Article
Public, Environmental & Occupational Health
Yu Li, Fengxuan Wang, Ruici Kan, Hui Cao, Cheng Tang, Hua Yue, Bin Zhang
Summary: This study successfully isolated and obtained the genomic sequences of two genotypes of porcine G9 rotaviruses (RVAs), G9P[23] and G9P[13]. The genomic segments of these RVAs were found to be closely related to porcine and/or porcine-like human RVAs, indicating that they are porcine-human reassortant strains. In mouse experiments, suckling mice born to vaccinated dams were protected against challenge with homologous strains, demonstrating the immunogenicity of porcine G9 RVAs.
ZOONOSES AND PUBLIC HEALTH
(2022)
Article
Veterinary Sciences
Ran Tao, Xinjian Chang, Jinzhu Zhou, Xuejiao Zhu, Shanshan Yang, Kemang Li, Laqiang Gu, Xuehan Zhang, Bin Li
Summary: Multiple genotypes of porcine RVA are circulating in pig farms in East China, posing a serious threat to the breeding industry. Continuous surveillance of RVA prevalence in pigs is necessary to support the rational application of vaccines or other measures for prevention and control.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Review
Infectious Diseases
Cornelius A. Omatola, Ademola O. Olaniran
Summary: This review discusses the genetic and antigenic heterogeneity of rotavirus, the mechanism of genetic diversity and evolution, and the implications of genetic changes on vaccination. Evidence suggests that genetic changes in rotavirus can alter its antigenic landscape and enhance its fitness in a fully vaccinated population. Therefore, continued and increased monitoring of variants across the globe is recommended to predict and respond to the emergence of new strains.
EXPERT REVIEW OF ANTI-INFECTIVE THERAPY
(2022)
Article
Virology
Fuxian Zhang, Diangang Sun, Qin Fang
Summary: This study investigated the molecular characteristics of outer capsid proteins VP5 and VP7 of grass carp reovirus (GCRV). New posttranslational modifications were identified in VP5, and the association between VP5 and VP7 was revealed, providing insights into the viral life cycle.
Article
Virology
Gyu-Nam Park, Da In Kim, SeEun Choe, Jihye Shin, Byung-Hyun An, Ki-Sun Kim, Bang-Hun Hyun, Jong-Soo Lee, Dong-Jun An
Summary: This study investigated the prevalence and genetic characteristics of porcine group A rotavirus (PoRVA) in domestic pig farms in South Korea. Multiple predominant circulating genotypes were identified, providing valuable epidemiological information for the development of vaccines targeting the current strains.
Article
Virology
Phat Xuan Dinh, Hai Ngoc Nguyen, Danh Cong Lai, Toan Tat Nguyen, Nam Minh Nguyen, Duy Tien Do
Summary: This study investigated the genetic diversity of PCV3 strains circulating in Vietnam. Out of 249 samples, 11.65% (29/249) were found to contain PCV3. The strains belonged to PCV3b, c, and a subtypes, and showed amino acid substitutions in the capsid proteins.
ARCHIVES OF VIROLOGY
(2023)
Article
Biochemical Research Methods
Evgeny Faizuloev, Ramil Mintaev, Olga Petrusha, Anna Marova, Daria Smirnova, Yulia Ammour, Elena Meskina, Oleg Sergeev, Sergey Zhavoronok, Alexander Karaulov, Oxana Svitich, Vitaly Zverev
Summary: This study introduces a specific approach for nanopore sequencing of rotavirus genomes, allowing accurate sequencing and analysis of viral genome segments. Experimental validation showed the method's ability to obtain full-length sequences of RVA gene segments with high accuracy, making it a promising tool for viral epidemiological studies and surveillance.
