Article
Infectious Diseases
Zida Zhen, Linlin Zhang, Qi Li, Yun Zhu, Xiaohuan Wang, Xiaoyan Fu, Junhong Ai, Ran Wang, Zhengde Xie, Shuxuan Ma
Summary: Monkeypox virus (MPXV), a type of Orthopoxvirus, caused a global outbreak in 2022, raising widespread concerns. Cross-reactive antibodies induced by vaccinia inoculation can protect against reinfection by MPXV. The long-term seroprevalence of VTT-vaccinated populations in China, where the VTT strain was widely administered before the 1980s, remains uncertain. Our study found that 31.8% (75/236) of individuals vaccinated with VTT four decades ago still had cross-reactive IgG antibodies against MPXV, suggesting potential long-term protection.
INFECTION GENETICS AND EVOLUTION
(2023)
Review
Multidisciplinary Sciences
Rafael Borges Rosa, Emilene Ferreira de Castro, Murilo Vieira da Silva, Denise Caroline Paiva Ferreira, Ana Carolina Gomes Jardim, Igor Andrade Santos, Mikaela dos Santos Marinho, Flavia Batista Ferreira Franca, Lindomar Jose Pena
Summary: The emergence and rapid spread of monkeypox virus (MPXV) outside of endemic areas during the COVID-19 pandemic has created a global health emergency. Scientists have been working on developing in vitro and in vivo models for MPXV research. In vitro models, including 2D and 3D cultures, are cost-effective and can be controlled, but may not fully represent physiological conditions. Therefore, in vivo models are being characterized to meet the demand for studying MPXV. This review summarizes the existing MPXV models and discusses their contributions to understanding pathogenesis, replication, transmission, and antiviral treatment.
Article
Chemistry, Medicinal
Yanrong Zhou, Qianpeng Wang, Qi Ying, Xiaomei Zhang, Ting Ye, Kan Chen, Gongchu Li
Summary: This study compares the efficacy of oncolytic vaccinia viruses carrying different marine lectins in breast cancer cells and finds that oncoVV-AVL shows the highest anti-tumor effect. Further studies show that apoptosis and replication can enhance the cytotoxicity of oncoVV-lectins, but the mechanisms differ in different cell types.
Article
Multidisciplinary Sciences
Brittany Jasperse, Caitlin M. O'Connell, Yuxiang Wang, Paulo H. Verardi
Summary: Researchers developed replication-inducible vaccinia virus vaccine candidates for Zika virus, which showed high titers in vitro and induced robust immune responses in mice, providing protection against Zika virus infection.
SCIENTIFIC REPORTS
(2021)
Article
Immunology
Ulrike S. Diesterbeck, Henrike P. Ahsendorf, Andre Frenzel, Ahmad Reza Sharifi, Thomas Schirrmann, Claus-Peter Czerny
Summary: A panel of potent neutralizing antibodies offers protection against orthopoxvirus infections. In an in vivo experiment, purified scFv-1.2.2.H9 and IgG1-1.2.2.H9 partially protected against the challenge with VACV, while only one mouse survived in the scFv-Fc-1.2.2.H9 group.
Article
Virology
Rebecca A. Morgis, Kaylyn Haan, Julie M. Schrey, Rosie M. Zimmerman, Adam R. Hersperger
Summary: This study examined the relationship between ectromelia growth factor (ECGF) and vaccinia growth factor (VGF), finding that despite structural differences, both proteins possess the six conserved cysteine residues crucial for EGF function, with ECGF acting as a mitogen in a similar manner as VGF.
Article
Microbiology
Kevin Chiem, Maria M. Lorenzo, Javier Rangel-Moreno, Maria De la Luz Garcia-Hernandez, Jun-Gyu Park, Aitor Nogales, Rafael Blasco, Luis Martinez-Sobrido
Summary: Recombinant vaccinia viruses expressing two reporter genes, fluorescent (Scarlet or GFP) and luciferase (Nluc), provide a valuable tool to study viral infection dynamics in vitro and in vivo, including host-pathogen interactions and tropism of viral infections. This dual reporter-expressing rVV enhances the ability to track viral infections in cultured cells and animal models.
