Article
Multidisciplinary Sciences
Maki Kiso, Yuri Furusawa, Ryuta Uraki, Masaki Imai, Seiya Yamayoshi, Yoshihiro Kawaoka
Summary: In this study, the authors characterized mutant viruses with reduced sensitivity to nirmatrelvir, an oral antiviral agent that targets SARS-CoV-2. They found that these mutant viruses showed a slower growth rate and attenuated phenotypes in infection models. However, it is important to closely monitor the emergence of nirmatrelvir-resistant SARS-CoV-2 variants as they could potentially become dominant.
NATURE COMMUNICATIONS
(2023)
Article
Multidisciplinary Sciences
Nader S. Abutaleb, Ahmed E. M. Elhassanny, Daniel P. Flaherty, Mohamed N. Seleem
Summary: Dorzolamide demonstrates potent activity against clinical VRE isolates and shows synergistic and additive interactions with gentamicin. In an in vivo mouse model, dorzolamide outperforms linezolid in reducing VRE colonization, suggesting it as a promising treatment option for VRE infections.
Article
Infectious Diseases
Cristina Lagatolla, Jai W. Mehat, Roberto Marcello La Ragione, Roberto Luzzati, Stefano Di Bella
Summary: The combination of oritavancin and fosfomycin showed synergistic effect and increased drug susceptibility against vancomycin-resistant enterococci. It also showed promising results in reducing bacterial regrowth and restoring fosfomycin susceptibility.
Article
Infectious Diseases
Mattia Pirolo, Mareliza Menezes, Peter Damborg, Alice Wegener, Birgitta Duim, Els Broens, Lisbeth Rem Jessen, Mette Schjaerff, Luca Guardabassi
Summary: The study found a correlation between oxacillin MICs and PBP mutations in MRSP strains, as well as their susceptibility to beta-lactams approved for veterinary use. Strains with low-level MRSP had an oxacillin MIC < 4 mg/L and were susceptible to cefalexin, while being less susceptible to amoxicillin/clavulanate. High oxacillin MICs were associated with mutations in multiple PBPs.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Valeria Allizond, Lorenza Cavallo, Janira Roana, Narcisa Mandras, Anna Maria Cuffini, Vivian Tullio, Giuliana Banche
Summary: This study evaluated the antifungal activity of eleven commercial essential oils against clinical isolates of Aspergillus species. The results demonstrated that lemongrass essential oil exhibited the strongest antimicrobial activity, while clove and geranium essential oils were fairly effective in inhibiting Aspergillus spp. growth.
Article
Microbiology
Hazim O. Khalifa, Hidetaka Majima, Akira Watanabe, Katsuhiko Kamei
Summary: This study analyzed the interaction of 21 antifungal combinations with C. glabrata isolates, finding that most caspofungin combinations with azoles or amphotericin B showed synergistic interactions, while azole and 5-flucytosine combinations with azoles or amphotericin B did not. None of the combinations showed antagonistic interactions, with most of the detected synergistic actions occurring among echinocandin-resistant isolates.
Article
Microbiology
Amel Saim-Mamoun, Yacine Abed, Julie Carbonneau, Guy Boivin
Summary: This study demonstrated the importance of the I38T PA substitution in conferring resistance to baloxavir marboxil (BXM) in influenza B viruses. The I38T substitution significantly increased the BXM IC50 value and reduced viral titers, suggesting its impact on the fitness of influenza variants. However, the mutation had no effect on polymerase activity and remained stable over passages.
Article
Infectious Diseases
Seongman Bae, Eun Sil Kim, Yun Woo Lee, Jiwon Jung, Min Jae Kim, Yong Pil Chong, Sung-Han Kim, Sang-Ho Choi, Sang-Oh Lee, Yang Soo Kim
Summary: This study evaluated the clinical characteristics of rifampicin-resistant Staphylococcus aureus (MRSA) infections and found a significant association between rifampicin resistance and infection recurrence.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Environmental Sciences
Anna Lawniczek-Walczyk, Marcin Cyprowski, Rafal L. Gorny
Summary: This study aimed to assess the prevalence of enterococci strains (including vancomycin-resistant) in meat plants and found that enterococci were present in more than 72% of the tested samples. In addition, more than 25% of the tested samples contained vancomycin-resistant enterococci strains, and they showed potential for biofilm formation.
