Article
Endocrinology & Metabolism
Marc Leal-Julia, Jorge J. Vilches, Andrea Onieva, Sergi Verdes, Angela Sanchez, Miguel Chillon, Xavier Navarro, Assumpcio Bosch
Summary: This study characterized the proteome of neural tissues in a type 2 diabetes mouse model and identified differential protein expression profiles related to reduced glycolytic and TCA cycle metabolism, increased lipid catabolism, dysregulation of muscle-like proteins, altered cytoskeleton-related proteins, activation of inflammatory responses, and changes in glutathione metabolism and oxidative stress. These findings suggest that energy deficiency may play a key role in the neurodegeneration observed in diabetic neuropathy.
MOLECULAR METABOLISM
(2022)
Article
Biochemical Research Methods
Sarah A. Michaud, Helena Petrosova, Angela M. Jackson, Jamie C. McGuire, Nicholas J. Sinclair, Milan Ganguly, Ann M. Flenniken, Lauryl M. J. Nutter, Colin McKerlie, David Schibli, Derek Smith, Christoph H. Borchers
Summary: The study investigated the impact of different tissue processing methods on protein quantitation, finding that protein precipitation can enhance the abundance of target proteins. A simple yet effective tissue processing workflow was proposed for various mouse tissues, offering advantages such as simplicity and high sample quality.
JOURNAL OF PROTEOME RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Xiaojing Zhou, Abdullah Md. Sheikh, Ken-ichi Matsumoto, Shingo Mitaki, Abu Zaffar Shibly, Yuchi Zhang, A. Garu, Shozo Yano, Atsushi Nagai
Summary: Through proteomic analysis of urine exosomes in AD model mice, we identified proteins related to lipid metabolism and A beta metabolism in the early stages of AD, providing new insights into the underlying pathological mechanism of early AD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Oncology
Simon Sugar, Fanni Bugyi, Gabor Toth, Judit Papay, Ilona Kovalszky, Tamas Tornoczky, Laszlo Drahos, Lilla Turiak
Summary: Tackling and curing cancer, especially lung cancer, is a crucial challenge in biomedical research. By analyzing the proteomics profile of different lung cancer subtypes, researchers aim to identify dysregulated pathways and improve diagnostic panels for lung cancer. This study successfully identified differentially expressed proteins in lung cancer tissue and distinguished distinct molecular profiles for different lung cancer types.
Article
Chemistry, Analytical
Remilekun O. Lawal, Luke T. Richardson, Chao Dong, Fabrizio Donnarumma, Touradj Solouki, Kermit K. Murray
Summary: Deep-ultraviolet laser ablation with a pulsed 193 nm ArF excimer laser was used to extract intact proteins from tissue sections for proteomic analysis, demonstrating higher efficiency and yield compared to infrared laser ablation. The DUV laser ablation sampling resulted in approximately four times larger protein mass and number of identified proteins.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biochemistry & Molecular Biology
Xiuying Liang, Haijing Guan, Jingwen Sun, Yan Qi, Wenjuan Yao
Summary: This study identified novel proteins associated with Ang II infusion and showed that Ang II could regulate lipid metabolism, transportation, and adipocyte differentiation in PVAT. Key pathways involved fatty acid biosynthesis and various protein signaling cascades. These findings provide insights into the relationship between local RAS activation and PVAT dysfunction.
Article
Cell Biology
Xianglong Zhang, Liangbiao George Hu, Ying Lei, Marina Stolina, Oliver Homann, Songli Wang, Murielle M. Veniant, Yi-Hsiang Hsu
Summary: This study provides a transcriptomic and proteomic atlas of major organs in cynomolgus monkeys with obesity or T2D compared to healthy controls. Molecular changes mainly occur in adipose tissues of individuals with obesity, while extensive expression perturbations are observed in various tissues of T2D individuals. Immune-response-related pathways are upregulated in obesity and T2D, while metabolism and mitochondrial pathways are downregulated.