MICROBIOLOGY SPECTRUM
(2021)
Article
Veterinary Sciences
Zeyu Li, Yuanxiu He, Lijuan Ge, Ran Quan, Junzhen Chen, Yue Hu, Ruixue Sa, Jianhua Liu, Duoliang Ran, Qiang Fu, Huijun Shi
Summary: The anticancer agent berbamine (BBM) has been found to inhibit EHV-1 viral infection both in vitro and in vivo. It effectively suppresses viral entry into cells, DNA replication, virion secretion, and reduces the damage caused by the virus to brain and lung tissues. These findings suggest that BBM could be a promising therapeutic approach for controlling EHV-1 infection in equines.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Yanrong Zhou, Qianpeng Wang, Qi Ying, Xiaomei Zhang, Kan Chen, Ting Ye, Gongchu Li
Summary: This study evaluated the cytotoxic effects of oncolytic vaccinia viruses (oncoVV) carrying different lectins on hepatocellular carcinoma (HCC). Results showed that oncoVV-AVL exhibited the highest cytotoxicity, while oncoVV-APL showed no effect. Further research revealed that AVL may promote oncoVV replication through multiple pathways in a cell-dependent manner.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Virology
Ying-Ray Lee, Chia-Ming Chang, Yuan-Chieh Yeh, Chi-Ying E. Huang, Feng-Mao Lin, Juan-Ting Huang, Chang-Chi Hsieh, Jen-Ren Wang, Hsiao-Sheng Liu
Summary: Honeysuckle contains miRNAs that can target multiple viruses, with let-7a identified as capable of suppressing EV71 replication and pathogenesis. Experimental results confirmed the inhibitory effect of honeysuckle on EV71 replication, partly through upregulation of let-7a expression.
Article
Chemistry, Medicinal
Xue Wang, Ningning Zhou, Tingting Liu, Xiaoyuan Jia, Ting Ye, Kan Chen, Gongchu Li
Summary: The study demonstrates that the engineered oncoVV expressing WCL induced higher levels of apoptosis and cytotoxicity in cancer cells, replicated faster, and showed increased antitumor activity in a mouse xenograft tumor model. This suggests that oncoVV-WCL could provide a new avenue for anticancer gene therapy.
Article
Chemistry, Medicinal
Riqing Jiang, Yufeng Qiu, Xiaomei Zhang, Ningning Zhou, Xiaoyuan Jia, Kan Chen, Yanrong Zhou, Ting Ye, Gongchu Li
Summary: The oncolytic vaccinia virus containing Aphrocallistes vastus lectin (oncoVV-AVL) has shown significant anti-tumor effects in hepatocellular carcinoma. The virus activates tumor immunity by upregulating type I interferon expression and enhances replication by inhibiting ISRE mediated viral defense response. Furthermore, the lectin regulates multiple signaling pathways to promote virus replication.
Article
Immunology
Xijing Qian, Bingan Wu, Hailin Tang, Zhenghan Luo, Zhenghao Xu, Songying Ouyang, Xiangliang Li, Jianfeng Xie, Zhigang Yi, Qibin Leng, Yan Liu, Zhongtian Qi, Ping Zhao
Summary: Rifapentine has been found to be an effective inhibitor of yellow fever virus (YFV), interfering with viral entry and replication. It binds to the RNA-dependent RNA polymerase (RdRp) domain of the viral nonstructural protein NS5. In mouse experiments, rifapentine significantly improves survival rate, alleviates clinical signs, and reduces virus load and organ injury. Its antiviral activity is superior to the previously reported YFV inhibitor sofosbuvir.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Microbiology
Kevin Chiem, Aitor Nogales, Maria Lorenzo, Desarey Morales Vasquez, Yan Xiang, Yogesh K. Gupta, Rafael Blasco, Juan Carlos de la Torre, Luis Martinez-Sobrido
Summary: Despite the eradication of smallpox, some orthopoxviruses, such as monkeypox virus (MPXV), remain important human pathogens. Vaccines for smallpox are effective against MPXV, but limited in access. Current antiviral treatments for MPXV are limited to two FDA-approved drugs. Therefore, there is an urgent need to discover novel antivirals for the treatment of MPXV and other potentially zoonotic orthopoxvirus infections. Here, we found 13 compounds that inhibit both VACV and MPXV, derived from two different libraries of compounds known to inhibit various RNA viruses.
