Article
Chemistry, Physical
Anna Mularski, Stine Lauritzen Sonder, Anne Sofie Busk Heitmann, Jesper Nylandsted, Adam Cohen Simonsen
Summary: The study reveals that ANXA4 and ANXA5 colocalize at the damage site of MCF7 cells during repair, with their (1:1) mixture inducing membrane rolling with an intermediate time constant. Unlike the crystal lattices formed by pure annexins, the (1:1) mixture generates a random arrangement of trimers, showing that curvature induction is the functional property of annexin mixtures in PMR rather than crystal formation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Oncology
Alexis Woodward, Gabriela N. F. Faria, Roger G. Harrison
Summary: Identifying a universal biomarker for cancer treatment remains a major challenge. Phosphatidylserine is highly expressed in cancer cells and the vasculature, and the native binding partner ANXA5 can deliver chemotherapies to the tumor microenvironment.
Review
Biochemistry & Molecular Biology
Luiz Philipe de Souza Ferreira, Rafael Andre da Silva, Cristiane D. Gil, Michael J. Geisow
Summary: The human genome codes for 12 annexins with similar membrane-binding cores and unique amino termini. Annexins are present in almost all eukaryotes and their ability to bind to membrane lipid bilayers is key to their retention in eukaryotic molecular cell biology. Annexin genes have diverse functions and are important in responding to cell and tissue stress. In humans, specific annexins, such as AnxA1, 2, 5, and 6, are being extensively studied for their roles in various pathologies, including cancer.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2023)
Article
Engineering, Biomedical
Sung Eun Lee, Chan Mi Lee, Ji Eun Won, Gun-Young Jang, Ju Hyeong Lee, Sang Hyeon Park, Tae Heung Kang, Hee Dong Han, Yeong-Min Park
Summary: This study demonstrates a selective nanocarrier system that enhances cytotoxic T cell immunity against tumor-specific antigens, increases survival rates of immune-activating cells, and reduces tumor mass by decreasing immune-suppressive cells and anti-inflammatory cytokines. Combining the nanocarrier system with other ICIs further amplifies the anticancer immunity of interferon gamma+ (IFN-gamma) CD8+ T cells.
Article
Chemistry, Medicinal
Brent J. Tschirhart, Xiangru Lu, Janice Gomes, Arundhasa Chandrabalan, Gillian Bell, David A. Hess, Guangxin Xing, Hong Ling, Dylan Burger, Qingping Feng
Summary: Sepsis is a dysregulated immune response to infection and a leading cause of mortality. Recombinant human Annexin A5 (Anx5) treatment has been shown to inhibit pro-inflammatory cytokine production and improve survival in sepsis models. It blocks the inflammatory response induced by activated platelets and microvesicles in endothelial cells through phosphatidylserine binding.
Article
Obstetrics & Gynecology
Xiaojie Wang, Yarui Dai, Jialu Zhang, Xin Li
Summary: This study found that ANXA5 can inhibit proliferation and metastasis of cervical cancer cells by regulating the PI3K/Akt signaling pathway.
ARCHIVES OF GYNECOLOGY AND OBSTETRICS
(2023)
Article
Biochemistry & Molecular Biology
Emily Sze-Wan Wong, Renkai Li, Jingjing Li, Chengwen Zheng, Polly Ho-Ting Shiu, Panthakarn Rangsinth, Sai-Wang Seto, George Pak-Heng Leung
Summary: This study investigated the involvement of equilibrative nucleoside transporter 4 (ENT4) in adenosine transport in cardiomyocytes under simulated ischemic conditions. The results showed that inhibition of ENT4 could protect cardiomyocytes against ischemia-reperfusion injury. These findings suggest that ENT4 may play a role in adenosine transport in cardiomyocytes under ischemic conditions.
MOLECULAR BIOLOGY REPORTS
(2022)
Article
Immunology
Yalong Gao, Hejun Zhang, Jiwei Wang, Fanjian Li, Xiaotian Li, Tuo Li, Cong Wang, Lei Li, Ruilong Peng, Li Liu, Weiyun Cui, Shu Zhang, Jianning Zhang
Summary: In this study, we investigated whether inhibiting ferroptosis and neuroinflammation using A5 could ameliorate traumatic brain injury. The results showed that A5 improved neurological deficits, brain edema, blood-brain barrier disruption, and reduced neuronal apoptosis and ferroptosis. It also regulated the NF-kB/HMGB1 pathway and the Nrf2/HO-1 antioxidant system to decrease neuroinflammation and oxidative stress damage.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Genetics & Heredity
Nina Rogenhofer, Arseni Markoff, Xenia Ennerst, Nadja Bogdanova, Christian Thaler
Summary: Carriage of the M2/ANXA5 haplotype is associated with a significantly increased risk of recurrent implantation failure (RIF) for both maternal and paternal carriers, providing new insights into the pathogenesis of RIF and identifying relevant risk groups.
