-Cheng Chang, Ching-Kun Chang, Arnold von Eckardstein, Lorenz Raber, Francois Mach, David Nanchen, Christian M. Matter, Luca Liberale, Giovanni G. Camici, Alexander Akhmedov, Chu-Huang Chen, Thomas F. Luscher
Summary: Changes in LDL electronegativity are associated with adverse outcomes in patients with ACS, including increased all-cause and cardiovascular mortality. The LDL lipidome is also affected by these alterations, and specific lipid species are independently associated with fatal events.
Article
Medicine, General & Internal
Sandeep Kumar, Wahid Ali, Sridhar Mishra, Akshyaya Pradhan, Rishi Sethi, Rashmi Kushwaha, Uma Shankar Singh, Marco Alfonso Perrone
Summary: The study revealed that sLOX-1 can serve as a biomarker for stable CAD and ACS, with potential in predicting the risk of future recurrence of coronary artery disease.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Simon Kraler, Florian A. Wenzl, Georgios Georgiopoulos, Slayman Obeid, Luca Liberale, Arnold von Eckardstein, Olivier Muller, Francois Mach, Lorenz Raeber, Sylvain Losdat, Martin O. Schmiady, Konstantinos Stellos, Kimon Stamatelopoulos, Giovanni G. Camici, Annie Srdic, Francesco Paneni, Alexander Akhmedov, Thomas F. Luescher
Summary: sLOX-1 is associated with fatal events and plaque progression in patients with acute coronary syndromes (ACS). Increased sLOX-1 levels predict mortality risk and coronary plaque progression beyond traditional risk factors.
EUROPEAN HEART JOURNAL
(2022)
Review
Medicine, General & Internal
Brian A. Bergmark, Njambi Mathenge, Piera A. Merlini, Marilyn B. Lawrence-Wright, Robert P. Giugliano
Summary: Although progress has been made in diagnosing and treating acute coronary syndromes, cardiovascular disease remains a leading cause of death globally. High-sensitivity troponin assays have allowed for rapid diagnosis in suspected cases. Dual antiplatelet therapy and additional preventive measures are recommended. The diagnosis and management of acute coronary syndromes continue to evolve, especially in response to the COVID-19 pandemic.
Article
Medicine, General & Internal
Asuka Ozaki, Katia Bravo-Jaimes, Carmen Smotherman, Dale F. Kraemer, Alfred Samura, Gladys P. Velarde
Summary: This study found that women and black patients with ACS were more likely to have higher HDL-C levels, and this was still the case in the absence of CAD or diabetes. Further research is needed to better understand the implications of HDL-C levels in different racial groups.
AMERICAN JOURNAL OF THE MEDICAL SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Tingting Li, Yingyi Zhang, Hongliang Cong
Summary: Evolocumab combination treatment for 8 weeks significantly improves plasma lipid profiles in ACS patients, and decreases the concentration of lipoprotein particles, potentially contributing to atherosclerosis pathogenesis.
BMC CARDIOVASCULAR DISORDERS
(2021)
Article
Medicine, Research & Experimental
Alexander Sorokin, Christin G. Hong, Angel M. Aponte, Elizabeth M. Florida, Jingrong Tang, Nidhi Patel, Irina N. Baranova, Haiou Li, Philip M. Parel, Vicky Chen, Sierra R. Wilson, Emily L. Ongstad, Anna Collen, Martin P. Playford, Thomas L. Eggerman, Marcus Y. Chen, Kazuhiko Kotani, Alexander Bocharov, Alan T. Remaley
Summary: In this study, we found that oxidized apolipoprotein B and oxidized ApoA-I are associated with high-risk coronary plaque features and progression in patients with cardiovascular disease.
Article
Cardiac & Cardiovascular Systems
Anselm K. Gitt, Klaus G. Parhofer, Ulrich Laufs, Winfried Maerz, W. Dieter Paar, Peter Bramlage, Nikolaus Marx
Summary: This study provides insights into the current treatment situation for patients in Germany who are at very high risk after experiencing an acute coronary syndrome (ACS) event. Despite receiving lipid-lowering therapy, many patients did not achieve recommended lipid targets by 12 months after the ACS event.