MICROBIOLOGY SPECTRUM
(2023)
Article
Virology
Karine Lima Lourenco, Leandro Andrade Chinalia, Lethicia Ribeiro Henriques, Rodrigo Araujo Lima Rodrigues, Flavio Guimaraes da Fonseca
Summary: The immune modulation of VACV in vivo is clade-related and proportional to the strain's virulence upon infection. VACV-WR and GP1V viruses can down-modulate the immune responses in mice, while PSTV virus shows a weaker ability in immune modulation, demonstrating differences in immune stimulatory capacity among different vaccinia viruses.
Article
Cell Biology
Zezhong Liu, Jie Zhou, Wei Xu, Wei Deng, Yanqun Wang, Meiyu Wang, Qian Wang, Ming Hsieh, Jingming Dong, Xinling Wang, Weijin Huang, Lixiao Xing, Miaoling He, Chunlin Tao, Youhua Xie, Yilong Zhang, Youchun Wang, Jincun Zhao, Zhenghong Yuan, Chuan Qin, Shibo Jiang, Lu Lu
Summary: A new STING agonist CF501 has been discovered to enhance the effectiveness of vaccines. CF501-adjuvanted vaccines elicited strong immune responses in mice, rabbits, and rhesus macaques, producing potent neutralizing antibodies against various SARS-CoV-2 variants and mutants. The immunized animals showed long-lasting immune responses and reduced viral load after SARS-CoV-2 challenge.
Article
Immunology
Jiankai Liu, Baoying Huang, Guifan Li, Xianyun Chang, Yafei Liu, Kai Chu, Jialei Hu, Yao Deng, Dandan Zhu, Jingliang Wu, Li Zhang, Meng Wang, Weijin Huang, Hongxing Pan, Wenjie Tan
Summary: This study evaluated the efficacy and safety of a SARS-CoV-2 inactivated vaccine. The results showed that two doses of the vaccine induced a robust immune response and had a good safety profile. The third dose given 28 days after the second dose had limited boosting antibody response.
JOURNAL OF INFECTIOUS DISEASES
(2022)
Article
Chemistry, Multidisciplinary
Ding Yu, Yu-Feng Li, Hong Liang, Jun-Zheng Wu, Yong Hu, Yan Peng, Tao-Jing Li, Ji-Feng Hou, Wei-Jin Huang, Li-Dong Guan, Ren Han, Yan-Tao Xing, Yong Zhang, Jia Liu, Lu Feng, Chun-Yan Li, Xiao-Long Liang, Ya-Ling Ding, Zhi-Jun Zhou, De-Ming Ji, Fei-Fei Wang, Jian-Hong Yu, Kun Deng, Dong-Mei Xia, De-Mei Dong, Heng-Rui Hu, Ya-Jie Liu, Dao-Xing Fu, Yan-Lin He, Dong-Bo Zhou, Hui-Chuan Yang, Rui Jia, Chang-Wen Ke, Tao Du, Yong Xie, Rong Zhou, Ce-Sheng Li, Man-Li Wang, Xiao-Ming Yang
Summary: COVID-HIG, prepared from plasma of Sinopharm COVID-19 vaccine recipients, demonstrates broad-spectrum neutralization effects against various SARS-CoV-2 variants and shows significant prophylactic and treatment efficacy in a mouse model.
