Review
Biochemistry & Molecular Biology
Sylwia Smolinska, Dario Antolin-Amerigo, Florin-Dan Popescu, Marek Jutel
Summary: TSLP is a critical cytokine in allergy and asthma development, primarily produced by epithelial cells. It functions as a potent immune system activator through interaction with its receptor complex. Understanding the interplay between TSLP and dysfunctional epithelium provides insights into allergy and asthma pathogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Immunology
Si-Hang Wang, Ya-Gang Zuo
Summary: TSLP, particularly the long form, plays a crucial role in immune-mediated skin diseases, driving Th2 and Th17 immune responses, inducing itch, and being associated with skin fibrosis and epidermal hyperplasia. Targeting TSLP may have significant implications for the treatment of these diseases in the future.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Na-Ra Han, Seong-Gyu Ko, Phil-Dong Moon, Hi-Joon Park
Summary: Chloroquine (CQ) exhibits anti-inflammatory effects by modulating TSLP levels in mast cells and reducing ear edema thickness, as well as TSLP levels in ear tissue and serum. The down-regulation of CASP1, NF-kappa B, and other signaling cascades by CQ contributes to its protective role against TSLP-mediated inflammatory disorders.
BIOMEDICINE & PHARMACOTHERAPY
(2021)
Article
Immunology
Dina Leth Moller, Katrine Kielsen, Claus Henrik Nielsen, Henrik Sengelov, Anders Elm Pedersen, Lars Peter Ryder, Klaus Muller
Summary: This study suggests a role of TSLP in immune reconstitution and alloreactivity post-HSCT.
IMMUNOPHARMACOLOGY AND IMMUNOTOXICOLOGY
(2022)
Article
Respiratory System
Changhui Yu, Wufeng Huang, Zicong Zhou, Shixiu Liang, Zili Zhou, Jieyi Liu, Haijing Zhao, Laiyu Liu, Hangming Dong, Fei Zou, Shaoxi Cai
Summary: The short isoform of TSLP (sTSLP) inhibits airway inflammation in asthma by occupying the binding site of the long isoform of TSLP (lTSLP) in the TSLPR and IL-7R receptor complex, thereby reducing aerobic glycolysis and inflammatory cytokine production.
RESPIRATORY RESEARCH
(2022)
Article
Oncology
Ranya Guennoun, Jennet Hojanazarova, Kathryn E. Trerice, Marjan Azin, Matthew T. McGoldrick, Erik B. Schiferle, Michael P. Stover, Shadmehr Demehri
Summary: This study demonstrates the potential of immunotherapy for lung cancer by examining the impact of Thymic Stromal Lymphopoietin (TSLP) cytokine induction on early lung cancer development. TSLP induction suppresses the development of invasive lung tumors, dependent on CD4(+) T cells. This highlights the importance of adaptive immune response in protecting against lung cancer progression.
Article
Biotechnology & Applied Microbiology
Sara A. Watson, Yousef Javanmardi, Luca Zanieri, Somayeh Shahreza, Roberta Ragazzini, Paola Bonfanti, Emad Moeendarbary
Summary: The human thymus is crucial for T-cell maturation, and understanding the recruitment of hematopoietic stem cells (HSCs) to the thymus is important. In this study, a 3D microfluidic assay was developed to model HSC extravasation across the endothelium, and it was found that thymic stromal cells (TSCs) play a critical role in increasing the extravasation rates of HSCs. Complex gene expressions between endothelial cells, thymic epithelial cells (TECs), and interstitial cells (TICs) were also found to modulate HSC extravasation.
BIOENGINEERING & TRANSLATIONAL MEDICINE
(2023)
Review
Allergy
Lorenzo Salvati, Laura Maggi, Francesco Annunziato, Lorenzo Cosmi
Summary: Epithelial cytokines, particularly TSLP, play a crucial role in asthma outcomes, and the anti-TSLP monoclonal antibody tezepelumab has proven to be an effective alternative treatment. Biomarker identification will help predict responders and personalize asthma treatment.
