A novel posavirus-related single-stranded RNA virus from fish (Cyprinus carpio)

The complete genome sequence of a novel +ssRNA virus, provisionally named fisavirus 1 (strain HAL1/fisa-ssRNAV, KM434233), identified from a freshwater carp (Cyprinus carpio), was determined using viral metagenomics and RT-PCR. The 8712-nt-long genome of HAL1/fisa-ssRNAV shares the same genome organization (i.e., a potential single ORF and N-terminal Hel-Pro-Pol replication domains followed by the structural proteins) and distant phylogenetic relationship to the currently unclassified posaviruses, which suggests that these viruses could belong to different genera in a novel family of the order Picornavirales.