Review
Polymer Science
Aiah A. El-Rashidy, Sara El Moshy, Israa Ahmed Radwan, Dina Rady, Marwa M. S. Abbass, Christof E. Doerfer, Karim M. Fawzy El-Sayed
Summary: The stiffness of the extracellular matrix plays a regulatory role in directing the osteogenic differentiation of MSCs, with MSCs sensing and responding to their ECM to undergo differentiation. Customizing scaffold materials to mimic cells' natural environment is crucial in the tissue engineering process.
Article
Engineering, Biomedical
Ho-Ying Wan, Rita Lih Ying Shin, Jack Chun Hin Chen, Marisa Assuncao, Dan Wang, Susie K. Nilsson, Rocky S. Tuan, Anna Blocki
Summary: The study demonstrated that supplementation of Dextran sulfate (DxS) in osteogenically induced cultures of human mesenchymal stem cells (MSCs) amplified the deposition of osteoblastic extracellular matrix (ECM). This enhancement led to increased collagen type I deposition and osteogenic gene expression, as well as enrichment in bone morphogenetic protein 2 (BMP-2), suggesting a potential role of DxS in promoting osteogenic differentiation by MSCs.
ACTA BIOMATERIALIA
(2022)
Article
Cell & Tissue Engineering
Arseniy Lobov, Polina Kuchur, Aigul Khizhina, Anastasia Kotova, Andrei Ivashkin, Daria Kostina, Polina Klausen, Evgeniia Khokhlova, Egor Repkin, Kseniia Postnikova, Daria Perepletchikova, Evgeny Denisov, Tatiana Gerashchenko, Rashid Tikhilov, Svetlana Bozhkova, Andrey Sereda, Vitaliy Karelkin, Natella Enukashvily, Anna Malashicheva
Summary: In this study, we conducted a comparative analysis of mesenchymal cell primary cultures from various sources and found differences in their ability to undergo osteogenic differentiation and produce extracellular matrix. Based on the analysis of secretome, proteome, and transcriptome, we classified them into dental stem cells, mesenchymal stem cells, and fetal stem cells. Although they have differences, mesenchymal cells from all sources secreted core bone extracellular matrix-associated proteins. These findings shed light on the distinctive characteristics of mesenchymal stem cells and provide insights into their potential applications in regenerative medicine and the inherent diversity of mesenchymal cells in vivo.
Review
Biochemistry & Molecular Biology
Nicolas Thomas Iannozzi, Valentina Marchica, Denise Toscani, Jessica Burroughs Garcia, Nicola Giuliani, Paola Storti
Summary: Multiple myeloma is characterized by unregulated proliferations of plasma cells in bone marrow, which is influenced by the bone marrow microenvironment. The alterations of mesenchymal stromal cells and osteoblasts in multiple myeloma patients contribute to myeloma-associated bone disease. Understanding the characteristics and molecular pathways of these cells can provide insights into the pathophysiology of multiple myeloma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Shuzuo Zhou, Gang Zhang, Kun Wang, Zhong Yang, Yinghui Tan
Summary: The expression of miR-141-3p was found to decrease during the osteogenic differentiation of human BMSCs. The mRNA expression of ALP, RUNX2, and OCN, as well as the relative ALP activities, were reduced in the miR-141-3p mimic group and increased in the inhibitor group. SIRT1 was identified as a target of miR-141-3p, and its expression was decreased in the miR-141-3p mimic group. SIRT1 reversed the inhibitory effect of miR-141-3p on the osteogenic differentiation ability of BMSCs.
STEM CELLS INTERNATIONAL
(2023)
Article
Biochemistry & Molecular Biology
Aleksandra Kostina, Arseniy Lobov, Daria Semenova, Artem Kiselev, Polina Klausen, Anna Malashicheva
Summary: Despite significant progress in bone tissue regeneration, the early mechanisms of osteogenic differentiation remain poorly understood. Various cells can undergo osteogenic transformation, with Notch signaling playing a key role in this process. Research demonstrates differences in the ability of different cell types to undergo Notch-dependent osteogenic differentiation.