CURRENT OPINION IN ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Wanlin Li, Chenghui Liao, Jing Du, Jing Hu, Lu Wang, Xun Song, Zhendan He, Xiaohua Xiao, Liang Ye
Summary: Two isoforms of TSLP, lTSLP and sTSLP, exist in rheumatoid arthritis (RA), with lTSLP being predominant in active RA and sTSLP in inactive RA and healthy controls. lTSLP expression is associated with disease activity and proinflammatory cytokine expression. lTSLP promotes the expression of proinflammatory cytokines in PBMCs of RA, while inhibiting the expression of anti-inflammatory cytokines.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Cardiac & Cardiovascular Systems
Yuhao Zhao, Yeping Zhang, Zongsheng Guo, Zheng Ma, Ye Liu, Chunming Han, Xinchun Yang, Lei Zhao
Summary: Plasma TSLP levels were higher in AMI patients compared to UA patients, and lower TSLP levels were associated with MACE after AMI. Follow-up analysis showed that TSLP was a negative predictor of MACE in AMI patients.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2022)
Article
Allergy
Adrien Brulefert, Astrid Hoste, Quentin Muller, Jean-Daniel Fauny, Christopher G. Mueller, Vincent Flacher
Summary: Our study found that vitamin D3 can induce skin dendritic cells to differentiate into Th2 cells producing IL-4 and IL-13, while also triggering the release of TSLP. Blocking the TSLP receptor in some donors eliminated IL-13 production but had no effect on IL-4+ Th2 cells. Vitamin D3-elicited CD14+ dendritic cells were able to promote IL-4+ Th2 cells in a TSLP-independent manner.
Article
Medicine, General & Internal
Simone Marcella, Angelica Petraroli, Luisa Cane, Anne Lise Ferrara, Remo Poto, Roberta Parente, Francesco Palestra, Leonardo Cristinziano, Luca Modestino, Maria Rosaria Galdiero, Maria Monti, Gianni Marone, Massimo Triggiani, Gilda Varricchi, Stefania Loffredo
Summary: Mastocytosis is a heterogeneous disease characterized by uncontrolled proliferation and increased density of mast cells. In this study, it was found that serum concentrations of Thymic stromal lymphopoietin (TSLP) were lower in patients with mastocytosis compared to healthy donors. TSLP was found to be a substrate for human mast cell tryptase, indicating a novel connection between these mediators in mastocytosis.
EUROPEAN JOURNAL OF INTERNAL MEDICINE
(2023)
Review
Immunology
Luhong Cao, Weiwei Qian, Wanlin Li, Zhiyue Ma, Shenglong Xie
Summary: Previously believed to have similar functions as type I interferon, type III interferon (IFN-III) is now shown to play a unique role in innate antiviral immune responses. The regulatory activity of IFN-III in adaptive immune responses has not been clearly reported yet due to receptor expression limitations. However, this study reviewed the adjuvant, antiviral, antitumor, and disease-moderating activities of IFN-III in adaptive immunity, and elucidated the mechanisms of IFN-III in mediating the adaptive antiviral immune response through the TSLP axis.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Chieh-Hsin Wu, Chun-Ching Lu, Chao-Lan Huang, Ming-Kung Wu, Ying-Yi Lu
Summary: The study demonstrated that TSLP plays a role in nerve after injury and may serve as a potential therapeutic target for pain regulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Respiratory System
Bakari Ibrahim, Djamal Achour, Farid Zerimech, Patricia de Nadai, Valerie Siroux, Anne Tsicopoulos, Regis Matran, Vanessa Granger, Rachel Nadif
Summary: In non-severe asthma, TSLP may be an interesting cytokine and its determinants of circulating levels could be considered in asthma management.
