Article
Agriculture, Dairy & Animal Science
Arxel G. Elnar, Geun-Bae Kim
Summary: Clostridium perfringens B20 was isolated from chicken feces in Anseong, Korea, and its genome sequence revealed the presence of genes related to immunity and transport.
JOURNAL OF ANIMAL SCIENCE AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Yeweon Choi, Eunsu Ha, Minsuk Kong, Sangryeol Ryu
Summary: Clostridium perfringens is a bacterium that causes diseases in humans and livestock. Antibiotic resistance is a growing concern, necessitating the search for alternative biocontrol agents. In this study, a C. perfringens-infecting bacteriophage and its endolysin were isolated and characterized. Through domain shuffling, a chimeric endolysin was generated with higher antimicrobial activity than the parental endolysin.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Veterinary Sciences
Chake Keerqin, Katherine McGlashan, Thi Thu Hao Van, Helene N. Chinivasagam, Robert J. Moore, Mingan Choct, Shu-Biao Wu
Summary: This study isolated three highly active bacteriophages from Australian commercial poultry environments, which were capable of lysing several disease-causing bacteria. Animal trials showed that oral administration of the bacteriophages cocktail significantly alleviated intestinal necrotic lesions. However, no effect on growth performance was observed.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Multidisciplinary Sciences
Xiu Zeng, Baosheng Liu, Jiao Zhou, Yimin Dai, Chunsheng Han, Linkang Wang, Yunzheng Wu, Jinhua Zhang
Summary: Clostridium perfringens is a common opportunistic pathogen with pathogenicity dependent on toxin production. Analysis of the genome of a piglet-derived strain revealed the presence of two toxin genes, with cpb2 located on a large plasmid.
SCIENTIFIC REPORTS
(2021)
Article
Microbiology
Seung Yeup Lee, Roniya Thapa Magar, Hyo Jeong Kim, Kihyuck Choi, Seon-Woo Lee
Summary: In this study, 72 phages were isolated from various crop cultivated soils in Korea, with phage RpY1 chosen for further investigation due to its specificity for the targeted host. RpY1 was identified as a member of Podoviridae with a head measuring 60-70 nm, a genome size of 43,284 bp, showing no homology to known phages except for DU_RP_II infecting R. solanacearum.
CURRENT MICROBIOLOGY
(2021)
Article
Multidisciplinary Sciences
Chandrasekar Karthika, Nambiraman Malligarjunan, Ravi Jothi, Thirupathi Kasthuri, Rajaiah Alexpandi, Arumugam Veera Ravi, Shunmugiah Karutha Pandian, Shanmugaraj Gowrishankar
Summary: The study explores the potential of phage therapy as an alternative antimicrobial treatment for multidrug-resistant Pseudomonas aeruginosa infection. Two novel virulent phages PSPa and APPa were investigated and shown to have therapeutic potential against P. aeruginosa. Both phages exhibited host specificity and significantly improved the survival rate of bacterial-infected zebrafish in vivo.
SCIENTIFIC REPORTS
(2023)
Article
Veterinary Sciences
Hyun-Gwan Lee, Yoo-Bhin Kim, Sang-Hyeok Lee, Jun-Ok Moon, Jong-Pyo Chae, Yu-Jin Kim, Kyung-Woo Lee
Summary: The study aimed to evaluate the antibacterial effects of bacteriophages (BPs) in broiler chickens challenged with Clostridium perfringens. The results showed that dietary encapsulated or powdered BPs reversed the C. perfringens-induced increase in crypt depth and reduced C. perfringens counts in the cecum. Chickens fed with encapsulated BPs also had higher serum IgA levels.
