Article
Biochemistry & Molecular Biology
Fen Li, Lianyun Lin, Hao Wang, Wenbo Duan, Linlin Yuan, Peng Cao, Zhiguang Yuchi, Shaoying Wu
Summary: Our study shows that while the mutations (LF)-F-1002 and (LF)-F-1002/D(941)G have significant impacts on the response to pyrethroids, the mutation D(941)G alone does not have a major effect compared to the wild type channel. This suggests that some mutations may not lead to resistance on their own but can enhance resistance when combined with other mutations.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Entomology
Spyridon Vlogiannitis, Wim Jonckheere, Dries Laget, Dirk C. de Graaf, John Vontas, Thomas Van Leeuwen
Summary: The Varroa destructor mite, considered a major threat to beekeeping, has developed resistance to synthetic acaricides like fluvalinate and flumethrin. A study in Flanders, Belgium found that a common L925V substitution in the mites is associated with resistance to flumethrin, leading to a significant increase in resistance levels. This highlights the importance of careful consideration when using pyrethroid-based varroacides in Flanders.
EXPERIMENTAL AND APPLIED ACAROLOGY
(2021)
Article
Entomology
Haung Naw, Tuan Cuong Vo, Huong Giang Le, Jung-Mi Kang, Yi Yi Mya, Moe Kyaw Myint, Tong-Soo Kim, Ho-Joon Shin, Byoung-Kuk Na
Summary: This study identified high levels of kdr mutations in the Ae. aegypti population in the Mandalay area of Myanmar, suggesting widespread resistance to pyrethroid insecticides. The results underscore the need for an effective vector control program in Myanmar to address the increasing resistance in mosquitoes.
Article
Agronomy
Kentaro Itokawa, Shogo Furutani, Aki Takaoka, Yoshihide Maekawa, Kyoko Sawabe, Osamu Komagata, Takashi Tomita, Jose Luiz de Lima Filho, Luiz Carlos Alves, Shinji Kasai
Summary: Aedes aegypti is a highly effective mosquito vector for diseases like dengue fever, yellow fever, and Zika. Resistance to pyrethroids, the main insecticides, is spreading globally within this species. This study identified new amino acid substitutions in the VGSC gene, potentially contributing to pyrethroid resistance in Ae. aegypti, and showed that certain haplotypes have equal or slightly stronger resistance to different types of pyrethroids. Further research is needed to understand the role of these substitutions in pyrethroid susceptibility.
PEST MANAGEMENT SCIENCE
(2021)
Article
Entomology
Boris S. Zhorov, Ke Dong
Summary: This study used the AlphaFold2 neural network to generate new models of mosquito and cockroach sodium channels, and used computational methods to investigate the interactions between pyrethroids and sodium channels. The results suggest that mutations located beyond the receptor sites can alter the action of pyrethroids and confer resistance by affecting the geometry of the receptor sites. This study provides valuable information for the design of novel pyrethroids based on their structure.
Article
Agronomy
Claudia Tschesche, Michael Bekaert, David Bassett, Chris Mitchell, Ben North, Sally Boyd, Greta Carmona-Antonanzas, James E. Bron, Armin Sturm
Summary: The study found that deltamethrin resistance in Lepeophtheirus salmonis is unrelated to the LsNa(v)1.3 I936V mutation, but is closely associated with mtDNA mutations.
PEST MANAGEMENT SCIENCE
(2021)
Article
Agriculture, Multidisciplinary
Kaiyang Liu, Shuyue Ma, Kun Zhang, Ruibo Gao, Peng Cao, Haifeng Jin, Zhiguang Yuchi, Shaoying Wu
Summary: The L1014S mutation in the voltage-gated sodium channel gene reduces the pyrethroid sensitivity and affects the channel gating in Xenopus oocytes. This study provides insights into the mechanism of pyrethroid resistance in Blattella germanica and predicts the residues associated with the risk of resistance.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Veterinary Sciences
M. Benito-Murcia, R. Martin-Hernandez, A. Meana, C. Botias, M. Higes
Summary: This study investigated the presence of pyrethroid resistance mutations in Varroa mites collected from apiaries across Spain. The study identified the combination of M918L and L925V mutations in the gene encoding the voltage-gated sodium channel (VGSC), which produces a higher level of resistance compared to the L925V mutation alone.