Letter
Respiratory System
Sophie M. Poznanski, Ana Portillo, Manali Mukherjee, Anurag Bhalla, Kiho Son, Ali A. Ashkar, Nader Khalidi, Parameswaran Nair
EUROPEAN RESPIRATORY JOURNAL
(2022)
Review
Pharmacology & Pharmacy
Brittany Salter, Paige Lacy, Manali Mukherjee
Summary: Recent advancements in therapeutic strategies for airway inflammation in asthma are providing alternatives to corticosteroid treatment. Targeting eosinophilic inflammation with novel biologics has shown significant success in managing asthma symptoms. Monoclonal antibody treatments have improved clinical outcomes for asthma patients. However, a significant challenge remains in identifying patients who do not respond adequately to targeted treatments. Understanding the underlying immunology and determining the immune makeup of individual patients prior to prescribing monoclonal antibody therapy is crucial for precision medicine in asthma treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Respiratory System
Ditte K. Klein, Alexander Silberbrandt, Laurits Frossing, Morten Hvidtfeldt, Anna von Buelow, Parameswaran Nair, Manali Mukherjee, Celeste Porsbjerg
Summary: In patients with severe asthma, a history of smoking is associated with eosinophilic airway inflammation and activation, as well as relative insensitivity to steroids and airway autoimmunity.
EUROPEAN RESPIRATORY JOURNAL
(2022)
Article
Immunology
Sizun Jiang, Chi Ngai Chan, Xavier Rovira-Clave, Han Chen, Yunhao Bai, Bokai Zhu, Erin McCaffrey, Noah F. Greenwald, Candace Liu, Graham L. Barlow, Jason L. Weirather, John Paul Oliveria, Tsuguhisa Nakayama, Ivan T. Lee, Matthias S. Matter, Anne E. Carlisle, Darci Philips, Gustavo Vazquez, Nilanjan Mukherjee, Kathleen Busman-Sahay, Michael Nekorchuk, Margaret Terry, Skyler Younger, Marc Bosse, Janos Demeter, Scott J. Rodig, Alexandar Tzankov, Yury Goltsev, David Robert McIlwain, Michael Angelo, Jacob D. Estes, Garry P. Nolan
Summary: Understanding the mechanisms of HIV tissue persistence requires the ability to visualize tissue microenvironments where infected cells reside. However, technological limitations hinder our ability to dissect the cellular components of HIV reservoirs. In this study, a protein and nucleic acid in situ imaging (PANINI) technique was developed to simultaneously quantify DNA, RNA, and protein levels within tissue compartments. By combining PANINI with multiplexed ion beam imaging (MIBI), over 30 parameters could be measured simultaneously. This technology enables the spatial dissection of cellular phenotypes, functional markers, and viral events resulting from infection, providing a framework for studying tissue responses in other infectious diseases and tumor biology.
Article
Cell Biology
Marla C. Glass, David R. Glass, John-Paul Oliveria, Berenice Mbiribindi, Carlos O. Esquivel, Sheri M. Krams, Sean C. Bendall, Olivia M. Martinez
Summary: Regulatory B cells (Bregs) secrete interleukin-10 (IL-10) to suppress immune responses, but there is no definitive immunophenotype to identify these B cells. Multiple functional subsets of B cells can produce IL-10, and there is no unique phenotype for IL-10(+) B cells. Furthermore, a significant portion of IL-10(+) B cells also express the pro-inflammatory cytokines IL-6 and tumor necrosis factor alpha (TNF-alpha). However, operationally tolerant liver transplant recipients have an enrichment of IL-10(+) but not TNF-alpha(+) or IL-6(+) B cells compared to those receiving immunosuppression. Therefore, IL-10-producing B cells in humans constitute an induced and transient state arising from various subsets of B cells, which may contribute to the maintenance of immune homeostasis.
