Journal
ARCHIVES OF PHARMACAL RESEARCH
Volume 31, Issue 6, Pages 727-735Publisher
PHARMACEUTICAL SOC KOREA
DOI: 10.1007/s12272-001-1219-0
Keywords
colon inflammation; Platycarya strobilacea; polyphenol; NF-kappa B; MCP-1; IL-8
Categories
Ask authors/readers for more resources
Oxidative stress and the activation of nuclear factor (NF)-kappa B play crucial roles in the pathogenesis of inflammatory bowel disease (IBD). In the present study, we examined the effects of the ethanol extract of Platycarya strobilacea Sieb. stem (EPS) on TNF-alpha-induced monocyte adhesion to HT29 human colon epithelial cells, an initial step of colon inflammation. EPS contained high amount of polyphenols (0.241 +/- 0.017 mg of catechin equivalent/g of extract) and showed substantial DPPH radical scavenging activity. In addition, EPS significantly suppressed TNF-alpha-induced reactive oxygen species (ROS) increase. Moreover, TNF-alpha-induced monocyte adhesion to HT29 colon epithelial cells was significantly suppressed by EPS in a concentration-dependent manner. The reduced adhesion by EPS was correlated with suppressed expression of MCP-1 and IL-8, the major chemokines in IBD. EPS also prevented the TNF-a-induced nuclear translocation of NF-kappa B, one of the redox-sensitive transcription factors, in a concentration-dependent manner. Taken together, our results suggest that the anti-oxidant components of EPS prevent TNF-alpha-induced NF-kappa B activation, chemokine induction, and monocyte adhesion at the site of intestinal inflammation.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available