Article
Chemistry, Applied
Xu Pang, Xiuhua Liu, Liangrui Peng, Zhijun Chen, Jing Qiu, Xin Su, Changyuan Yu, Jiguang Zhang, Rui Weng
Summary: This study developed a sensitive and reliable multi-residue pesticide analysis method for beef using 129 pesticides, with satisfactory sensitivity and accuracy demonstrated during method validation. The method was optimised using an orthogonal array design, significantly enhancing scientific rigor and simplifying the process.
Article
Environmental Sciences
Zahra Eslami, Vahideh Mahdavi, Behrouz Tajdar-Oranj
Summary: The study validated an analytical procedure for multiresidue analysis of pesticides in dates using the QuEChERS approach. Results showed satisfactory validation outcomes, with pesticide residues in 92% of samples below national MRLs, indicating low health risk to humans from consuming dates.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Chemistry, Applied
Jesus M. Gonzalez-Jartin, Ines Rodriguez-Canas, Amparo Alfonso, Maria J. Sainz, Mercedes R. Vieytes, Ana Gomes, Isabel Ramos, Luis M. Botana
Summary: A simple method for quantifying 40 mycotoxins in milk was developed using QuEChERS extraction and UHPLC-MS/MS detection, achieving low LOQs, high recoveries, and acceptable performance characteristics in line with European regulations. The method successfully quantified mycotoxins in raw milk, highlighting the high occurrence of beauvericin and enniatins in small amounts.
Article
Food Science & Technology
Jesus M. Gonzalez-Jartin, Vanesa Ferreiroa, Ines Rodriguez-Canas, Amparo Alfonso, Maria J. Sainz, Olga Aguin, Mercedes R. Vieytes, Ana Gomes, Isabel Ramos, Luis M. Botana
Summary: Maize and grass silages are important for ruminant livestock, but the presence of fungi and mycotoxins can impact the quality of silage and subsequently, milk. This study in Portugal found common fungal species and analyzed their mycotoxin profiles. Fusarium mycotoxins were detected at low levels, while Penicillium and Aspergillus toxins were less prevalent in the silages studied.
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY
(2022)
Article
Chemistry, Applied
Zahra Eslami, Vahideh Mahdavi, Alireza Akbarzadeh Mofrad
Summary: This research analyzed pesticide residues in ready-to-use barberry samples from hypermarkets in Iran using QuEChERS approach and UHPLC-MS/MS technology. The non-carcinogenic health risk assessment based on Monte Carlo Simulation method was conducted. The results showed that 48.5% of samples contained pesticide residues, with approximately 14% exceeding the MRL values. The HQ and HI values for adults and children consuming barberry were within a certain range.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2022)
Article
Chemistry, Applied
Yingying Fan, Fengjuan Liu, Weizhong He, Qiaomei Qin, Dongqiang Hu, Aibo Wu, Weibo Jiang, Cheng Wang
Summary: An improved method was developed for the detection of mycotoxins in fruit samples from Xinjiang, providing accurate data and revealing significant contamination with mycotoxins, with the highest levels of three Alternaria toxins found in jujube, wolfberries, and raisins.
Article
Chemistry, Analytical
Fajun Tian, Chengkui Qiao, Caixia Wang, Jing Luo, Linlin Guo, Tao Pang, Jun Li, Ruiping Wang, Rongli Pang, Hanzhong Xie
Summary: A sensitive method has been developed for the determination of three new pesticides in cereals, vegetables, and fruits samples, showing good linearities and accuracy. This method provides a reliable tool for monitoring these pesticides.
MICROCHEMICAL JOURNAL
(2021)
Article
Chemistry, Analytical
I Vergara-Luis, J. C. Baez-Millan, I Baciero, B. Gonzalez-Gaya, M. Olivares, O. Zuloaga, A. Prieto
Summary: The extensive use of antibiotics in agriculture has resulted in the presence of residual drugs in commonly consumed vegetables, which could potentially harm human health and promote the spread of antibiotic-resistant genes. In this study, two accurate sample preparation procedures were developed and validated for the simultaneous analysis of sulfonamides (SAs) and tetracyclines (TCs) in popular European vegetables. The evaluated protocols, which utilized QuECHERS for extraction and subsequent clean-up by SPE or dispersive SPE, demonstrated good recovery and precision for most analytes. These methods were applied to market vegetable samples and only sulfamethazine (SMZ) and sulfapyridine (SPD) were detected in lettuce.
