Article
Immunology
Serena Li-Sue Yan, Il-Young Hwang, Olena Kamenyeva, Juraj Kabat, Ji Sung Kim, Chung Park, John H. Kehrl
Summary: This study demonstrates the critical role of RGS protein/G alpha(i) interactions in shaping neutrophil responses to chemoattractant receptor activation. Mutant neutrophils with disabled RGS protein-G alpha(i2) interactions show impaired trafficking and inflammatory responses, providing insights into the importance of these interactions in normal neutrophil function.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Multidisciplinary Sciences
Lai Shi, Karen Aymonnier, Denisa D. Wagner
Summary: PAD4 catalyzes posttranslational modification by converting protein arginine to citrulline, promoting histone H4-induced NET formation, while citrullinated histone H4 may reduce NET formation, potentially playing a role in the pathology of NETs.
Article
Biochemistry & Molecular Biology
Gulmira oezek, Igor A. Schepetkin, Moldir Yermagambetova, Temel oezek, Liliya N. Kirpotina, Shyryn S. Almerekova, Saule I. Abugalieva, Andrei I. Khlebnikov, Mark T. Quinn
Summary: This study analyzed the chemical composition and innate immunomodulatory activity of essential oils isolated from eight Juniperus species found in Montana and Kazakhstan. The study identified cedrol as a novel neutrophil agonist that can desensitize cells to subsequent stimulation by N-formyl peptide, suggesting potential kinase targets for cedrol. Several essential oil samples contained high levels of cedrol, inducing intracellular Ca2+ mobilization in human neutrophils which inhibited chemotaxis to N-formyl peptide.
Article
Plant Sciences
Igor A. Schepetkin, Gulmira Ozek, Temel Ozek, Liliya N. Kirpotina, Polina Kokorina, Andrei Khlebnikov, Mark T. Quinn
Summary: This study found that essential oils from black poplar and propolis, as well as nerolidol, can modulate the immune response and chemotaxis of human neutrophils.
Article
Biochemistry & Molecular Biology
Francisco Sadras, Teneale A. Stewart, Melanie Robitaille, Amelia A. Peters, Priyakshi Kalita-de Croft, Patsy S. Soon, Jodi M. Saunus, Sunil R. Lakhani, Sarah J. Roberts-Thomson, Gregory R. Monteith
Summary: The role of calcium signalling pathways in inducing CAF phenotype has not been fully explored. A CAF model was generated by stimulating normal human mammary fibroblast cells with TGF beta 1, leading to altered calcium influx pathways. Upregulation of VGCCs Ca(V)1.2 and Ca(V)3.2 was observed in both the model and patient-derived breast CAFs, and inhibiting these channels impaired CAF activation. Targeting calcium signalling in breast CAFs could have a therapeutic potential.
Article
Biology
Andrew T. Landau, Pojeong Park, J. David Wong-Campos, He Tian, Adam E. Cohen, Bernardo L. Sabatini
Summary: This study reveals that back-propagating action potentials (bAPs) fail to evoke calcium influx in specific dendritic branches of mouse cortical layer 2/3 pyramidal cells, despite evoking it in sister branches. The branch-specific compartmentalization of bAP-dependent calcium signals may provide a mechanism for neurons to diversify synaptic tuning across the dendritic tree.
Article
Biochemistry & Molecular Biology
Igor A. Schepetkin, Gulmira Ozek, Temel Ozek, Liliya N. Kirpotina, Andrei Khlebnikov, Mark T. Quinn
Summary: This study investigated the chemical composition and immunomodulatory activity of essential oils from Grindelia squarrosa. The main components of the oils were alpha-pinene, limonene, and borneol. The oils were found to activate human neutrophils and inhibit their responses to agonists.
