Article
Biochemical Research Methods
Mari-Luiza Konjaria, Rusudan Kakava, Alessandro Volonterio, Bezhan Chankvetadze, Gerhard K. E. Scriba
Summary: This study evaluated the enantioseparation of 16 chiral compounds using capillary electrophoresis with charged cyclodextrins as chiral selectors. It found that randomly substituted cyclodextrins showed good separation ability for the model analytes. The study also discovered the influence of complexation constants and complex mobilities on the migration order of enantiomers.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Sulaiman Krait, Antonio Salgado, Milo Malanga, Tamas Sohajda, Gabor Benkovics, Peter Soma Szakaly, Bezhan Chankvetadze, Gerhard K. E. Scriba
Summary: This study investigated the separation of daclatasvir and its R,R,R,R-enantiomer using capillary electrophoresis with various chiral selectors. The type and composition of derivatized CDs were found to be crucial for chiral separations, with the content of 2,6-DM-β-CD significantly impacting the migration order of enantiomers.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Biochemical Research Methods
Mustafa Alawadi, Ali Reza Fakhari, Mohammad Reza Bayatloo, Saeed Nojavan
Summary: In this study, carboxymethylated maltodextrin (Cm-MD) was synthesized as an efficient anionic chiral selector and used for enantiomer separation of basic drugs by capillary electrophoresis. Cm-MD modified CE method achieved successful enantiomer separation with higher resolution compared to MD modified CE.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Multidisciplinary
Shirong Cao, Qianyun Ma, Ye Liu, Jiayu Zhang, Zhaokun Wang
Summary: In this study, direct chiral separation of ofloxacin enantiomers using a Chiral MD (2) column as a chiral stationary phase (CSP) by normal phase HPLC method was achieved, and the method was applied to the quality control of two commercial pharmaceuticals containing ofloxacin racemate and S-enantiomer, respectively. The results showed that the addition of acid and alkaline additives to the mobile phase simultaneously was crucial for improving the enantiomeric resolution. Molecular docking research revealed that the binding energy difference between each enantiomer and CSP was maximized when the ofloxacin molecule was non-ionized, explaining the necessity of both acid and alkaline additives in the enantioseparation. Computer simulation results further demonstrated the involvement of hydrogen bonding, hydrophobic effects, and pi-pi stacking in the stereoselective interaction, leading to the chiral separation of ofloxacin enantiomers.
NEW JOURNAL OF CHEMISTRY
(2022)
Article
Biochemical Research Methods
Zuo La, Cecile Danel, Gaelle Grolaux, Julie Charton, Christophe Furman, Emmanuelle Lipka
Summary: A capillary electrokinetic chromatography method has been developed for sterioisomeric separation of a neutral, hydrophobic compound with multiple chiral centers, successfully separating eight isomers. The background electrolyte containing three cyclodextrins was found to be crucial for the separation process.
Article
Biochemistry & Molecular Biology
Ida Fejos, Eszter Kalydi, Edit Luca Kukk, Mimimorena Seggio, Milo Malanga, Szabolcs Beni
Summary: This study investigated the chiral recognition mechanisms of positively charged cyclodextrin derivatives through synthesis, pH determination, and chiral capillary electrophoretic studies. The results indicated that these derivatives can be effective chiral selectors for discriminating between various model racemates. The study also found that substituent-dependent enantiomer migration order reversal occurred in certain cyclodextrins.
Article
Chemistry, Physical
Ang Li, Siru Ren, Chuanjiang Teng, Hongmei Liu, Qi Zhang
Summary: Deep eutectic solvents (DESs) have potential applications as additives/modifiers in chiral electrokinetic chromatography (EKC) and can moderately improve the enantioseparation performance. The improvement is mainly attributed to the contribution of hydrogen-bond acceptors (HBAs) rather than hydrogen-bond donors (HBDs). However, using chiral DESs does not show obvious advantages compared to achiral DESs, which is different from the performance of chiral ionic liquids (ILs) as additives in chiral EKC.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Multidisciplinary Sciences
Melania Carcu-Dobrin, Gabriel Hancu, Lajos Attila Papp, Ibolya Fulop, Hajnal Kelemen
Summary: Chirality, a property of asymmetry, plays a crucial role in determining the pharmacokinetic and pharmacological profiles of optically active pharmaceuticals. A new chiral separation method for VER enantiomers by CE using CDs was successfully developed, achieving efficient enantiomeric separation and optimization through experimental design.
Article
Biochemical Research Methods
Katerina A. Ioannou, Atalanti Christou, Ioannis J. Stavrou, Martin G. Schmid, Constantina P. Kapnissi-Christodoulou
Summary: A simple and easy-to-prepare electrophoretic method was developed for the enantioseparation of amphetamine and cathinone derivatives in this study. Different types of chiral selectors were utilized, with the most effective ones identified for optimal resolution and analysis time.