CLINICAL RESEARCH IN CARDIOLOGY
(2022)
Article
Medicine, General & Internal
Akshyaya Pradhan, Ravninder Kuka, Pravesh Vishwakarma, Wahid Ali, Marco Alfonso Perrone, Ferdinando Iellamo, Gaurav Chaudhary, Sharad Chandra, Rishi Sethi, Sudhanshu Dwivedi, Varun Narain, R. K. Saran
Summary: In ACS patients, age, sex, diabetes, smoking, and the severity of coronary artery disease did not significantly affect sd-LDL levels. However, prior statin usage was associated with lower sd-LDL levels, and diabetic patients had higher sd-LDL/lb-LDL ratios.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Medicine, General & Internal
Wenting Wang, Ronghua Luo, Dean Pei, Qi Huang, Xinyao Jin, Yuanhong Wu, Mingbin Xie, Shisheng Wang, Xiangqian Sui, Bin Shen
Summary: This study aims to evaluate the correlation between serum PCSK9 and platelet reactivity, as well as platelet-ox-LDL binding, in Chinese ACS patients. By measuring serum PCSK9, platelet activation, and platelet aggregation, and using flow cytometry to assess the binding of ox-LDL to platelets, this study will shed light on the relationship between PCSK9 and platelet reactivity, as well as the mechanism of PCSK9-mediated regulation of platelet reactivity.
Article
Medical Laboratory Technology
Hyun-Ki Kim, Jinyoung Hong, Sunyoung Ahn, Woochang Lee, Sail Chun, Won-Ki Min
Summary: The study found that in the general population, high levels of sdLDL-C are associated with high levels of oxLDL. However, the applicability of the equation for estimating sdLDL-C developed from the general population is limited in patients receiving DM treatment.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2023)
Article
Chemistry, Analytical
Soemwit Khongwichit, Piyawut Swangphon, Natthaphon Nanakorn, Aekkaraj Nualla-ong, Kiattawee Choowongkomon, Peter A. Lieberzeit, Suticha Chunta
Summary: In this study, a simple colorimetric assay was developed for sensitive and specific determination of oxidized low-density lipoprotein (oxLDL) using a selective aptamer coupled with salt-induced gold nanoparticle aggregation. The assay showed a linear response to oxLDL in the range of 0.002 to 0.5 μmol/L, with high selectivity and accurate recovery. When applied to artificial serum, the assay exhibited excellent correlation with conventional thiobarbituric acid reactive substances (TBARS), providing a rapid and convenient alternative for oxLDL detection.
Article
Medicine, General & Internal
Ting Hung, Jin-You Jhan, Jian-Hong Lin, Kun-Ta Yang, Bang-Gee Hsu, Jui-Chih Chang
Summary: This study aimed to evaluate the relationship between serum MDA-oxLDL levels and CAVI in patients with triple-vessel coronary artery disease who underwent CABG surgery. The results showed a positive correlation between MDA-oxLDL levels and CAVI levels.
JOURNAL OF CLINICAL MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Cristina Barale, Elena Melchionda, Alessandro Morotti, Isabella Russo
Summary: PCSK9 plays a key role in lipoprotein metabolism and atherosclerosis, with potential cardiovascular benefits from its inhibition. Currently, anti-PCSK9 antibodies and small interfering RNA are effective therapies to reduce LDL-C levels and attenuate cardiovascular disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Rheumatology
Maria G. Tektonidou, Evrydiki Kravvariti, Nikolaos Vlachogiannis, Georgios Georgiopoulos, Aimilia Mantzou, Petros P. Sfikakis, Konstantinos Stellos, Kimon Stamatelopoulos
Summary: In patients with Antiphospholipid Syndrome (APS), elevated plasma levels of Aβ40 are associated with adverse thrombo-inflammatory profile, and independently correlated with recurrent arterial events. Aβ40 levels provide additional value in improving the discrimination ability for recurrent arterial thromboses over the validated risk score aGAPSS in APS.