Article
Pathology
Pouya Jamshidi, Jonathan Levi, Maria Jose Suarez, Roxana Rivera, Nicholas Mahoney, Charles G. Eberhart, Avi Rosenberg, Fausto J. Rodriguez
Summary: Ocular amyloidomas involving the ocular adnexa exhibit a variety of amyloid-related and immunoglobulin-associated peptides. The lambda light chain is predominantly present, but mixed patterns and rarely kappa light chain restriction may also be observed. The study highlights the clinicopathologic and proteomic characteristics of periocular amyloid deposition in a retrospective review of cases from 1991 to 2020.
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Jacky M. K. Kwong, Joseph Caprioli, Joanne C. Y. Lee, Yifan Song, Feng-Juan Yu, Jingfang Bian, Ying-Hon Sze, King-Kit Li, Chi-Wai Do, Chi-Ho To, Thomas Chuen Lam
Summary: This study investigated the proteomic changes in the retina after optic nerve transection in rats. Using high-resolution mass spectrometry, they identified several differentially expressed proteins related to retinal ganglion cell damage, such as ALDH1A1 and SNCG.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Chemistry, Analytical
Mingxia Liu, Daiyu Miao, Shaojie Qin, Huwei Liu, Yu Bai
Summary: This review summarizes the comprehensive and essential developments of mass spectrometric immunoassay (MSIA) and its bioanalytical applications. MSIA overcomes the limitation of low ionization efficiency of biomacromolecules through mass tag-based detection, enabling highly sensitive detection and showing great potential in bioanalysis.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Simeon C. Daeschler, Rebecca Wienbruch, Catalina Bursacovschi, Kim Sophie Zimmermann, Selam Bekure Nemariam, Leila Harhaus, Ulrich Kneser, Alfons Dehe, Achim Bittner
Summary: This study reviewed previous approaches to direct nerve compression pressure measurement (DNCPM) and evaluated the feasibility and reliability of currently available microsensor systems in preclinical nerve compression models. The results showed that measurement variability caused by unreliable sensor positioning is a key limitation of presently available sensors. The authors suggest redesigning systems for reliable measurements.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Hannah Voss, Manuela Moritz, Penelope Pelczar, Nicola Gagliani, Samuel Huber, Vivien Nippert, Hartmut Schlueter, Jan Hahn
Summary: This study investigates the applicability of infrared laser tissue sampling for proteome analysis of different cell layers of murine intestine. By sampling directly from intact fresh-frozen tissue, the study successfully obtained the different proteomes of colon tissue's different cell layers. This new sampling method provides higher protein yield and identification compared to conventional mechanical homogenization.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ruta Navakauskiene, Sandra Baronaite, Dalius Matuzevicius, Natalija Krasovskaja, Grazina Treigyte, Audrone Arlauskiene, Dalius Navakauskas
Summary: Mass spectrometry-based proteomics was used to analyze amniotic fluid samples and identify proteins related to normal and polyhydramnios pregnancies. Quantitative changes in proteins were evaluated through computational analysis and comparison of protein maps. The study found that certain proteins showed increased levels in pathological pregnancies, suggesting their association with immune response, inflammation, and abnormal development. This research provides insights into the mechanisms underlying pathological pregnancies and fetal abnormalities.
Article
Clinical Neurology
Roshan Dhanapalaratnam, Tushar Issar, Ann M. Poynten, Kerry-Lee Milner, Natalie C. G. Kwai, Arun Krishnan
Summary: This study found that peripheral nerve ultrasound can serve as a marker for the presence and severity of peripheral neuropathy in type 2 diabetes, with high sensitivity and specificity. The tibial nerve CSA correlates with neuropathy severity.
EUROPEAN JOURNAL OF NEUROLOGY
(2022)
Article
Biology
Iris Wagner, Linda Grigoraki, Peter Enevoldson, Michael Clarkson, Sam Jones, Jane L. L. Hurst, Robert J. J. Beynon, Hilary Ranson
Summary: The rapid evaporative ionisation mass spectrometry (REIMS) method can accurately identify the species and age of mosquitoes, with high accuracy in both laboratory-reared and wild populations.