JOURNAL OF ASSISTED REPRODUCTION AND GENETICS
(2021)
Article
Cell Biology
Celine Gounou, Flora Bouvet, Benjamin Liet, Valerie Prouzet-Mauleon, Lena d'Agata, Etienne Harte, Francoise Argoul, Geraldine Siegfried, Richard Iggo, Abdel-Majid Khatib, Anthony Bouter
Summary: ANXA5 and ANXA6 are crucial components of the membrane repair machinery in MDA-MB-231 cells and are correlated with the invasiveness of breast cancer cells. High expression of ANXA5 and ANXA6 predicts poor prognosis in high-grade lung, gastric, and breast cancers. Genetic inhibition of ANXA5 and ANXA6 in zebrafish significantly reduces tumor cell dissemination.
BIOLOGY OF THE CELL
(2023)
Article
Endocrinology & Metabolism
Takuya Murata, Shuichi Chiba, Mitsumori Kawaminami
Summary: GnRH stimulates the expression of ANXA1 and ANXA5 mRNA, with ANXA1 showing a much greater response. These annexins play an important role in GnRH action at gonadotropes.
Article
Endocrinology & Metabolism
Takuya Murata, Shuichi Chiba, Mitsumori Kawaminami
Summary: The study showed that GnRH analog GnRHa can stimulate the mRNA expression levels of ANXA1 and A5 via PKC activation, with ANXA1 showing a much greater increase compared to ANXA5. TPA also dose-dependently increased the expression of ANXA1 and A5, with ANXA1 showing a higher response than ANXA5. The results suggest a close relationship between these annexins and a significant role for ANXA1 in GnRH action at gonadotropes.
Review
Nutrition & Dietetics
Torsten Bohn, Julian Hellman-Regen, Angel R. de Lera, Volker Boehm, Ralph Ruehl
Summary: In the last century, the concept of vitamin A2 pathway and the existence of vitamin A1/provitamin A1 were identified along with the well-known vitamin A. This review introduces the concept of vitamin A5/X, which is considered a direct nutritional precursor of retinoic acid receptors and serves as a parallel concept to vitamin A1/ provitamin A1. The aim is to summarize the current knowledge and gaps regarding vitamin A5/X and propose adapted nutritional regulations.
NUTRITION & METABOLISM
(2023)
Article
Pharmacology & Pharmacy
Jianmin Chen, Silvia Oggero, Chiara Cecconello, Jesmond Dalli, Hedayatullah Hayat, Ahmad Hjiej Andaloussi, Samra Sanni, Thomas E. N. Jonassen, Mauro Perretti
Summary: RTP-026 has cardio-protective and immuno-regulatory properties, reducing infarct size in rat acute myocardial infarction by modulating immune cell responses and decreasing cardiac damage.
PHARMACOLOGICAL RESEARCH
(2023)
Article
Immunology
Senjuti Dasgupta, Priyanka Maity, Uma Banerjee, Partha Mukhopadhyay, Saswata Saha, Biplab Das
Summary: The study revealed significantly reduced levels of ANXA5 and apelin in the placentae of patients with pre-eclampsia compared to controls. Furthermore, the expression levels of these markers were significantly associated with neonatal resuscitation and the requirement of treatment in the neonatal care unit for sick infants.
INDIAN JOURNAL OF MEDICAL RESEARCH
(2021)
Article
Rheumatology
Marc H. A. Jansen, Christien Rondaan, Geertje E. Legger, Kirsten Minden, Yosef Uziel, Natasa Toplak, Despoina Maritsi, Lotte van den Berg, Guy A. M. Berbers, Patricia Bruijning, Yona Egert, Christophe Normand, Marc Bijl, Helen E. Foster, Isabelle Kone-Paut, Carine Wouters, Angelo Ravelli, Ori Elkayam, Nicolaas M. Wulffraat, Marloes W. Heijstek
Summary: This article updates the EULAR recommendations on vaccination for pedAIIRD patients, providing guidelines on vaccination principles, special circumstances, and the safety for immunosuppressed patients.