JOURNAL OF VIROLOGICAL METHODS
(2021)
Article
Multidisciplinary Sciences
Marie C. Henniges, Robyn F. Powell, Sahr Mian, Clive A. Stace, Kevin J. Walker, Richard J. Gornall, Maarten J. M. Christenhusz, Max R. Brown, Alex D. Twyford, Peter M. Hollingsworth, Laura Jones, Natasha de Vere, Alexandre Antonelli, Andrew R. Leitch, Ilia J. Leitch
Summary: This paper presents the first comprehensive data repository of native and alien vascular plant species in Britain and Ireland, providing fast and easy online access for ecological, evolutionary and conservation analyses. The resource includes a large amount of species and trait information, facilitating fundamental and applied research and enhancing our understanding of plant composition and temporal changes to inform conservation efforts in the face of ongoing climate change and biodiversity loss.
Article
Multidisciplinary Sciences
Joseph J. Malakalinga, Gerald Misinzo, George M. Msalya, Mariana J. Shayo, Rudovick R. Kazwala
Summary: This study assessed the genetic diversity and genomic profiles of rotaviruses in Tanzanian piglets, revealing multiple circulating genotypes and the possibility of intra-genogroup reassortment and interspecies transmission. The findings highlight the importance of genomic analysis and continuous monitoring of rotavirus genotypes.
Article
Veterinary Sciences
Xiao Liu, Chunlian Song, Yinghua Liu, Kaixing Qu, Junyu Bi, Junlong Bi, Yunhua Wang, Ying Yang, Junhua Sun, Zhigang Guo, Ganwu Li, Jianping Liu, Gefen Yin
Summary: This study investigated the prevalence and genetic diversity of porcine sapovirus (PoSaV) infections in Yunnan province, China. The results showed a high infection rate and identified multiple genotypes of PoSaV strains, including the first identification of a PoSaV-GV strain in China. The findings provide important insights for the control of PoSaV infections in the region.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Microbiology
Lujia Zhou, Yang Yang, Qiqi Xia, Zhixin Guan, Junjie Zhang, Beibei Li, Yafeng Qiu, Ke Liu, Donghua Shao, Zhiyong Ma, Xiaodu Wang, Jianchao Wei
Summary: PRRS is an immunosuppressive disease in pigs caused by PRRSV, leading to reproductive and respiratory disorders. The predominant strain in Eastern China is still highly pathogenic PRRSV, with an increasing proportion of NADC30-like and NADC34-like strains. This study enriches the epidemiological data and provides a theoretical basis for the development of vaccines and prevention and control of the disease.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Veterinary Sciences
Amina Nawal Bahoussi, Pei-Hua Wang, Pir Tariq Shah, Hongli Bu, Changxin Wu, Li Xing
Summary: The phylogenetic classification and evolutionary characteristics of Porcine deltacoronavirus (PDCoV) were re-examined in this study. Two major genogroups (GI and GII) and multiple sub-genogroups were identified. PDCoV strains collected in China showed the highest genetic diversity, and several potential recombination events were detected. The spike glycoprotein of PDCoV exhibited significant antigenic drift, suggesting its role in genetic diversity and wider spread of the virus.
BMC VETERINARY RESEARCH
(2022)
Article
Infectious Diseases
Fangfeng Yuan, Yuan Feng, Jianfa Bai, Xuming Liu, Bailey Arruda, Srivishnupriya Anbalagan, Lalitha Peddireddi
Summary: APPV is a highly divergent pestivirus widely distributed around the world, known to cause congenital tremors in piglets. This study revealed a 19.0% prevalence of APPV in US swine herds with significant sequence diversity. The prevalence varied among different US regions, with Minnesota having the highest prevalence at 37.35%, followed by Illinois, Iowa, and Kansas.
TRANSBOUNDARY AND EMERGING DISEASES
(2022)
Article
Virology
Yongsheng Xie, Xiaoru Wang, Junsen Feng, Liuming Wei, Gen Li, Guangbin Si, Yibo Chen, He Yan, Dongsheng He
Summary: The study revealed a widespread presence of PPgV in swine herds in Guangdong Province, with an increasing annual prevalence rate. Sequence analysis showed genetic diversity of PPgV at both nucleotide and amino acid levels, with high genetic similarity to samples from the USA.
