Article
Biochemical Research Methods
Christian A. Rosales, Samuel W. J. Shields, Chelsey L. J. Aulenback, Gazmend Elezi, Karl V. Wasslen, Peter J. Pallister, Kym F. Faull, Jeffrey M. Manthorpe, Jeffrey C. Smith
Summary: This article introduces a method for detecting glyphosate (GLY) and its derivatives in the environment using chemical derivatization and high-performance liquid chromatography-mass spectrometry (HPLC-MS). By using in situ trimethylation enhancement with diazomethane (iTrEnDi), GLY and its derivatives were successfully transformed into permethylated products, improving the detection sensitivity. The results showed significant sensitivity improvement compared to previous derivatization techniques, and residues of GLY and its derivatives on the surface of soybeans sprayed with GLY were detected. In summary, the iTrEnDi method improves low proton affinity and chromatographic retention issues, enhances HPLC-MS sensitivity, and enables the detection of elusive analytes such as GLY and its derivatives in agricultural systems.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Food Science & Technology
Cheng Li, Zhiqian Liu, Leah Marett, Jennie Pryce, Simone Rochfort
Summary: Milk is a rich source of lipids, and LC-MS is the predominant technique for lipid identification and quantification. However, no standard methods are currently available for the quantification of milk phospholipids. This study aims to examine the factors influencing the quantification outcome of bovine milk phospholipids and discuss the pros and cons of current LC-MS methods.
Article
Biochemical Research Methods
Martins Jansons, Iveta Pugajeva, Vadims Bartkevics, Hark Bahadur Karkee
Summary: A simple procedure has been developed for the extraction, pre-column derivatisation and mass spectrometric detection of glyphosate and other polar and acidic pesticides. The proposed procedure showed good method performance, with improvements in peak shape, peak intensity and calibration curve slope compared to conventional methods.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Environmental Sciences
Marisa A. Wirth, Lars Longwitz, Marion Kanwischer, Peter Gros, Peter Leinweber, Thomas Werner
Summary: Stable isotope labeling of pollutants is important for studying their environmental fate. A synthetic pathway to the labeled metabolite AMPA-N-15 was developed for the commonly used herbicide glyphosate, enabling its accurate detection and quantitation in soil extracts. The newly developed analysis concepts will facilitate future research on the environmental behavior of glyphosate.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2021)
Article
Biochemical Research Methods
L. Perez-Mayan, G. Castro, M. Ramil, R. Cela, I Rodriguez
Summary: Two different analytical methodologies were evaluated for detecting GLY and AMPA residues in wine samples, with one utilizing derivative transformation and the other direct determination. The use of molecularly imprinted sorbent for extraction and a strong anionic exchange LC column for direct determination proved to be effective in overcoming the challenges presented by the wine matrix.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Zhiyong Zhao, Tingting Fan, E. Hengchao, Yanmei Zhang, Xiaobei Li, Xianli Yang, Enjing Tian, Ailiang Chen, Xiaoyan Zhao, Changyan Zhou
Summary: Mushroom poisoning is a serious food safety and public health issue in certain parts of the world, with high morbidity and mortality rates. This study developed a new method for simultaneous detection of four mushroom toxins in mushroom and urine samples. The method was validated for sensitivity, recovery, and precision, and successfully applied to cooked mushroom samples and vomitus, providing valuable materials for identifying mushroom poisoning caused by these toxins.
Article
Biochemistry & Molecular Biology
Zhiqian Liu, Simone Rochfort
Summary: Regioisomers of triacylglycerols in milk have differential effects on human absorption, but quantifying their ratios remains challenging due to the lack of easy chromatographic separation techniques. This study confirmed the feasibility of using fragment ion intensity ratio to determine regioisomer pairs and demonstrated the ability of C30 stationary phase in resolving interfering isomers. The research proposed a novel algorithm to consider double bond isomers and eliminate interference from isobaric ions, presenting a simple LC-MS method suitable for genetic analysis of milk samples.
Article
Environmental Sciences
Garth Campbell, Andrea Mannetje, Samuel Keer, Geoff Eagleshama, Xianyu Wang, Chun-Yin Lin, Peter Hobson, Leisa-Maree Toms, Jeroen Douwes, Kevin V. Thomas, Jochen F. Mueller, Sarit Kaserzon
Summary: This study analyzed urine samples from the Australia and New Zealand region and obtained data on the concentrations of glyphosate and its degradation product aminomethylphosphonic acid (AMPA). The results showed that glyphosate was detected in 8% of the urine samples from the Australian general population and in 96% of urine samples from New Zealand farmers. This indicates a widespread presence of glyphosate in the population, with higher detections among farmers.