ANNALS OF THE RHEUMATIC DISEASES
(2023)
Article
Oncology
Maarten J. van der Doelen, Irma M. Oving, Dirk N. J. Wyndaele, Jean-Paul van Basten, Frederiek Terheggen, Addy C. M. van de Luijtgaarden, Wim J. G. Oyen, W. Dick van Schelven, Franchette van den Berkmortel, Niven Mehra, Marcel J. R. Janssen, Judith B. Prins, Winald R. Gerritsen, Jose A. E. Custers, Inge M. van Oort
Summary: This study evaluated the health-related quality of life, psychological distress, and fatigue in patients with metastatic castration-resistant prostate cancer (mCRPC) treated with radium-223. The study found that patients who discontinued radium-223 therapy had worse HR-QoL, psychological distress, and fatigue at baseline and more frequent deterioration over time compared to patients who completed therapy.
PROSTATE CANCER AND PROSTATIC DISEASES
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Elizabeth J. de Koster, Adriana C. H. van Engen-van Grunsven, Johan Bussink, Cathelijne Frielink, Lioe-Fee de Geus-Oei, Benno Kusters, Hans Peters, Wim J. G. Oyen, Dennis Vriens
Summary: This study explored the association between [F-18]FDG uptake and the quantitative expression of immunohistochemical markers related to glucose metabolism, hypoxia, and cell proliferation in benign and malignant thyroid nodules of indeterminate cytology. The results showed that the expression of GLUT1, HK2, and MCT4 increased in [F-18]FDG-positive benign nodules and thyroid carcinomas as compared to [F-18]FDG-negative benign nodules, and VEGF expression was higher in thyroid carcinomas.
MOLECULAR IMAGING AND BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Kourosh Ahmadzadeh, Marie Pereira, Margot Vanoppen, Eline Bernaerts, Jeong-Hun Ko, Tania Mitera, Christy Maksoudian, Bella B. Manshian, Stefaan Soenen, Carlos D. Rose, Patrick Matthys, Carine Wouters, Jacques Behmoaras
Summary: Macrophages can undergo cell fusion and multinucleation to form multinucleated giant cells. Comparative transcriptomics and functional assays reveal that multinucleation causes downregulation of macrophage identity and specialization in functionality, such as enhanced phagocytosis and mitochondrial function.
Letter
Radiology, Nuclear Medicine & Medical Imaging
Dennis Vriens, Elizabeth J. de Koster, Lioe-Fee de Geus-Oei, Wim J. G. Oyen, EfFECTS trial consortium
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Article
Pediatrics
Lien De Somer, Brigitte Bader-Meunier, Sylvain Breton, Sara Brachi, Carine Wouters, Francesco Zulian
Summary: This study retrospectively collected data on the clinical features, radiological manifestations, and disease course of dry synovitis (DS) patients and compared them with rheumatoid factor negative polyarticular juvenile idiopathic arthritis (RFneg-polyJIA) patients. The results showed that DS patients had a higher number of joint involvement, more severe joint contractures, and a longer diagnostic delay compared to RFneg-polyJIA patients.
PEDIATRIC RHEUMATOLOGY
(2023)
Article
Immunology
Karen Yu, Luna Dillemans, Mieke Gouwy, Helena Bessa, Mieke Metzemaekers, Erik Martens, Patrick Matthys, Xavier Bossuyt, Patrick Verschueren, Carine Wouters, Lien De Somer, Paul Proost
Summary: In this study, a fluorescent in vitro assay was improved to characterize endogenous PAD activity in complex samples. The results showed similar levels of PAD activity in synovial fluids of rheumatoid arthritis (RA) and juvenile idiopathic arthritis (JIA) patients, while citrullination was limited in joints of patients with gout or Lyme's disease. Interestingly, a higher level of extracellular citrullination was only found in blood of anti-CCP-positive RA patients.
FRONTIERS IN IMMUNOLOGY
(2023)
Correction
Biochemistry & Molecular Biology
Kourosh Ahmadzadeh, Marie Pereira, Margot Vanoppen, Eline Bernaerts, Jeong-Hun Ko, Tania Mitera, Christy Maksoudian, Bella B. Manshian, Stefaan Soenen, Carlos D. Rose, Patrick Matthys, Carine Wouters, Jacques Behmoaras
Article
Radiology, Nuclear Medicine & Medical Imaging
H. Adams, E. M. W. van de Garde, D. J. Vugts, J. C. Grutters, Wim. J. G. Oyen, R. G. Keijsers
Summary: This study investigates the use of imaging CD20-positive cells in predicting the response to anti-CD20 treatment for immune-mediated interstitial lung disease. PET/CT imaging was performed on 21 patients, and it was found that the uptake of [89Zr]-rituximab in the lungs correlated with changes in pulmonary function test results and treatment outcomes. Patients with a higher uptake of [89Zr]-rituximab had better response to treatment. Therefore, [89Zr]-rituximab PET imaging may serve as a potential predictive biomarker for anti-CD20 therapy.