Article
Chemistry, Analytical
Chengchen Zhang, Xiaofei Ma
Summary: A synergistic system for enantioseparation in capillary electrophoresis (CE) with a chiral ionic liquid (CIL) based on D-10-camphorsulfonic acid as an additive and carboxymethyl-beta-cyclodextrin (CM-beta-CD) as the chiral selector is presented in this work. The proposed method showed excellent enantioseparation performance towards sixteen chiral drugs, with notably improved resolution (Rs) and selectivity factor (alpha) compared to the single CM-beta-CD system. Several key parameters were investigated, and Statistical Product and Service Solutions (SPSS) was used to prove the potential synergistic effect. The nuclear magnetic resonance (NMR) results further demonstrated the function of the CIL and the superiority of the synergistic system. Chiral impurity determination of chlorpheniramine maleate sample was successfully carried out using the established method.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Review
Biochemistry & Molecular Biology
Gabriel Hancu, Lajos Attila Papp, Gergo Toth, Hajnal Kelemen
Summary: The use of dual CD systems in pharmaceutical analysis enhances enantioseparation selectivity by simultaneous interaction with two chiral selectors. The review explains theoretical aspects of enantiomer separation and highlights the advantages, disadvantages, potential, and new directions in this chiral analysis field.
Article
Chemistry, Analytical
Yiqun Li, Lin Zhao, Jiayi Li, Shanshan Xie, Ning Liang
Summary: A gamma-Cyclodextrin based metal-organic framework with D-Histidine as a chiral ligand was synthesized for the selective enrichment of S-Naproxen enantiomer. Computer simulations predicted intermolecular binding patterns and the difference in affinity due to the differentiation in pi-lone pair miscellaneous and hydrogen bonding force capacities. This chiral adsorption material shows great potential as a chiral resolution agent.
MICROCHEMICAL JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Daniel F. Kawano, Bruna Z. Costa, Katherine L. Romero-Orejon, Hugo C. Loureiro, Dosil P. de Jesus, Anita J. Marsaioli
Summary: In this study, a 5-hexyl-2-methyl-3,4-dihydro-2H-pyrrole was synthesized and enantiomers were successfully separated using capillary electrophoresis. The experiment demonstrated that one enantiomer exhibited higher affinity for the chiral selector.
Article
Chemistry, Analytical
Adriana Modroiu, Sulaiman Krait, Gabriel Hancu, Gerhard K. E. Scriba
Summary: A capillary electrophoresis method for the analysis of silodosin drug substance and pharmaceutical formulations was developed to control the enantiomeric purity. Method optimization was performed using a quality by design strategy and the method was validated according to international guidelines. The method was successfully applied to the analysis of a commercial capsule formulation.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Xuebo Zhang, Jing Xu, Zhaofeng Sun, Guangling Bian, Ling Song
Summary: A new boric agent, boric acid D, with a bridged structure has been synthesized and used as a highly efficient chiral derivative agent for distinguishing between various diols. The derivatization reaction is fast and complete, easy to operate, and provides accurate measurements of enantiomeric excess (ee) values. The characteristic split NMR signals are well-distinguishable with a large chemical shift difference (up to 0.39 ppm).
Article
Chemistry, Analytical
Xuan-Lan Mai, Thuy-Vy Pham, Thi-Anh-Tuyet Le, Bao-Tan Nguyen, Ngoc Van Thi Nguyen, Jong-Seong Kang, Woongchon Mar, Kyeong Ho Kim
JOURNAL OF SEPARATION SCIENCE
(2020)
Article
Plant Sciences
Dan Gao, Chong Woon Cho, Cheong Taek Kim, Won Seok Jeong, Jong Seong Kang
Article
Chemistry, Analytical
Bao-Tan Nguyen, Thi-Anh-Tuyet Le, Xuan-Lan Mai, Thi Ngoc Van Nguyen, Thanh Dung Phan, Jong-Seong Kang, Kyeong Ho Kim
Summary: A high-performance liquid chromatography method was developed to determine the enantiomeric impurity of tenofovir disoproxil fumarate, which successfully separated the main component from the minor enantiomeric impurity, and was validated for the quantification of enantiomeric impurity in raw materials.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Hyung Min Kim, Jong Seong Kang
Summary: Environmental pollution has significant toxicity to ecosystems. Environmental metabolomics is a powerful technique for studying the effects of pollutants. This review covers the entire process from sample preparation to data interpretation and discusses model organisms and emerging environmental toxicants.
Article
Plant Sciences
Jin Hyeok Kim, Dan Gao, Chong Woon Cho, Inkyu Hwang, Hyung Min Kim, Jong Seong Kang
Summary: In this study, a bioanalytical method using LC-MS/MS was developed and applied to a pharmacokinetic study of inotodiol, a substance known for its anti-cancer activity. The method was validated with high accuracy and precision, and the pharmacokinetic parameters after intravenous and oral administration were calculated. The absolute oral bioavailability of inotodiol was found to be 0.45%, similar to nonpolar phytosterols. This study represents the first bioanalytical method and pharmacokinetic investigation of inotodiol.