SCIENCE OF THE TOTAL ENVIRONMENT
(2022)
Review
Chemistry, Analytical
Priscilla Rocio-Bautista, David Moreno-Gonzalez, Ana B. Martinez-Piernas, Juan F. Garcia-Reyes, Antonio Molina-Diaz
Summary: This review discusses the application of liquid chromatography-mass spectrometry (LC-MS) technique in the determination of glyphosate (Gly) and related compounds. The sample preparation methods and novel liquid chromatography/mass spectrometry approaches are introduced, and the analytical challenges, strengths, and drawbacks are critically discussed.
TRENDS IN ENVIRONMENTAL ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Xinyue Xu, Huaitian Cui, Jiaxin Xu, Zhiheng Yuan, Xiaoqing Liu, Xiangrong Fan, Jun Li, Danshi Zhu, He Liu
Summary: Fermentation of soy yogurt with different combinations of probiotic micro-organisms had varying effects on yogurt quality, texture, flavor, and soy isoflavone content. The use of Danisco probiotic starter resulted in the best overall yogurt quality, including shorter fermentation time, better water holding capacity, and higher sensory scores.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Engineering, Environmental
Pankaj Bhatt, Tushar Joshi, Kalpana Bhatt, Wenping Zhang, Yaohua Huang, Shaohua Chen
Summary: The widespread use of glyphosate poses a threat to living organisms, but microbes can degrade it with specific enzymes, including glyphosate oxidoreductase and C-P lyase. This study used molecular docking and dynamics simulations to investigate how glyphosate interacts with these enzymes and maintains stability in aqueous environments. The results provide insights into the role of these enzymes in bioremediation of glyphosate.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemical Research Methods
Olivier Delhomme, Anais Hernandez, Supansa Chimjarn, Colette Bertrand, Marjolaine Bourdat-Deschamps, Clementine Fritsch, Celine Pelosi, Sylvie Nelieu, Maurice Millet
Summary: A new analytical methodology was developed to quantify glyphosate, aminomethylphosphonic acid, and glufosinate in soil and earthworms. The method involved extraction, derivatization, and purification steps, followed by quantification using LC/MS/MS. The optimized method provided low limit of quantification values and was successfully applied in natural samples.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Food Science & Technology
Emad Karrar, Isam A. Mohamed Ahmed, Thom Huppertz, Fatih Oz, Wei Wei, Xingguo Wang
Summary: This study characterized the triacylglycerols (TAGs) in milk fat from cow, yak, donkey, goat, and camel using UPLC-Q-TOF-MS. A total of 47, 52, 61, 75, and 87 TAG species were identified in camel, goat, donkey, cow, and yak milk fat, respectively. The most abundant TAG species varied among the different milk fats. This work contributes to a better understanding of TAGs in milk from various species.
INTERNATIONAL DAIRY JOURNAL
(2022)
Article
Chemistry, Analytical
Yi Dai, Chu-Bo Qi, Yang Feng, Qing-Yun Cheng, Fei-Long Liu, Ming-Yu Cheng, Bi-Feng Yuan, Yu-Qi Feng
Summary: The discovery of dynamic and reversible modifications in RNA has expanded their functional repertoires, offering new insight into biological processes. A novel chemical derivatization strategy combined with mass spectrometry analysis has been developed to sensitively and simultaneously determine uridine thiolation and hydroxylation modifications in eukaryotic RNA, enhancing detection sensitivity by up to 339-fold. This method has been used to identify a new modification of 5-hydroxyuridine (ho(5)U) in mammalian cell small RNAs, demonstrating superior capability in detecting low-abundance RNA modifications and potentially leading to the discovery of new modifications in RNA.
ANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Applied
Justine M. Cruz, Jacolin A. Murray
Summary: The study aimed to optimize and validate a method for extraction and analysis of glyphosate and AMPA, identifying different levels of glyphosate in oat products and finding candidate RMs, with a preliminary stability study showing glyphosate is stable in oat material for six months.