VETERINARY SCIENCES
(2022)
Review
Microbiology
Jennifer Venhorst, Jos M. B. M. van der Vossen, Valeria Agamennone
Summary: The pathogenic bacteria Clostridioides difficile and Clostridium perfringens pose a major threat to human and animal health. The increasing antibiotic resistance has led to the pursuit of novel strategies to combat these infections, including the use of bacteriophages. However, there are limitations to the use of bacteriophages that need to be considered and further research is necessary to optimize their potential.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Virology
Cong Zhang, Jingzhi Yuan, Changming Guo, Chenling Ge, Xiaoye Wang, Deyuan Wei, Xun Li, Hongbin Si, Chuanhuo Hu
Summary: The study identified a lytic lethal Klebsiella pneumoniae phage and evaluated its anti-infective effects in a murine model of K. pneumoniae infection. Phage Bp5 showed characteristics that significantly reduced colony formation by K. pneumoniae and alleviated lung tissue damage.
Article
Microbiology
Mostafa Y. Abdel-Glil, Prasad Thomas, Joerg Linde, Keith A. Jolley, Dag Harmsen, Lothar H. Wieler, Heinrich Neubauer, Christian Seyboldt
Summary: The study established a publicly available genome-wide multilocus sequence-typing (MLST) scheme for Clostridium perfringens. The cgMLST scheme showed high congruence with the core genome-based single-nucleotide-polymorphism typing and provided greater discrimination than classical MLST methods. The scheme was confirmed to be usable for outbreak analysis and can be used for broad-based and standardized epidemiological studies.
MICROBIOLOGY SPECTRUM
(2021)
Article
Virology
Ivan M. M. Pchelin, Pavel V. V. Tkachev, Daniil V. V. Azarov, Andrey N. N. Gorshkov, Daria O. O. Drachko, Vasily V. V. Zlatogursky, Alexander V. V. Dmitriev, Artemiy E. E. Goncharov
Summary: In the human gut, temperate bacteriophages interact with bacteria through predation and horizontal gene transfer. Metagenomic studies have revealed associations between shifts in phage abundance and various human diseases. A new genus, Vespunovirus, mainly infecting Enterococcus spp., was proposed based on genome network and phylogenomic analyses of the temperate bacteriophage VEsP-1 and related phages identified through database searches and visualization of viral dark matter sequence space.
Article
Agriculture, Dairy & Animal Science
Dongryeoul Bae, Jeong-Woo Lee, Jong-Pyo Chae, Jae-Won Kim, Jong-Su Eun, Kyung-Woo Lee, Kun-Ho Seo
Summary: High incidence and mortality rates of necrotic enteritis in poultry can be caused by the interaction between Clostridium perfringens and Eimeria spp. Banning prophylactic antibiotics in feed has led to increased NE outbreaks, but using CP-specific bacteriophages as a dietary supplement shows promise in controlling NE and improving broiler performance.
Article
Chemistry, Applied
Jae-Hyun Cho, Joon-Gi Kwon, Daniel J. O'Sullivan, Sangryeol Ryu, Ju-Hoon Lee
Summary: Clostridium perfringens, a well-known foodborne pathogen, can be effectively lysed and detected rapidly using endolysin and CBD from bacteriophages. The phage CPAS-15 genome contains an endolysin gene and shows safety in food applications. Both LysCPAS15 and EGFP-LysCPAS15_CBD1 exhibit broader host range and higher stability, providing extended protection from C. perfringens contamination in food.