Article
Mathematics
Nabeel Al-Milli, Amjad Hudaib, Nadim Obeid
Summary: In this paper, a novel feature selection algorithm Population Diversity Controller-GA (PDC-GA) is proposed, which combines GA with k-mean clustering to enhance the performance of machine learning classifiers, showing an improvement in the range of 1% to 4%.
Article
Veterinary Sciences
Kwonil Jung, Ayako Miyazaki, Linda J. Saif
RESEARCH IN VETERINARY SCIENCE
(2018)
Article
Virology
Anastasia N. Vlasova, Sayaka Takanashi, Ayako Miyazaki, Gireesh Rajashekara, Linda J. Saif
CURRENT OPINION IN VIROLOGY
(2019)
Article
Cell Biology
Hikaru Iida, Masanori Tohno, Md Aminul Islam, Nana Sato, Hisakazu Kobayashi, Leonardo Albarracin, A. K. M. Humayun Kober, Wakako Ikeda-Ohtsubo, Yoshihito Suda, Hisashi Aso, Tomonori Nochi, Ayako Miyazaki, Hirohide Uenishi, Noriyuki Iwabuchi, Jin-zhong Xiao, Julio Villena, Haruki Kitazawa
Article
Immunology
Husheem Michael, Stephanie N. Langel, Ayako Miyazaki, Francine C. Paim, Juliet Chepngeno, Moyasar A. Alhamo, David D. Fischer, Vishal Srivastava, Dipak Kathayat, Loic Deblais, Gireesh Rajashekara, Linda J. Saif, Anastasia N. Vlasova
FRONTIERS IN IMMUNOLOGY
(2020)
Article
Microbiology
V Srivastava, L. Deblais, H-C Huang, A. Miyazaki, S. Kandasamy, S. N. Langel, F. C. Paim, J. Chepngeno, D. Kathayat, A. N. Vlasova, L. J. Saif, G. Rajashekara
BENEFICIAL MICROBES
(2020)
Article
Multidisciplinary Sciences
Husheem Michael, Francine C. Paim, Ayako Miyazaki, Stephanie N. Langel, David D. Fischer, Juliet Chepngeno, Steven D. Goodman, Gireesh Rajashekara, Linda J. Saif, Anastasia Nickolaevna Vlasova
Summary: EcN biofilm treatment enhanced innate and B cell immune responses after HRV infection and ameliorated diarrhea following HRV challenge in a malnourished, HIFM pig model. Increased frequencies of activated plasmacytoid dendritic cells (pDC) and conventional dendritic cells (cDC) were observed, along with increased numbers of systemic and intestinal HRV-specific IgA antibody secreting cells (ASCs) and antibody titers.
Article
Microbiology
Husheem Michael, Francine C. Paim, Stephanie N. Langel, Ayako Miyazaki, David D. Fischer, Juliet Chepngeno, Joshua Amimo, Loic Deblais, Gireesh Rajashekara, Linda J. Saif, Anastasia N. Vlasova
Summary: This study investigated the immune-modulating effects of EcN on Cipro-treated Gn piglets challenged with VirHRV. The results showed that EcN treatment enhanced immune responses, reduced antibody titers, increased immunoglobulin-secreting cells, and altered cytokine levels. The findings suggest that EcN supplementation may be beneficial in alleviating RV-induced diarrhea and modulating immune parameters in neonatal Gn pigs colonized with DM.