Article
Microbiology
Min Zhang, Xinwei Wang, Linhan Hu, Yuting Zhang, Hang Zheng, Haiyan Wu, Jing Wang, Longlong Luo, He Xiao, Chunxia Qiao, Xinying Li, Weijin Huang, Youchun Wang, Jiannan Feng, Guojiang Chen
Summary: In this study, the researchers demonstrated that TIM-1 enhances the cellular entry and infection of Ebola virus, including strains with GP mutations. They also found that recombinant TIM-1 protein can decrease the infectivity of the virus and show synergistic inhibitory effects with a monoclonal antibody. These findings suggest that TIM-1 protein could be a potential therapeutic avenue for Ebola virus and its mutated species.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Fan Zhang, Zhaogeng Yang, Cong Dai, Qiyu He, Zhaochao Liang, Tianxu Liu, Weijin Huang, Youchun Wang, Lin Wang, Ling Wang
Summary: This study demonstrated that the HEV vaccine could induce high titers of protective antibodies against HEV in pregnant rabbits within a short period of time, and these antibodies could be transferred to neonates through the placenta and breast milk, providing protection against HEV infection. This suggests that HEV 239 vaccine could be considered for maternal vaccination.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Virology
Xinjie Li, Yue Yin, Qianqian Cui, Weijin Huang, Qinghua Zou, Tao Shen
Summary: The long-term protective efficacy of neutralizing antibodies (Nabs) against Omicron subvariants after inactivated booster vaccines remains uncertain. This study found that the antibody response against Omicron subvariants was weaker compared to D614G, with BA.4/5 being the least responsive. However, the antibody potency post-booster vaccination was sustained and measurable.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Immunology
Jingjing Liu, Xishan Lu, Xingxing Li, Weijin Huang, Enyue Fang, Wenjuan Li, Xiaohui Liu, Minglei Liu, Jia Li, Ming Li, Zelun Zhang, Haifeng Song, Bo Ying, Yuhua Li
Summary: This study developed an mRNA vaccine using the nucleotide sequence encoding structural proteins of CHIKV, and evaluated its immunogenicity. The encoded proteins elicited high levels of neutralizing antibody titers and T cell-mediated cellular immune responses in mice. Furthermore, a homologous booster mRNA vaccine regimen using different immunization strategies showed higher levels of neutralizing antibody titers and T-cell immune responses.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cell Biology
Yufeng Luo, Shuo Liu, Jiguo Xue, Ye Yang, Junxuan Zhao, Ying Sun, Bolun Wang, Shenyi Yin, Juan Li, Yuchao Xia, Feixiang Ge, Jiqiao Dong, Lvze Guo, Buqing Ye, Weijin Huang, Youchun Wang, Jianzhong Jeff Xi
Summary: This study established a robust mammalian cell-surface-display platform to study the interactions between SARS-CoV-2 variants, cellular receptor ACE2, and monoclonal antibodies on a large scale. By analyzing the mutational landscape, it was found that certain key mutations in the spike protein can increase infectivity and confer resistance to specific monoclonal antibodies. These methods have significant implications for the precise control of SARS-CoV-2 in the future.
Article
Immunology
Xi Wu, Nana Fang, Ziteng Liang, Jianhui Nie, Sen Lang, Changfa Fan, Chunnan Liang, Weijin Huang, Youchun Wang
Summary: Researchers have developed an animal model using SARS-CoV-2 pseudovirus, which is administered intranasally for infection, and bioluminescent imaging allows monitoring of infected tissues and viral load. The model can be used to evaluate the protective efficacy of vaccines and antiviral drugs, as well as the tissue tropism of different viral variants.
Article
Infectious Diseases
Feng-Cai Zhu, Guo-Hua Zhong, Wei-Jin Huang, Kai Chu, Li Zhang, Zhao-Feng Bi, Kong-Xin Zhu, Qi Chen, Ting-Quan Zheng, Ming-Lei Zhang, Sheng Liu, Jin-Bo Xu, Hong-Xing Pan, Guang Sun, Feng-Zhu Zheng, Qiu-Fen Zhang, Xiu-Mei Yi, Si-Jie Zhuang, Shou-Jie Huang, Hui-Rong Pan, Ying-Ying Su, Ting Wu, Jun Zhang, Ning-Shao Xia
Summary: Cecolin 9, an Escherichia coli-produced HPV 9-valent vaccine, was found to be non-inferior in terms of immunogenicity compared to Gardasil 9. It has the potential to contribute to the global accessibility of 9-valent HPV vaccinations, particularly in low-income and middle-income countries.