RESEARCH IN VETERINARY SCIENCE
(2022)
Article
Agronomy
Jamie C. Freeman, Ker San Miguel, Jeffrey G. Scott
Summary: The study found that the super-kdr allele incurs a significant fitness cost compared to other alleles, with its frequency decreasing significantly after 25 generations. In contrast, the fitness of the kdr, kdr-his, and susceptible alleles were similar. The most significant change in genotype frequency was observed for the super-kdr/super-kdr genotype, which was no longer detected after 25 generations.
PEST MANAGEMENT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Huiming Wu, Jiali Qian, Zhanyi Xu, Ru Yan, Guonian Zhu, Shaoying Wu, Mengli Chen
Summary: Aedes aegypti is responsible for transmitting diseases such as dengue and chikungunya. The L982W mutation in the pyrethroid receptor site 1 reduces sensitivity to pyrethroids and DDT. Understanding the molecular basis of this mutation is important for monitoring pest resistance to insecticides.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Entomology
Ruibo Gao, Rongcai Lu, Xinyao Qiu, Likui Wang, Kun Zhang, Shaoying Wu
Summary: This study found that Megalurothrips usitatus, the main pest of cowpea in China, exhibits resistance to pyrethroids and a mutation in the I873S gene may be related to this resistance. These findings will contribute to the research on pyrethroid resistance in M. usitatus and provide guidance for improving its management system in the future.
Article
Biochemistry & Molecular Biology
Ruoyao Ni, Yawei Wang, Qiuzan Zhong, Mei Li, Dandan Zhang, Yongjun Zhang, Xinghui Qiu
Summary: This study found a wealth of nucleotide variations in the cotton bollworm populations in China, but no previously reported kdr-conferring amino acid replacements or other non-synonymous mutations were observed. However, the CYP337B3 gene was widely present in these populations, indicating its prevalent role in pyrethroid resistance development.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Xin Wu, Liang Hong
Summary: Calmodulin (CaM) is a small protein that serves as a ubiquitous signal transducer, regulating neuronal plasticity, muscle contraction, and immune response. It interacts with ion channels and plays regulatory roles in cellular electrophysiology. Mutations in CaM-binding IQ domain can lead to various diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Entomology
Prashant K. Mallick, Ankita Sindhania, Toshi Gupta, Dhirendra P. Singh, Seema Saini, Om P. Singh
Summary: This study aims to determine the presence of kdr mutations in the population of Indian head lice. By sequencing the genome of head louse samples collected from different regions of India, four kdr mutations were detected. Among them, L1014F is the most commonly reported mutation associated with the kdr trait in insect taxa.
MEDICAL AND VETERINARY ENTOMOLOGY
(2023)
Article
Environmental Sciences
Courtney Y. Fung, Kun Yan Zhu, Kaley Major, Helen C. Poynton, Kara E. Huff Hartz, Gary Wellborn, Michael J. Lydy
Summary: Chronic exposure to pyrethroid insecticides can lead to evolution of resistance in non-target aquatic species. The mechanisms of resistance in the Hyalella azteca were found to involve mutations in the voltage-gated sodium channel and enhanced enzyme-mediated detoxification. The resistance was primarily attributed to a heritable mutation at the VGSC locus, with additional tolerance conferred through detoxification pathways.
ENVIRONMENTAL POLLUTION
(2021)
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: A piggyBac-based germline transformation system was established in the fall armyworm, Spodoptera frugiperda, a global agricultural pest. Tissue-specific promoters were identified and used to drive the expression of transgenes, resulting in tissue-specific overexpression. Overexpression of certain genes in the midgut was found to confer insecticide resistance.