ROCZNIK OCHRONA SRODOWISKA
(2022)
Article
Immunology
Sujin Oh, Soo Kyung Nam, Ho Eun Chang, Kyoung Un Park
Summary: In this study, we performed whole-genome sequencing of vancomycin-resistant enterococci (VRE) using both short-read and long-read next-generation sequencing. We established standardized workflows for strain typing and antimicrobial resistance profiling based on WGS. The results showed that short-read sequencing exhibited higher sensitivity for detecting nucleotide substitutions, while long-read sequencing had better concordance between genotypic and phenotypic resistance.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Van Ngo Thai Bich, Tan Khanh Nguyen, Thao Dao Thi Thu, Linh Thuy Thi Tran, Sa Vo Duong Nguyen, Ho Le Han, Long-Hung Dinh Pham, Trung Hoang Thanh, Van Hoa Duong, Tuan Anh Trieu, Manh Hung Tran, Phu Tran Vinh Pham
Summary: This study found that the ethyl acetate fraction extracted from Ampelopsis cantoniensis exhibited significant antibacterial activity against methicillin-resistant Staphylococcus aureus. Molecular docking and molecular dynamics methods revealed that the main compound, dihydromyricetin, could bind to the bacterial protein PBP2a at an allosteric site, thereby exerting its antibacterial effect. High performance liquid chromatography analysis confirmed dihydromyricetin as the major component of the ethyl acetate fraction. Therefore, natural products derived from Ampelopsis cantoniensis may serve as a potential therapy for methicillin-resistant Staphylococcus aureus.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Multidisciplinary Sciences
Bethel Kwansa-Bentum, Beatrice Awuradwoa Okine, Alberta D. Dayie, Patience B. Tetteh-Quarcoo, Fleischer C. N. Kotey, Eric S. Donkor, Nicholas T. K. D. Dayie
Summary: This study evaluated the antimicrobial effects of Eucalyptus grandis crude extracts against multidrug resistant bacteria. The results showed that except for the water extract, the other three extracts had antimicrobial activity, with the non-polar petroleum ether extract having the highest activity. Phytochemical screening also revealed the presence of bioactive compounds in the extracts. These findings suggest that Eucalyptus grandis could be potentially useful in treating infections caused by multidrug resistant bacteria.
Article
Chemistry, Medicinal
Baohua Xie, Zhinang Yin, Zhiye Hu, Junhui Lv, Chuanqian Du, Xiangping Deng, Yuan Huang, Qiuzi Li, Jian Huang, Kaiwei Liang, Hai-Bing Zhou, Chune Dong
Summary: This study reveals a novel series of potent ER alpha PROTACs based on the OBHSA scaffold, showing significant antiproliferation and ER alpha degradation activities against a broad spectrum of ER+ BC cells. Compound ZD12 exhibits excellent antitumor potency and ER alpha degradation activity, making it a promising drug candidate for endocrine-resistant BC treatment.
JOURNAL OF MEDICINAL CHEMISTRY
(2023)
Article
Biology
Yayu Xie, Pei Cao, Yuan Qin, Xiru Wu, Bingyao Huang, Kai Liu, Yonghong Liu
Summary: Kinases have been successfully targeted for drug discovery, but drug resistance is a concern. Assessing kinase mutations is essential for developing next-generation inhibitors. In this study, two mutants of ASK1 were constructed and their catalytic activity was investigated. Experimental results showed decreased binding affinity for the mutants compared to the wild type, and molecular dynamics simulations revealed the loss of hydrogen bond interaction as a possible cause for the decreased affinity.
COMPUTATIONAL BIOLOGY AND CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Ayodele O. Akinboye, Ibukun M. Famuyide, Inge-Marie Petzer, Lyndy J. McGaw
Summary: Bovine mastitis in dairy livestock production is a serious economic problem caused by Staphylococcus aureus and non-aureus staphylococcus. Antimicrobial resistance hampers control of mastitis. Nine South African plants were tested for their antibacterial and cytotoxic activities against mastitis-causing staphylococci. The extracts showed promising antibacterial effects and low cytotoxicity, particularly the acetone extracts. Further investigation is recommended.
APPLIED SCIENCES-BASEL
(2023)