LANCET INFECTIOUS DISEASES
(2023)
Article
Multidisciplinary Sciences
Yue-Mei Hu, Zhao-Feng Bi, Ya Zheng, Li Zhang, Feng-Zhu Zheng, Kai Chu, Ya-Fei Li, Qi Chen, Jia-Li Quan, Xiao-Wen Hu, Xing-Cheng Huang, Kong-Xin Zhu, Ya-Hui Wang-Jiang, Han -Min Jiang, Xia Zang, Dong-Lin Liu, Chang -Lin Yang, Hong-Xing Pan, Qiu-Fen Zhang, Ying-Ying Su, Shou-Jie Huang, Guang Sun, Wei-Jin Huang, Yue Huang, Ting Wu, Jun Zhang, Ning-Shao Xia
Summary: This study evaluated the immunogenicity and safety of the Escherichia coli-produced second-generation nonavalent HPV vaccine (Cecolin 9). The results showed that Cecolin 9 had good immunogenicity against all nine HPV types and was well tolerated.
Article
Biochemistry & Molecular Biology
Zhen Cui, Wenxin Luo, Ruihong Chen, Yalun Li, Zhoufeng Wang, Yong Liu, Shuo Liu, LeiLei Feng, Zijing Jia, Ruixin Cheng, Jun Tang, Weijin Huang, Yanjun Zhang, Huawen Liu, Xiangxi Wang, Weimin Li
Summary: The emergence of adapted variants of the SARS-CoV-2 virus has led to an increase in breakthrough infections worldwide. Analysis of immune responses in vaccinated individuals reveals limited resistance to Omicron in those with no prior infection but significant immunity in those previously infected. T-cell responses remain largely unaffected, indicating the importance of T-cell-mediated cellular immunity. Administration of a third vaccine dose enhances neutralizing antibodies and memory B cells, improving protection against emerging variants.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Article
Biochemistry & Molecular Biology
Ye Sang, Zhen Zhang, Fan Liu, Haitao Lu, Changxiao Yu, Huisheng Sun, Jinrong Long, Yiming Cao, Jierui Mai, Yiqi Miao, Xin Wang, Jiaxin Fang, Youchun Wang, Weijin Huang, Jing Yang, Shengqi Wang
Summary: Monkeypox has been declared a public health emergency by the World Health Organization. Two mRNA vaccines, mRNA-A-LNP and mRNA-B-LNP, based on specific proteins of the monkeypox virus, have been developed and shown to induce immune responses in mice, including specific antibodies and cellular immunity. These vaccines also provide protection against vaccinia virus challenge in mice and have the potential to be used against other orthopoxviruses.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Correction
Cell Biology
Shuo Liu, Zijing Jia, Jianhui Nie, Ziteng Liang, Jingshu Xie, Lei Wang, Li Zhang, Xiangxi Wang, Youchun Wang, Weijin Huang
Article
Biochemistry & Molecular Biology
Rongzhang He, Xingyu Zheng, Jian Zhang, Bo Liu, Qijie Wang, Qian Wu, Ziyan Liu, Fangfang Chang, Yabin Hu, Ting Xie, Yongchen Liu, Jun Chen, Jing Yang, Shishan Teng, Rui Lu, Dong Pan, You Wang, Liting Peng, Weijin Huang, Velislava Terzieva, Wenpei Liu, Youchun Wang, Yi-Ping Li, Xiaowang Qu
Summary: The study found that spike-specific CXCR3(+) T-FH cells play a crucial role in antibody maintenance and recall responses after SARS-CoV-2 infection and vaccination, exhibiting a more durable response and better ability to induce antibody secretion.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