JOURNAL OF PEST SCIENCE
(2023)
Article
Multidisciplinary Sciences
Sharath Chandra Gaddelapati, Najla M. Albishi, Ramesh Kumar Dhandapani, Subba Reddy Palli
Summary: This study identified 10 genes coding for HDACs in the yellow fever mosquito and determined their role in larval development using RNA interference. The findings suggest that HDAC3 plays a crucial role in larval development and knockdown of HDAC3 leads to larval death and affects midgut size.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Infectious Diseases
Anna R. Pasternak, Subba R. Palli
Summary: Lyme disease, the most common tick-borne illness in the United States, is increasing in prevalence each year. A study conducted in Kentucky, a non-endemic area, revealed a more than 300% increase in reported human cases from 2010 to 2019. Monitoring the distribution of infected ticks is crucial for effective prevention strategies and disease surveillance.
TICKS AND TICK-BORNE DISEASES
(2022)
Article
Entomology
Xien Chen, Subba Reddy Palli
Summary: This study reports the establishment of multiple transgenic CRISPR/Cas9-based genome editing methods in the global agricultural pest, fall armyworm, Spodoptera frugiperda. Fluorescent proteins suitable for screening for transgenic fall armyworm were identified. The use of U6-tRNA-sgRNA system for sgRNA expression was found to be more efficient in inducing target gene knockout. These methods provide valuable tools for gene editing and functional genomics studies in the fall armyworm and other lepidopteran pests.
JOURNAL OF PEST SCIENCE
(2023)
Editorial Material
Agronomy
Yulin Gao, Andrei Alyokhin, Ralf Nauen, Raul N. C. Guedes, Subba Reddy Palli
PEST MANAGEMENT SCIENCE
(2022)
Article
Genetics & Heredity
Guang-Hua Luo, Xi-En Chen, Yao-Yu Jiao, Guan-Heng Zhu, Ru Zhang, Ramesh Kumar Dhandapani, Ji-Chao Fang, Subba Reddy Palli
Summary: This study reveals that the SoxC gene plays a crucial role in the regulation of insect ecdysis, and is associated with the expression of neuropeptide genes such as EH.
FRONTIERS IN GENETICS
(2022)
Review
Entomology
Marc Kenis, Giovanni Benelli, Antonio Biondi, Paul-Andre Calatayud, Roger Day, Nicolas Desneux, Rhett D. Harrison, Darren Kriticos, Ivan Rwomushana, Johnnie van den Berg, Francois Verheggen, Yong-Jun Zhang, Lakpo Koku Agboyi, Regis Besmer Ahissou, Malick N. Ba, Julio Bernal, Adeney de Freitas Bueno, Yves Carriere, Geraldo Andrade Carvalho, Xue-Xin Chen, Lizette Cicero, Hannalene du Plessis, Regan Early, Patrick Fallet, Komi K. M. Fiaboe, Dnyaneshwar M. Firake, Georg Goergen, Astrid T. Groot, Raul N. C. Guedes, Ankita Gupta, Gao Hu, Fn Huang, Lara R. Jaber, Edi A. Malo, Christina B. McCarthy, Robert L. Meagher, Samira Mohamed, David Mota Sanchez, Rodney N. Nagoshi, Nicolas Negre, Saliou Niassy, Noboru Ota, Casper Nyamukondiwa, Celso Omoto, Subba Reddy Palli, Roman Pavela, Ricardo Ramirez-Romero, Julio C. Rojas, Sevgan Subramanian, Bruce E. Tabashnik, Wee Tek Tay, Eduardo Gabriel Virla, Su Wang, Trevor Williams, Lian-Sheng Zang, Lisheng Zhang, Kongming Wu
Summary: This article provides a comprehensive review of the fall armyworm, including its biology, worldwide status and spread, management strategies, and recommendations for sustainable management in invaded regions.