Article
Biochemistry & Molecular Biology
Dandan Huang, Qihan Ding, Shenbao Chen, Shouqin Lu, Yan Zhang, Mian Long
Summary: E-selectins negatively regulate neutrophil transmigration by altering endothelial plasticity, downregulating cortactin and Arp2/3 activation, and enhancing VE-cadherin adhesive function. The findings also highlight the overlapping functions of redundant P-selectins in neutrophil adhesion and transmigration, contributing to better understanding of actin remodeling and junctional integrity in endothelial cells mediating leukocyte TEM.
Article
Cell Biology
Bowen Liu, Yuan Jin, Jingyi Yang, Yue Han, Hui Shan, Mantang Qiu, Xuyang Zhao, Anhang Liu, Yan Jin, Yuxin Yin
Summary: Lung extracellular vesicles (EVs) promote chemotaxis of neutrophils in the bone marrow by delivering double stranded DNA (dsDNA). Type II alveolar epithelial cells are identified as the main source of these lung EVs. Furthermore, lung EVs accumulate in the bone marrow and enhance neutrophil recruitment under inflammation conditions. Additionally, lung EV-DNA stimulates neutrophils to release the chemokines CXCL1 and CXCL2 through DNA-TLR9 signaling.
JOURNAL OF EXTRACELLULAR VESICLES
(2022)
Article
Plant Sciences
Qian Zhang, Tao Song, Can Guan, Yingjie Gao, Jianchao Ma, Xiangyang Gu, Zhiguang Qi, Xiaoji Wang, Zhengge Zhu
Summary: This study revealed the crucial role of rice annexin OsANN4 in the ABA response, partially by modulating ROS production, mediating Ca2+ influx, or interacting with OsCDPK24.
Article
Biochemistry & Molecular Biology
Shuxiang Yu, Xiaoke Geng, Huibing Liu, Yunyun Zhang, Xiumei Cao, Baojie Li, Jianshe Yan
Summary: Peritoneal inflammation is a major cause of treatment failure in patients with kidney failure who undergo peritoneal dialysis. The molecular mechanisms of neutrophil recruitment in peritonitis are not fully understood. The deletion of the Elmo1 gene causes defects in the chemotaxis and adhesion of neutrophils, and targeting ELMO1 may be a potential therapeutic strategy for peritonitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
David Alexander Christian Messerer, Hanna Schmidt, Manfred Frick, Markus Huber-Lang
Summary: Neutrophil granulocytes play a crucial role in innate immunity but can lead to cellular dysfunction and multiple organ failure when excessively stimulated. Following stimulation, they respond with defined physiological patterns involving ion and water fluxes. Ion transport proteins are critical for the immune functions of neutrophils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Sanja Skulj, Zlatko Brkljaca, Jurgen Kreiter, Elena E. Pohl, Mario Vazdar
Summary: Molecular dynamics simulations of uncoupling proteins present a challenge due to the lack of available structural data. In this study, a combination of homology modelling and MD simulations of UCP2 revealed a structure impermeable to water. Additionally, results from conductance measurements in model membranes suggest that the UCP2 structure modeled from ANT protein possesses key functional elements related to proton transport.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Igor A. Schepetkin, Gulmira Ozek, Temel Ozek, Liliya N. Kirpotina, Andrei Khlebnikov, Mark T. Quinn
Summary: Rhododendron extracts contain various compounds and essential oils from flowers and leaves have different chemical compositions, affecting intracellular Ca2+ mobilization in human neutrophils and microglial cells. The essential oils may have immunotherapeutic properties and modulate innate immune responses, possibly through inhibition of neutrophil migration.
Article
Chemistry, Medicinal
Igor A. Schepetkin, Gulmira Ozek, Temel Ozek, Liliya N. Kirpotina, Andrei Khlebnikov, Robyn A. Klein, Mark T. Quinn
Summary: This study isolated essential oils from five Artemisia species and found that these oils have immunosuppressive activity on human neutrophils, with the essential oils from A. dracunculus being the most effective. Further analysis identified farnesene as a potential anti-inflammatory modulator of human neutrophils.