EUROPEAN JOURNAL OF NUCLEAR MEDICINE AND MOLECULAR IMAGING
(2023)
Letter
Medicine, General & Internal
Ellen De Langhe, Sien Van Loo, Bert Malengier-Devlies, Mieke Metzemaekers, Frederik Staels, Jessica Vandenhaute, Nathalie Berghen, Raf Sciot, Anniek Corveleyn, Olga Tsuiko, Mieke Gouwy, Jan Lenaerts, Patrick Verschueren, Carine H. Wouters, Paul Proost, Patrick Matthys, Eric Legius, Rik Schrijvers
Summary: The article reports a case of adult-onset inflammatory disease. It is revealed that two somatic genetic events are responsible for the disease and its onset in adulthood.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Article
Rheumatology
Hermine Brunner, Nikolay Tzaribachev, Ingrid Louw, Inmaculada Calvo Penades, Francisco Avila-Zapata, Gerd Horneff, Ivan J. Foeldvari, Daniel Kingsbury, Maria Eliana Paz Gastanaga, Carine Wouters, Johannes Breedt, Robert Wong, Margarita Askelson, Joe Zhuo, Alberto J. Martini, Daniel Lovell, Nicolino Ruperto
Summary: This study aimed to investigate the frequency and trajectories of individual patients with polyarticular-course juvenile idiopathic arthritis (JIA) achieving various composite end points on abatacept treatment. The results showed that patients achieved composite end points of low disease activity (LDA) with minimal pain, LDA with Childhood Health Assessment Questionnaire disability index score of 0, and 50% improvement in American College of Rheumatology criteria for JIA (ACR50) with minimal pain at 4 months. The proportion of patients meeting these end points increased over 21 months of abatacept treatment.
ARTHRITIS CARE & RESEARCH
(2023)
Article
Oncology
Elizabeth J. de Koster, Willem E. Corver, Lioe-Fee de Geus-Oei, Wim J. G. Oyen, Dina Ruano, Abbey Schepers, Marieke Snel, Tom van Wezel, Dennis Vriens, Hans Morreau
Summary: This study described the patterns of copy number alterations (CNA) in benign and malignant oncocytic cell thyroid neoplasms (OCN) using a next-generation sequencing (NGS) panel. The study found that the CNA patterns differed between benign and malignant cases, and suggested the application of CNA-LOH analysis using the NGS panel in the molecular diagnostics of OCN.
ENDOCRINE-RELATED CANCER
(2023)
Article
Pediatrics
M. Wallimann, K. Bouayed, E. Cannizzaro, D. Kaiser, A. Belot, E. Merlin, S. Poignant, C. Wouters, F. Hofer, T. Saurenmann, A. Koryllou, R. Carlomagno, M. Mejbri, M. Hofer, K. Theodoropoulou
Summary: This study aims to identify predictors of disease evolution in patients with systemic juvenile idiopathic arthritis (sJIA). The results suggest that early disease onset, persistence of arthritis in the first year, and the use of synthetic DMARD may predict a persistent disease course.
PEDIATRIC RHEUMATOLOGY
(2023)
Meeting Abstract
Cardiac & Cardiovascular Systems
Matthias M. Engelen, Quentin Van Thillo, Albrecht Betrains, Iwein Gyselinck, Caroline P. Martens, Val Erie Spalart, Anna Ockerman, Caroline Devooght, Joost Wauters, Jan Gunst, Carine Wouters, Christophe Vandenbriele, Steffen Rex, Laurens Liesenborghs, Alexander Wilmer, Philippe Meersseman, Greet Van den Berghe, Dieter Dauwe, Ann Belmans, Michiel Thomeer, Tom Fivez, Dieter Mesotten, David Ruttens, Luc Heytens, Ilse Dapper, Sebastiaan Tuyls, Brecht De Tavernier, Peter Verhamme, Thomas Vanassche
Article
Immunology
Luna Dillemans, Youri Bekhuis, Albrecht Betrains, Karen Yu, Maarten van Hemelen, Noemie Portner, Lien De Somer, Patrick Matthys, Jeroen Breckpot, Thomas Tousseyn, Marijke Peetermans, Paul Proost, Carine Wouters, Steven Vanderschueren
Summary: We report a fatal case of a 20-year-old woman with refractory adult-onset Still's disease accompanied by fulminant macrophage activation syndrome and atypical hemolytic uremic syndrome. This case highlights the importance of prompt genetic assessment of complement-encoding alleles, in addition to HLH-related genes, in patients with severe adult-onset Still's disease with recurrent macrophage activation syndrome and features of thrombotic microangiopathy.
CLINICAL IMMUNOLOGY
(2023)