Article
Microbiology
Anny Camargo, Enzo Guerrero-Araya, Sergio Castaneda, Laura Vega, Maria X. Cardenas-Alvarez, Cesar Rodriguez, Daniel Paredes-Sabja, Juan David Ramirez, Marina Munoz
Summary: In this study, the genetic diversity and phylogenetic relatedness of C. perfringens were assessed using 372 genomes from multiple locations and sources. The results showed that C. perfringens is present in various host species and environments, and exhibits a wide range of virulence factors and antibiotic resistance markers.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Multidisciplinary Sciences
Lidong Dong, Chao Fang, Qun Cheng, Tong Su, Kun Kou, Lingping Kong, Chunbao Zhang, Haiyang Li, Zhihong Hou, Yuhang Zhang, Liyu Chen, Lin Yue, Lingshuang Wang, Kai Wang, Yongli Li, Zhuoran Gan, Xiaohui Yuan, James L. Weller, Sijia Lu, Fanjiang Kong, Baohui Liu
Summary: This study reports on the genes Tof16 and the J locus that control soybean yield under short-day conditions, and the loss of their function contributes to soybean's adaptation to tropical regions. Modulating the genetic complexity of LHY homolog alleles can quantitatively improve soybean's maturity and yield traits.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Applied
Daniela Metro, Mattia Papa, Luigi Manasseri, Teresa Gervasi, Luca Campone, Vito Pellizzeri, Roberta Tardugno, Giacomo Dugo
NATURAL PRODUCT RESEARCH
(2020)
Article
Chemistry, Applied
Abdellah Aghraz, Ambrogina Albergamo, Qada Benameur, Andrea Salvo, Mustapha Larhsini, Mohamed Markouk, Teresa Gervasi, Nicola Cicero
NATURAL PRODUCT RESEARCH
(2020)
Article
Chemistry, Applied
Valentina Mangano, Teresa Gervasi, Archimede Rotondo, Paolo De Pasquale, Giacomo Dugo, Francesco Macri, Andrea Salvo
Summary: This study converts residues from the production of Anchovies into hydrolysate through enzymatic treatment to recover worthy protein-rich fish waste, showing promising biological and nutritional content for the production of high nutritional value commercial products.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Applied
Concetta Calabro, Clara Bertuccio, Teresa Gervasi, Eugenia Rita Lauriano, Michelangelo Leonardi, Nicola Cicero, Patrizia Lo Cascio
Summary: The study found that food additives such as Spirulina can improve fertility in laboratory farmed fish, which may contribute to wider research aimed at improving reproduction in all vertebrates, including humans. The expression of Vtg in zebrafish liver is related to the maturation stages of the ovaries, with a significant increase in its expression observed in the liver during advanced maturation stages.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Applied
Alesci Alessio, S. Pergolizzi, T. Gervasi, M. Aragona, P. Lo Cascio, N. Cicero, E. R. Lauriano
Summary: Alcohol abuse can cause intestinal inflammation, leading to an increase in intestinal mucous cells and circulating macrophages, while treatment with astaxanthin can restore intestinal morphology. Immunohistochemical analysis also showed a reduction in alcohol-induced inflammation with astaxanthin treatment, suggesting its potential in preventing damage associated with excessive alcohol consumption within the gastrointestinal tract.
NATURAL PRODUCT RESEARCH
(2021)
Article
Chemistry, Applied
Roberta Tardugno, Teresa Gervasi, Vincenzo Nava, Gaetano Cammilleri, Vincenzo Ferrantelli, Nicola Cicero
Summary: This study evaluated the nutritional and mineral composition of Diospyros kaki fruits from different regions in Italy, finding high levels of dietary fiber, pectins, and potassium. The total polyphenol content varied greatly, making it suitable for special dietary regimens like hypertension and heart diseases.
NATURAL PRODUCT RESEARCH
(2022)
Review
Biochemistry & Molecular Biology
Teresa Gervasi, Davide Barreca, Giuseppina Lagana, Giuseppina Mandalari
Summary: Long-term consumption of tree nuts has been shown to have positive effects on health, impacting glucose modulation, body weight, cardiovascular risk, inflammation, oxidative stress, cognitive performance, and gut microbiota. Nuts are rich sources of nutrients and phytochemicals, which, combined with a healthy lipid profile, can help prevent chronic diseases, protect against oxidative stress and inflammation, and improve cognitive performance, thereby reducing the impact of aging and neurodegeneration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Archimede Rotondo, Giovanna Loredana La Torre, Teresa Gervasi, Giacomo di Matteo, Mattia Spano, Cinzia Ingallina, Andrea Salvo
Summary: A fast HPLC method with fluorescence detector (FD) was developed for the determination of three tocopherols (TOCs) in milk samples from Modicana cattle breed. The optimized ultrasonic extraction combines an efficient and simple saponification at room temperature and a rapid HPLC quantification of TOCs in milk. The proposed procedure, reducing the experimental complexity, allowed accurate extraction and detection of three TOCs in milk samples from Modicana cattle breed.