Article
Pharmacology & Pharmacy
Kim-Ngan Huynh Nguyen, Ngoc-Van Thi Nguyen, Kyeong Ho Kim
Summary: Physalis angulata L. is a wild plant commonly used in traditional Vietnamese medicine. A study quantified seven major compounds in different parts of the plant, demonstrating a validated method for analyzing active compounds. This method can serve as a useful tool for quality control of Physalis angulata.
Editorial Material
Plant Sciences
Jong-Seong Kang, Narendra Singh Yadav
Article
Biochemistry & Molecular Biology
Jang Hoon Kim, Chong Woon Cho, Mok Hur, Woo Tae Park, Youn-Ho Moon, Sung-Cheol Koo, Yun-Chan Hur, Jong Seong Kang, Ik Soo Lee
Summary: The quaternary isoquinoline alkaloids palmatine, berberine, and jatrorrhizine were found to have similar inhibitory activities against soluble epoxide hydrolase (sEH), binding noncompetitively with the enzyme and inhibiting its catalytic reaction. Computational chemistry analysis revealed the potential binding site of these alkaloids to the receptor. They are considered suitable lead compounds for the development of sEH inhibitors.
CURRENT ISSUES IN MOLECULAR BIOLOGY
(2022)
Article
Chemistry, Applied
Linh Tuyen Thi Nguyen, Truc Trung Tran, Ngoc-Van Thi Nguyen
Summary: This study utilized spray drying method to prepare dried extracts from Rehmannia glutinosa root and Coix lachryma-jobi trunk. By optimizing the extraction conditions and drying parameters, low moisture content extracts were obtained. This method has the potential to reduce research funds and the price of dried extracts.
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2022)
Article
Chemistry, Applied
Ngoc-Van Thi Nguyen, Ngan Tuyet Duong, Kim-Ngan Huynh Nguyen, Nguyen Thao Bui, To-Lien Thi Pham, Kien Trung Nguyen, Phuoc Huu Le, Kyeong-Ho Kim
Summary: This study optimized the extraction process to obtain high total phenolic and flavonoid contents from Avicennia officinalis L's leaves using solvents with different polarities. The results showed that methanol had the highest extraction yield, acetone exhibited the highest antioxidant activity in the DPPH assay, and ethyl acetate showed the highest antioxidant activity in the ABTS+ assay. The acetone extract also had high phenolic and flavonoid contents.
BIOINTERFACE RESEARCH IN APPLIED CHEMISTRY
(2022)
Article
Spectroscopy
Thuy-Vy Pham, Gunhee Lee, Xuan-Lan Mai, Thi-Anh-Tuyet Le, Thi Ngoc Van Nguyen, Jongki Hong, Kyeong Ho Kim
Summary: A simple, specific, and economical LC-MS/MS method was developed for screening a variety of antihypertensive and related drugs in human urine. The method showed good specificity, reproducibility, and sensitivity, with a wide linear range for analytes. The developed method improved on currently existing methods by enhancing sensitivity and expanding coverage.
MASS SPECTROMETRY LETTERS
(2021)
Article
Food Science & Technology
N. V. T. Nguyen, K. N. H. Nguyen, K. T. T. Dam, H. T. T. Vo, K. A. T. Nguyen, K. H. Kim
Summary: A simple and inexpensive liquid chromatography method was developed for the analysis of food dyes in a variety of food products. The method utilized a reverse stationary phase for the separation of 11 water-soluble dyes and was successfully applied to the quantitative analysis of 36 commercial products.
INTERNATIONAL FOOD RESEARCH JOURNAL
(2021)
Article
Chemistry, Analytical
Xuan-Lan Mai, Yusung Choi, Quoc-Ky Truong, Thi-Ngoc-Van Nguyen, Sang Beom Han, Kyeong Ho Kim
ANALYTICAL SCIENCE AND TECHNOLOGY
(2020)
Article
Chemistry, Analytical
Seung-Beom Lee, Thuy-Vy Pham, Xuan-Lan Mai, Thi-Anh-Tuyet Le, Thi-Ngoc-Van Nguyen, Jong-Seong Kang, Woongchon Mar, Kyeong Ho Kim
ANALYTICAL SCIENCE AND TECHNOLOGY
(2020)
Article
Chemistry, Analytical
Yusung Choi, Thuy-Vy Pham, Xuan-Lan Mai, Quoc-Ky Truong, Thi-Anh-Tuyet Le, Thi-Ngoc-Van Nguyen, Gunhee Lee, Jong-Seong Kang, Woongchon Mar, Kyeong Ho Kim
ANALYTICAL SCIENCE AND TECHNOLOGY
(2019)