Article
Immunology
Yuhki Indo, Shugo Kitahara, Mikado Tomokiyo, Shota Araki, Md Aminul Islam, Binghui Zhou, Leonardo Albarracin, Ayako Miyazaki, Wakako Ikeda-Ohtsubo, Tomonori Nochi, Takato Takenouchi, Hirohide Uenishi, Hisashi Aso, Hideki Takahashi, Shoichiro Kurata, Julio Villena, Haruki Kitazawa
Summary: The study identified L. salivarius strains FFIG35 and FFIG58 with potent immunomodulatory effects, enhancing resistance to rotavirus infection in PIE cells and regulating immune responses in superinfection models. These strains modulated negative regulators of the TLR signaling pathway in epithelial cells, highlighting their potential to be used for developing functional feeds to improve immune health and reduce intestinal infections in weaned piglets.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Husheem Michael, Ayako Miyazaki, Stephanie N. Langel, Joshua O. Amimo, Maryssa K. Kick, Juliet Chepngeno, Francine C. Paim, David D. Fischer, Gireesh Rajashekara, Linda J. Saif, Anastasia N. Vlasova
Summary: This study evaluates the effects of colonization with Escherichia coli Nissle and Lacticaseibacillus rhamnosus GG probiotics on the efficacy of oral rotavirus vaccine, and finds that colonization with Escherichia coli Nissle enhances the immunogenicity and protective efficacy to a greater extent than Lacticaseibacillus rhamnosus GG.
Article
Veterinary Sciences
Takato Takenouchi, Kentaro Masujin, Ayako Miyazaki, Shunichi Suzuki, Michihiro Takagi, Takehiro Kokuho, Hirohide Uenishi
Summary: Macrophages are a crucial part of the immune system and are used in vitro cultures for studying their functions and interactions with pathogens. This study focuses on porcine macrophages and their potential use in identifying and characterizing viral pathogens in pigs. The study successfully isolates and immortalizes porcine intestinal macrophages (PIM) and demonstrates their susceptibility to infection by African swine fever virus and porcine reproductive and respiratory syndrome virus. The developed IPIM cell line can be a valuable tool for studying the relationship between porcine pathogens and immune cells.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Veterinary Sciences
Asuka Kumagai, Sayo Kajikawa, Ayako Miyazaki, Shinichi Hatama
Summary: Bovine adenovirus type 7 (BAdV-7) is a significant pathogen in the cattle industry. The genomic analysis of the attenuated strain TS-GT revealed a high similarity to the pathogenic strain Fukuroi, with a novel mutation and four indels.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2022)
Article
Veterinary Sciences
Akiho Katayama, Ayako Miyazaki, Naohito Okazaki, Teruko Nakayama, Osamu Mikami
Summary: In May 2019, a total of ten 1-2-year-old rabbits died within 2 weeks, showing symptoms such as liver discoloration and fragility, necrotizing hepatitis, fibrin thrombi in small vessels, and hemorrhage. Rabbit hemorrhagic disease (RHD) caused by the global emerging GI.2/RHDV2 was confirmed for the first time in Japan, with the virus gene detected in liver samples and viral particles found in degenerated hepatocytes. Phylogenetic analysis classified the virus as Lagovirus europaeus GI.2/RHDV2.
JOURNAL OF VETERINARY MEDICAL SCIENCE
(2021)
Article
Microbiology
Asuka Kumagai, Sayo Kajikawa, Ayako Miyazaki, Shinichi Hatama
Summary: Researchers determined the complete genome sequence of bovine adenovirus type 7 strain Fukuroi using next-generation sequencing, revealing a 30,034 bp long genome with the shortest inverted terminal repeats among known adenoviruses.
MICROBIOLOGY RESOURCE ANNOUNCEMENTS
(2021)
Article
Veterinary Sciences
Yuto Suda, Ayako Miyazaki, Kohtaro Miyazawa, Tomoyuki Shibahara, Seiichi Ohashi
Summary: Porcine epidemic diarrhea (PED) is a coronavirus disease that causes severe diarrhea to spread rapidly among pigs. Research has shown fluctuations in PEDV IgA antibody levels in different parts of the intestine, with IgA-secreting cells mainly located in the upper jejunum and memory B cells enhancing antibody production in the mesenteric lymph nodes and ileum. These findings will aid in the development of effective vaccines and diagnostic methods for PEDV.
VETERINARY RESEARCH
(2021)
Article
Agricultural Engineering
T. Naide, A. Ikeguchi, A. Miyazaki, K. Katsuda
TRANSACTIONS OF THE ASABE
(2018)