Editorial Material
Cardiac & Cardiovascular Systems
Monika Gladka, Konstantinos Stellos
CARDIOVASCULAR RESEARCH
(2021)
Article
Hematology
Irene Lambrinoudaki, Dimitrios Delialis, Georgios Georgiopoulos, Simon Tual-Chalot, Nikolaos I. Vlachogiannis, Raphael Patras, Evmorfia Aivalioti, Eleni Armeni, Areti Augoulea, Nikolaos Tsoltos, Anastasia Soureti, Konstantinos Stellos, Kimon Stamatelopoulos
Summary: Elevated levels of Alpha beta 1-40 may be associated with the progression of atherosclerosis in postmenopausal women. Patterns of persistently high or increasing Alpha beta 1-40 levels could be linked to accelerated subclinical atherosclerosis progression.
THROMBOSIS AND HAEMOSTASIS
(2021)
Review
Pharmacology & Pharmacy
Elias Rawish, Manuela Sauter, Reinhard Sauter, Henry Nording, Harald F. Langer
Summary: This review explores the mutual relationship between immune activation and thrombus formation mechanisms, particularly focusing on how the complement system can modulate platelet activation. While some components of the complement system have been associated with platelets and shown to be functionally active in the micromilieu of platelet activation, the exact regulatory mechanisms and the consequences on tissue inflammation, damage, or recovery are still to be fully understood.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Multidisciplinary Sciences
Henry Nording, Lasse Baron, David Haberthuer, Frederic Emschermann, Matthias Mezger, Manuela Sauter, Reinhard Sauter, Johannes Patzelt, Kai Knoepp, Anne Nording, Moritz Meusel, Roza Meyer-Saraei, Ruslan Hlushchuk, Daniel Sedding, Oliver Borst, Ingo Eitel, Christian M. Karsten, Robert Feil, Bernd Pichler, Jeanette Erdmann, Admar Verschoor, Emmanouil Chavakis, Triantafyllos Chavakis, Philipp von Hundelshausen, Joerg Koehl, Meinrad Gawaz, Harald F. Langer
Summary: This study reveals that platelets negatively modulate revascularization through C5aR1 activation, inhibiting endothelial cell functions. By identifying the C5a/C5aR1-CXCL4 axis in platelets, a mechanism for controlling tissue neovascularization has been uncovered.
NATURE COMMUNICATIONS
(2021)
Article
Hematology
Malgorzata Cebo, Kristina Dittrich, Xiaoqing Fu, Mailin C. Manke, Frederic Emschermann, Johannes Rheinlaender, Hendrik von Eysmondt, Nerea Ferreiros, Jessica Sudman, Alexander Witte, Lisann Pelzl, Oliver Borst, Tobias Geisler, Dominik Rath, Tamam Bakchoul, Meinrad Gawaz, Tilman E. Schaeffer, Michael Laemmerhofer, Madhumita Chatterjee
Summary: Platelet ACKR3/CXCR7 surface expression is enhanced in coronary artery disease (CAD) patients, and it influences the prognosis. This study shows that ACKR3/CXCR7 regulates thromboinflammatory response by impacting the platelet lipidome. The findings suggest that ACKR3/CXCR7 may be a potential therapeutic target for cardiovascular diseases and CAD.
Article
Biochemistry & Molecular Biology
Jeremy A. Nestele, Anne-Katrin Rohlfing, Valerie Dicenta, Alexander Bild, Daniela Eissler, Frederic Emschermann, Marcel Kremser, Konstantin Krutzke, Tilman E. Schaeffer, Oliver Borst, Moran Levi, Netanel Korin, Meinrad Paul Gawaz
Summary: Nanoparticles provide a novel approach for targeted antithrombotic therapy, delivering drugs to specific sites with low side effects. In this study, nanoparticles combined with established proteins successfully inhibited platelet aggregation and thrombus formation, indicating a promising therapeutic strategy for antithrombotic treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Manuela Sauter, Reinhard J. Sauter, Henry Nording, Chaolan Lin, Marcus Olbrich, Stella Autenrieth, Christian Gleissner, Martin Thunemann, Nadia Otero, Esther Lutgens, Zouhair Aherrahrou, Dennis Wolf, Lars Zender, Sven Meuth, Robert Feil, Harald F. Langer
Summary: This study investigated the role of CD11c(+) cells in atherosclerosis. The results showed that CD11c(+) cells alleviate atherosclerosis by secreting ApoE, and the level of ApoE derived from CD11c(+) cells is associated with the burden of atherosclerotic plaques.
