Journal
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
Volume 63, Issue 32, Pages 7134-7142Publisher
AMER CHEMICAL SOC
DOI: 10.1021/acs.jafc.5b03196
Keywords
Glycine max; Glycine sofa; 2D gel electrophoresis; MALDI-TOF/TOF; seed-storage proteins; protamine sulfate precipitation method
Funding
- Next-Generation Bio Green 21 Program [PJ011070032015]
- National Agenda Programs for Agricultural R&D of Rural Development Administration, Republic of Korea [PJ01002002]
- Rural Development Administration (RDA), Republic of Korea [PJ011070032015] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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This study develops differential protein profiles of soybean (Glycine max) seeds (cv. Saedanbaek and Daewon) varying in protein (47.9 and 39.2%) and oil (16.3 and 19.7%) content using protamine sulfate (PS) precipitation method coupled with a 2D gel electrophoresis (2DGE) approach. Of 71 detected differential spots between Daewon and Saedanbaek, 48 were successfully identified by MALDI-TOF/TOF. Gene ontology analysis revealed that up-regulated proteins in Saedanbaek were largely associated with nutrient reservoir activity (42.6%), which included mainly seed-storage proteins (SSPs; subunits of glycinin and beta-conglycinin). Similar results were also obtained in two cultiyars of wild soybean (G. sofa cv. WS22 and WS15) differing in protein content. Western blots confirmed higher accumulation of SSPs in protein-rich Saedanbaek. Findings presented and discussed in this study highlight a possible involvement of the urea cycle for increased accumulation of SSPs and hence the higher protein content in soybean seeds.
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