Article
Microbiology
Teresa Gervasi, Giovanna Ginestra, Francesca Mancuso, Davide Barreca, Laura De Luca, Giuseppina Mandalari
Summary: This study investigates the antifungal potential of 1-(1H-indol-3-yl) derivatives, previously known as tyrosinase inhibitors, against various strains of Candida and Aspergillus. Results show that compounds 3b, 3c, and 3e exhibit fungicidal activity, with compound 3c also inhibiting microbial tyrosinase by up to 28%. The study suggests the potential use of these derivatives as novel therapeutics for treating fungal infections and enhancing the effectiveness of existing antifungals.
Article
Food Science & Technology
Nicola Cicero, Teresa Gervasi, Alessandra Durazzo, Massimo Lucarini, Antonio Macri, Vincenzo Nava, Filippo Giarratana, Roberta Tardugno, Rossella Vadala, Antonello Santini
Summary: Spices and aromatic herbs have historical significance in human nutrition, being a rich source of bioactive compounds. However, they may also pose biological hazards and contain harmful chemical substances. This study aimed to assess the compliance of various spices and herbs from a non-European country market with EU and WHO regulations through microbiological and trace mineral determination. The results showed that the tested spices were safe, meeting the legal criteria for microbiological parameters and potentially toxic substances.
Article
Chemistry, Physical
Consuelo Celesti, Teresa Gervasi, Nicola Cicero, Salvatore Vincenzo Giofre, Claudia Espro, Elpida Piperopoulos, Bartolo Gabriele, Raffaella Mancuso, Giovanna Lo Vecchio, Daniela Iannazzo
Summary: This study investigated the possibility of enhancing the antibacterial properties of titanium implants by covalently inserting bioactive ammonium salts onto the surface of titanium substrates. The chemically modified samples showed good resistance against bacterial adhesion, particularly when containing long alkyl chains. The results underscored the importance of chemical functionalization in improving the antimicrobial activity of metal surfaces.
Article
Chemistry, Multidisciplinary
Teresa Gervasi, Manuela D'Arrigo, Rossana Rando, Maria Teresa Sciortino, Arianna Carughi, Davide Barreca, Giuseppina Mandalari
Summary: This study found that pistachio polyphenols have antimicrobial effects and can effectively inhibit Listeria monocytogenes, one of the most serious pathogens causing foodborne diseases.
APPLIED SCIENCES-BASEL
(2022)
Article
Nutrition & Dietetics
Giuseppina Mandalari, Teresa Gervasi, Daniel W. Rosenberg, Karen G. Lapsley, David J. Baer
Summary: Nuts are nutrient-dense foods with beneficial effects on chronic diseases, especially cardiovascular health. However, there is limited scientific evidence on their effects on gastrointestinal health. This review summarizes important findings and research perspectives on the impact of nut consumption on gastrointestinal health, including digestion and gut microbiota.
Article
Nutrition & Dietetics
Mariarosaria Ingegneri, Antonella Smeriglio, Rossana Rando, Teresa Gervasi, Maria Pia Tamburello, Giovanna Ginestra, Erminia La Camera, Rosamaria Pennisi, Maria Teresa Sciortino, Giuseppina Mandalari, Domenico Trombetta
Summary: This study investigated the nutritional and polyphenolic profile, as well as the antioxidant, antimicrobial, antiviral, and potential prebiotic effects of blanched almond skin (BS) and blanch water (BW) from three different Sicilian cultivars. The results showed that the total phenols and flavonoids contents were high in BS and BW. BS exhibited antiviral activity against herpes simplex virus 1. It also had high fiber and protein contents and low fat and sugar contents, making it nutritionally valuable. The study indicated that cultivar does not significantly affect the chemical and biological properties of BS and BW.
Editorial Material
Chemistry, Multidisciplinary
Teresa Gervasi
APPLIED SCIENCES-BASEL
(2023)