Article
Biochemistry & Molecular Biology
Paulina Kazimierczak, Malgorzata Koziol, Agata Przekora
Summary: The newly developed biomaterial showed high biocompatibility and influenced macrophage polarization and osteoblast differentiation, playing an immunomodulatory role. The study demonstrated a low risk of inflammatory response and induced macrophage polarization into the osteogenic phenotype M2, making it a promising bone scaffold for regenerative medicine applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Plant Sciences
Avinash Sanap, Kalpana Joshi, Tejas Shah, Girish Tillu, Ramesh Bhonde
Summary: The study showed that Piper longum L. fruit extract significantly increased proliferation of WJMSCs and enhanced Ca2+ accumulation and matrix mineralization, along with promoting alkaline phosphatase (ALP) activity and the expression of key gene mRNAs. This suggests that the fruit extract can enhance osteogenic differentiation of WJMSCs, potentially serving as an anti-osteoporotic agent.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
A. Jin, H. Xu, X. Gao, S. Sun, Y. Yang, X. Huang, X. Wang, Y. Liu, Y. Zhu, Q. Dai, Q. Bian, L. Jiang
Summary: This study constructed a single-cell atlas of mouse alveolar bone cells and identified a group of cells with high expression of Fat4 (Fat4(+) cells). These cells may initiate a distinct osteogenic differentiation trajectory in the alveolar bone.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Engineering, Biomedical
Jana Dvorakova, Lucie Wiesnerova, Petra Chocholata, Vlastimil Kulda, Lukas Landsmann, Miroslava Cedikova, Michaela Kripnerova, Lada Eberlova, Vaclav Babuska
Summary: Bone regeneration is a challenging process in medicine, and various materials are being tested for replacing missing bone or teeth. Different cell types have their own advantages and limitations in the proliferation and differentiation of bone tissue. This review provides an overview of cell models, such as osteoblasts, mesenchymal stem cells, and osteosarcoma cells, for evaluating the biocompatibility of materials used in bone tissue research.
BIOMEDICAL ENGINEERING ONLINE
(2023)
Article
Cell & Tissue Engineering
Changzhen Wang, Hongmei Ning, Jiao Gao, Teng Xue, Ming Zhao, Xiaoxia Jiang, Xiaoming Zhu, Ximin Guo, Hong Li, Xiaoyan Wang
Summary: This study investigated the impact of hematopoietic cells on MSCs and found that partial deletion of the Pten gene in hematopoietic cells can lead to abnormal differentiation of MSCs. There is a reciprocal relationship between hematopoietic cells and MSCs, with an important role in bone development.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Medicine, Research & Experimental
Lia Pulsatelli, Cristina Manferdini, Elena Gabusi, Erminia Mariani, Francesco Ursini, Jacopo Ciaffi, Riccardo Meliconi, Gina Lisignoli
Summary: This study describes the clinical features of a PPRD patient and the biological characterization of PPRD mesenchymal stromal cells (MSCs) and osteoblasts (OBs). The findings suggest that MSCs may play a crucial role in the pathological mechanisms of PPRD, and compromised functionality of MSCs may lead to an imbalance in bone homeostasis.
EUROPEAN JOURNAL OF MEDICAL RESEARCH
(2022)
Review
Pharmacology & Pharmacy
Jinli Zhang, Zhihe Liu, Yang Luo, Xiaojian Li, Guowei Huang, Huan Chen, Aiguo Li, Shengnan Qin
Summary: Flavonoids have the potential to regulate the osteogenic differentiation of mesenchymal stem cells (MSCs), making them a promising therapeutic application in bone disorders and bone tissue engineered constructs.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Cell Biology
Juan C. Henao, Adriana Grismaldo, Alfonso Barreto, Viviana M. Rodriguez-Pardo, Claudia Camila Mejia-Cruz, Efrain Leal-Garcia, Rafael Perez-Nunez, Patricio Rojas, Ramon Latorre, Ingrid Carvacho, Yolima P. Torres
Summary: The study demonstrates that TRPM8 channels are expressed in human bone marrow MSCs and are involved in osteogenic differentiation, with agonists promoting osteogenic differentiation and antagonists leading to a decrease in osteogenic differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chiara Greggi, Ida Cariati, Federica Onorato, Riccardo Iundusi, Manuel Scimeca, Umberto Tarantino
Summary: The study revealed that PTX3 plays a role in osteogenic differentiation and possesses osteoinductive capacity, making it a potential biomarker and therapeutic agent for osteoporosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
