Journal
ARCHIVES OF VIROLOGY
Volume 156, Issue 10, Pages 1877-1881Publisher
SPRINGER WIEN
DOI: 10.1007/s00705-011-1065-1
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Funding
- Defense Threat Reduction Agency
- Department of Homeland Security's National Biodefense Analysis and Countermeasures Center (NBACC)
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Monkeypox virus (MPXV) causes a vesiculopustular rash illness resembling smallpox in humans and produces a similar disease in nonhuman primates. To enhance the ability of researchers to study experimental MPXV infections, we inserted a gene encoding green fluorescent protein (GFP) into Monkeypox virus Zaire-79. Wild-type and MPXV-GFP replicated with similar kinetics in cell culture and caused a similar disease when injected intravenously into cynomolgus macaques. In MPXV-GFP-infected animals, examination under fluorescent light facilitated the identification of skin lesions during disease development and internal sites of replication at necropsy. MPXV-GFP could improve the quantitative assessment of antiviral therapy and vaccine efficacy.
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