Article
Critical Care Medicine
Kiho Son, Kate Miyasaki, Brittany Salter, Dessi Loukov, Joseph Chon, Nan Zhao, Katherine Radford, Chynna Huang, Nicola LaVigne, Anna Dvorkin-Gheva, Paige Lacy, Terence Ho, Dawn M. E. Bowdish, Parameswaran Nair, Manali Mukherjee
Summary: Autoantibodies against macrophage scavenger receptors in eosinophilic asthma airways may impede effective host defenses and lead to recurrent infective bronchitis.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2023)
Letter
Allergy
Hana Serajeddini, Carmen Venegas Garrido, Anurag Bhalla, Melanie Kjarsgaard, Chynna Huang, Nicola LaVigne, Katherine Radford, Kayla Zhang, Terence Ho, Sarah Svenningsen, Manali Mukherjee, Parameswaran Nair
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY-IN PRACTICE
(2023)
Article
Multidisciplinary Sciences
M. Monsur Ali, Michael G. G. Wolfe, Manali Mukherjee, Katherine Radford, Zil Patel, Dawn White, Julijana Milojevic, Alfredo Capretta, Parameswaran Nair, John D. D. Brennan
Summary: In this study, a simple and efficient method for detecting the activity level of eosinophils in the airways to guide anti-inflammatory therapy for airway diseases was developed through in vitro selection and optimization of DNA aptamers.
SCIENTIFIC REPORTS
(2022)
Editorial Material
Respiratory System
Manali Mukherjee, Martin Kolb
EUROPEAN RESPIRATORY JOURNAL
(2023)
Letter
Respiratory System
Imran Satia, Alexandra J. Mayhew, Nazmul Sohel, Om Kurmi, Kieran J. Killian, Megan E. O'Connell, Paul M. O'Byrne, Parminder Raina
Letter
Biochemistry & Molecular Biology
Nadia Suray Tan, Manali Mukherjee, Sheau Yng Lim, Angeline Rouers, You Yi Hwang, Chung Hwee Thiam, W. S. Daniel Tan, Wupeng Liao, W. S. Fred Wong, Mei Fong Liew, Parameswaran Nair, Anis Larbi, De Yun Wang, Katja Fink, Veronique Angeli, Hui Fang Lim
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Respiratory System
Imran Satia, Alexandra J. Mayhew, Nazmul Sohel, Om Kurmi, Kieran J. Killian, Paul M. O'Byrne, Parminder Raina
Summary: This study reveals a bidirectional relationship between chronic cough and depressive symptoms and psychological distress, and personality traits do not independently influence the development of chronic cough.
Article
Biochemical Research Methods
Nora Vivanco Gonzalez, John-Paul Oliveria, Dmitry Tebaykin, Geoffrey T. Ivison, Kaori Mukai, Mindy M. Tsai, Luciene Borges, Kari C. Nadeau, Stephen J. Galli, Albert G. Tsai, Sean C. Bendall
Summary: This study utilized mass cytometry to capture distinct phenotypes of granulocytes, and through optimized protocols, achieved in-depth characterization of basophils, eosinophils, and neutrophils. This research is of great significance for understanding the roles of granulocytes in homeostasis and immune activation.
Article
Respiratory System
Terence Ho, Matthew Nichols, Gayatri Nair, Katherine Radford, Melanie Kjarsgaard, Chynna Huang, Anurag Bhalla, Nicola Lavigne, Manali Mukherjee, Michael Surette, Joseph Macri, Parameswaran Nair
Summary: Excessive iron in pulmonary macrophages may contribute to recurrent airway infections in COPD, potentially leading to increased frequency of infective exacerbations by affecting immune cell function.
RESPIRATORY RESEARCH
(2022)
Article
Respiratory System
Anurag Bhalla, Melanie Kjarsgaard, Nicola LaVigne, Katherine Radford, Manali Mukherjee, Parameswaran Nair
Summary: The study aimed to characterize patients with increased sputum bronchial epithelial cells (BECs) based on clinical and inflammatory variables. The results showed that elevated BECs were associated with younger age, higher corticosteroid dose, symptoms of cough and wheezing, and predominantly an eosinophilic or paucigranulocytic inflammatory endotype.
CANADIAN JOURNAL OF RESPIRATORY CRITICAL CARE AND SLEEP MEDICINE
(2022)