Article
Immunology
Henry Nording, Manuela Sauter, Chaolan Lin, Rebecca Steubing, Sven Geisler, Ying Sun, Joel Niethammer, Frederic Emschermann, Yunmei Wang, Barbara Zieger, Bernhard Nieswandt, Christoph Kleinschnitz, Daniel I. Simon, Harald F. Langer
Summary: Recent evidence supports the interaction between platelets and dendritic cells (DCs), but the molecular mechanisms underlying this heterotypic cell cross-talk remain largely unknown. In this study, the researchers found that activated platelets can enhance the binding between human monocyte-derived DCs and platelets in a dynamic incubation model. Inhibition of CD11b and GPIba significantly reduced the adhesion between DCs and platelets in vitro, and the same inhibition also decreased platelet-mediated DC adhesion to the injured arterial wall in vivo.
JOURNAL OF IMMUNOLOGY
(2022)
Review
Physiology
M. Sauter, H. F. Langer
Summary: This article investigates the contribution of innate immune mechanisms to atherosclerosis and their interaction with molecules of cholesterol homeostasis. Recent research has identified potential roles of previously overlooked cell types, such as platelets and dendritic cells, in this chronic disease. However, specific therapeutic approaches targeting these immune mechanisms have not yet been successfully introduced into everyday clinical practice.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Hematology
Henry Nording, Lasse Baron, Manuela Sauter, Antje Luebken, Elias Rawish, Rebecca Szepanowski, Jacob von Esebeck, Ying Sun, Hossein Emami, Moritz Meusel, Roza Saraei, Nancy Schanze, Sivahari Prasad Gorantla, Nikolas von Bubnoff, Tobias Geisler, Philipp von Hundelshausen, Konstantinos Stellos, Jens Marquardt, Christian D. Sadik, Joerg Koehl, Daniel Duerschmied, Christoph Kleinschnitz, Harald F. Langer
Summary: Platelets modulate angiogenesis in ischemic tissue through activation of C5a receptor 1 (C5aR1). The presence of C5aR1-expressing platelets is increased in ischemic hindlimb, and ischemia-driven angiogenesis is significantly improved in C5aR1-/- mice. The inhibition of angiogenesis by platelets is mediated by the release of antiangiogenic CXCL4 through a paracrine mechanism, which depends on the activation of C5a-C5aR1 axis.
Article
Immunology
Rebecca D. Steubing, Fabian Szepanowski, Christina David, Ayan Mohamud Yusuf, Stine Mencl, Anne-Kathrin Mausberg, Harald F. Langer, Manuela Sauter, Cornelius Deuschl, Michael Forsting, Anke C. Fender, Dirk M. Hermann, Ana I. Casas, Friederike Langhauser, Christoph Kleinschnitz
Summary: Platelets play a critical role in thrombus formation and inflammation during acute ischemic stroke, but their contribution to long-term tissue reorganization and functional recovery appears to be marginal.
BRAIN, BEHAVIOR, & IMMUNITY - HEALTH
(2022)
Article
Biochemical Research Methods
Manuela Sauter, Reinhard J. Sauter, Henry Nording, Marcus Olbrich, Frederic Emschermann, Harald F. Langer
Summary: This article describes an in vitro approach to analyze the role of CD11c+ cells and CD11c+-derived ApoE in atherosclerosis. The method involves harvesting mouse bone marrow, culturing the cells, treating them with differentiation factors, and collecting the cells after removing undesirable populations. This protocol can be adapted for different diseases and used to analyze cell-specific molecules.
Article
Biochemical Research Methods
Manuela Sauter, Reinhard J. Sauter, Marcus Olbrich, Martin Thunemann, Susanne Feil, Robert Feil, Harald F. Langer
Summary: In this article, an in vivo approach to visualize CD11c+ cells in atherosclerosis is described. The authors use X-Gal staining to analyze plaque composition and cell-specific molecules as an alternative method. By breeding mice with LacZ knockin and CD11ccre recombinase onto an ApoE-/- background, the interested cell type in the plaques can be visualized by X-Gal staining. This approach allows for the examination of various immune cells in atherogenesis.