ENTOMOLOGIA GENERALIS
(2023)
Article
Cell Biology
Surjeet Kumar Arya, Cynthia L. Goodman, David Stanley, Subba Reddy Palli
Summary: This article presents an online database describing known cell lines from agricultural pests in the orders Coleoptera, Diptera, Hemiptera, Hymenoptera, and Lepidoptera. The database includes 719 cell lines from 86 species. The information for the database was obtained from previous compilations, internet literature searches, and cell line depositories.
IN VITRO CELLULAR & DEVELOPMENTAL BIOLOGY-ANIMAL
(2022)
Article
Biochemistry & Molecular Biology
Xien Chen, Subba Reddy Palli
Summary: This study identified JHREs and developed a JHRE-reporter cell platform to screen for JH analogs. It found species-specific functionality of JHREs in different cells. These findings provide a potential tool for screening insecticides to control pests like the fall armyworm.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2022)
Article
Agronomy
Xingeng Wang, Jessica Faucher, Ramesh Kumar Dhandapani, Jian J. Duan, Subba Reddy Palli
Summary: This study investigated the impact of dsRNA-based insecticides on the survival and behavior of a parasitoid wasp. The results showed that direct injection of dsRNA did not affect the wasp's survival, but it indirectly affected the suitability and detectability of host larvae.
PEST MANAGEMENT SCIENCE
(2023)
Article
Infectious Diseases
Anna R. Pasternak, Subba R. Palli
Summary: Kentucky experiences a high incidence of ehrlichiosis, primarily transmitted by the lone star tick. Surveillance of tick distribution and pathogen detection is crucial for monitoring the expansion of this important tick vector and the increase in tick-borne diseases.
TICKS AND TICK-BORNE DISEASES
(2023)
Article
Multidisciplinary Sciences
Mengdi Zhang, Xinyue Cheng, Runmao Lin, Bingyan Xie, Ralf Nauen, Silvia I. Rondon, Jorge A. Zavala, Subba Reddy Palli, Suhua Li, Xingyao Xiong, Wenwu Zhou, Yulin Gao
Summary: This study reports the genome assembly of the potato tuberworm at the chromosomal level, providing a significant resource for understanding the genetic basis of this insect and promoting its integrative management in the future.
Article
Multidisciplinary Sciences
Heng Jiang, Xiangkun Meng, Nan Zhang, Huichen Ge, Jiaping Wei, Kun Qian, Yang Zheng, Yoonseong Park, Subba Reddy Palli, Jianjun Wang
Summary: This study found that deltamethrin-induced oxidative stress activates AMPK, promoting the nuclear translocation of CncC through phosphorylation. CncC acts as a transcription activator of detoxification genes and regulates the expression of genes involved in ecdysteroid biosynthesis and juvenile hormone degradation. This results in increased ecdysteroid levels, decreased juvenile hormone titer, and down-regulated vitellogenin gene expression.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Article
Biochemistry & Molecular Biology
Jinmo Koo, Dhandapani Gurusamy, Subba Reddy Palli
Summary: There is limited success in using exogenous small interfering RNA (siRNA) or small hairpin RNA (shRNA) to trigger RNA interference (RNAi) in insects. Instead, long double-stranded RNA (dsRNA) is used to induce gene knockdown in insects. In this study, the potency of si/sh RNAs and dsRNA was compared in Colorado potato beetle (CPB) cells. CPB cells showed a highly efficient RNAi response to dsRNA, but si/sh RNAs were inefficient in triggering RNAi in CPB cells.
ARCHIVES OF INSECT BIOCHEMISTRY AND PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yaoyu Jiao, Subba Reddy Palli
Summary: m6A methylation is a common modification of mRNA, which regulates multiple stages of mRNA processing. In this study, the role of m6A modification in insect development was explored using the red flour beetle as a model. Knockdown of m6A writers and readers disrupted ecdysis during eclosion, sterilized the insects, and affected egg laying and embryonic development.
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
(2023)