Article
Clinical Neurology
Sean W. Taylor, Ruple S. Laughlin, Neeraj Kumar, Brent Goodman, Christopher J. Klein, Peter J. Dyck, P. James B. Dyck
JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY
(2017)
Article
Medicine, General & Internal
Christopher J. Klein, Tatiana M. Foroud
MAYO CLINIC PROCEEDINGS
(2017)
Article
Medicine, General & Internal
Eelco F. M. Wijdicks, Christopher J. Klein
MAYO CLINIC PROCEEDINGS
(2017)
Article
Clinical Neurology
Wei Wang, William J. Litchy, Michelle L. Mauermann, P. James B. Dyck, Angela Dispenzieri, Jay Mandrekar, Peter J. Dyck, Christopher J. Klein
Article
Clinical Neurology
Rajat Lahoria, Sean J. Pittock, Avi Gadoth, Janean K. Engelstad, Vanda A. Lennon, Christopher J. Klein
Article
Clinical Neurology
Mohamed Kazamel, Christopher J. Klein, Eduardo E. Benarroch, Mrinal M. Patnaik, Jennifer A. Tracy
Article
Clinical Neurology
Peter J. Dyck, John C. Kincaid, P. James B. Dyck, Vinay Chaudhry, Namita A. Goyal, Christina Alves, Hayet Salhi, Janice F. Wiesman, Celine Labeyrie, Jessica Robinson-Papp, Marcio Cardoso, Matilde Laura, Katherine Ruzhansky, Andrea Cortese, Thomas H. Brannagan, Julie Khoury, Sami Khella, Marcia Waddington-Cruz, Joao Ferreira, Annabel K. Wang, Marcus V. Pinto, Samar S. Ayache, Merrill D. Benson, John L. Berk, Teresa Coelho, Michael Polydefkis, Peter Gorevic, David H. Adams, Violaine Plante-Bordeneuve, Carol Whelan, Giampaolo Merlini, Stephen Heitner, Brian M. Drachman, Isabel Conceicao, Christopher J. Klein, Morie A. Gertz, Elizabeth J. Ackermann, Steven. G. Hughes, Michelle. L. Mauermann, Rito Bergemann, Karen A. Lodermeier, Jenny L. Davies, Rickey E. Carter, William J. Litchy
Article
Clinical Neurology
Divyanshu Dubey, Vanda A. Lennon, Avi Gadoth, Sean J. Pittock, Eoin P. Flanagan, John E. Schmeling, Andrew McKeon, Christopher J. Klein
Editorial Material
Clinical Neurology
Komal Rastogi, Christopher J. Klein, John E. O'Toole, Miral D. Jhaveri, Rabia Malik
Editorial Material
Clinical Neurology
Amy C. Visser, Christopher J. Klein
Article
Clinical Neurology
E. Matthew Hoffman, James C. Watson, Jennifer St Sauver, Nathan P. Staff, Christopher J. Klein
Article
Clinical Neurology
Divyanshu Dubey, Sean J. Pittock, Cecilia R. Kelly, Andrew McKeon, Alfonso Sebastian Lopez-Chiriboga, Vanda A. Lennon, Avi Gadoth, Carin Y. Smith, Sandra C. Bryant, Christopher J. Klein, Allen J. Aksamit, Michel Toledano, Bradley F. Boeve, Jan-Mendelt Tillema, Eoin P. Flanagan
ANNALS OF NEUROLOGY
(2018)
Article
Clinical Neurology
Jennifer M. Martinez-Thompson, Melissa R. Snyder, Michael Ettore, Andrew Mckeon, Sean J. Pittock, Matthew M. Roforth, Jay Mandrekar, Michelle L. Mauermann, Bruce V. Taylor, James B. Dyck, Anthony J. Windebank, Christopher J. Klein
Article
Clinical Neurology
Avi Gadoth, Anastasia Zekeridou, Christopher J. Klein, Colton J. Thoreson, Masoud Majed, Divyanshu Dubey, Eoin P. Flanagan, Andrew McKeon, Sarah M. Jenkins, Vanda A. Lennon, Sean J. Pittock
ANNALS OF CLINICAL AND TRANSLATIONAL NEUROLOGY
(2018)
Article
Clinical Neurology
Christopher J. Klein, Yanhong Wu, Mark E. Jentoft, Georges Mer, Robert J. Spinner, P. James B. Dyck, Peter J. Dyck, Michelle L. Mauermann
ANNALS OF NEUROLOGY
(2017)