Article
Biochemical Research Methods
Bowen Huang, Adabelle Ong, Wenli Wang, Yu He, Yongjun Xiao
Summary: The detection and understanding of emerging contaminants (ECs) pose new challenges to water pollution control. Triclosan (TCS), an emerging contaminant widely found in the environment, is a commonly used antibacterial agent. Microdialysis (MD), a sampling technique, combined with UHPLC-QTOF/MS analysis, was used to identify endogenous metabolites as biomarkers of TCS exposure in living crucian carp. The quantification of these biomarkers using UHPLC-MS/MS provided continuous monitoring of the effect of TCS on endogenous metabolites, contributing to a better understanding of its toxicological effect.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Agriculture, Dairy & Animal Science
Jesus M. Gonzalez-Jartin, Amparo Alfonso, Maria J. Sainz, Mercedes R. Vieytes, Luis M. Botana
Summary: A new method for multi-mycotoxin analysis was developed, based on QuEChERS extraction and UHPLC-MS/MS detection, with high recoveries and low LOQs. The study also discovered a new physical method using glass spheres to remove mycotoxins from raw materials and feedstuffs, showing a removal rate of up to 28%.
ANIMAL FEED SCIENCE AND TECHNOLOGY
(2021)
Article
Engineering, Environmental
Yanli Man, Marianne Stenrod, Chi Wu, Marit Almvik, Roger Holten, Jihong Liu Clarke, Shankui Yuan, Xiaohu Wu, Jun Xu, Fengshou Dong, Yongquan Zheng, Xingang Liu
Summary: The laboratory experiments revealed that difenoconazole undergoes oxidation, dechlorination, and hydroxylation reactions in the environment, leading to the formation of various transformation products. Four novel transformation products showed lower acute toxicity compared to difenoconazole, but were predicted to be chronically very toxic to fish, posing a potential threat to aquatic ecosystems.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Chemistry, Analytical
Lichun Li, Yi Yin, Guangming Zheng, Shugui Liu, Cheng Zhao, Wenping Xie, Lisha Ma, Qi Shan, Xiaoxin Dai, Linting Wei
Summary: A sensitive method for determining nine herbicides in sediments and aquatic products was developed using QuEChERS and UHPLC-MS/MS, with satisfactory recoveries and linear calibration curves. While the residues of herbicides in paddy fish did not harm humans in the rice-fish co-culture system, there were ecological risks to fish, daphnia, and algae in the system.
MICROCHEMICAL JOURNAL
(2021)
Article
Agriculture, Multidisciplinary
Yiming Zhang, Lanlan Zhan, Quan Wen, Yulin Feng, Yun Luo, Ting Tan
Summary: This study discovered that taxifolin is able to effectively trap methylglyoxal (MGO), an important precursor of advanced glycation end products (AGEs), by forming MGO adducts in vitro and in vivo. Quantification studies showed that taxifolin and its metabolites can dose-dependently trap MGO. Similar results were observed in diabetic mice.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Chemistry, Analytical
Noha F. El Azab
Summary: In this study, a LC-MS/MS approach was developed to detect and quantify certain COVID-19 candidate drugs in rat plasma. The method was validated according to US FDA criteria and showed satisfactory recoveries, low detection limits, and good precision and accuracy.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Applied
Vahideh Mahdavi, Zahra Eslami, Gholamreza Golmohammadi, Behrouz Tajdar-oranj, Arnavaz Keikavousi Behbahan, Amin Mousavi Khaneghah
Summary: The study analyzed 88 pesticides in 34 Iranian saffron samples using a miniaturized QuEChERS approach and UHPLC-MS/MS, finding that 3.4% of the samples were contaminated by at least one pesticide. However, the majority of samples had pesticide residue levels much below the EU MRLs, with the health risk assessment indicating that Iranian saffron samples are generally safe for consumption by adults and children.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2021)
Article
Agriculture, Multidisciplinary
Verena Gratl, Sonja Sturm, Elena Zini, Thomas Letschka, Marco Stefanini, Silvia Vezzulli, Hermann Stuppner
Summary: The study comparing the polyphenolic profiles of hybrid grapevine varieties from international breeding programs and private initiatives in the same vineyard showed similar overall profiles but some variations in specific compounds. This research will help in selecting promising candidates for further breeding programs aimed at developing highly resistant grape varieties with improved sensory characteristics.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Plant Sciences
Sophia Mayr, Simon Strasser, Christian G. Kirchler, Florian Meischl, Stefan Stuppner, Krzysztof B. Bec, Justyna Grabska, Sonja Sturm, Hermann Stuppner, Michael A. Popp, Gunther K. Bonn, Christian W. Huck
Summary: Near-infrared spectroscopy was used to analyze the content of silymarin and individual flavonolignans in milk thistle seeds. The benchtop device NIRFlex N-500 showed the best performance, while quantum chemical simulation of spectra helped to understand the impact of chemical and physical properties on analysis. Miniaturized near-infrared spectroscopy proved to be capable of rapid and nondestructive quantification of silymarin and flavonolignans in whole milk thistle seeds.
Review
Medical Laboratory Technology
Stein Bergan, Merce Brunet, Dennis A. Hesselink, Kamisha L. Johnson-Davis, Pawel K. Kunicki, Florian Lemaitre, Pierre Marquet, Mariadelfina Molinaro, Ofelia Noceti, Smita Pattanaik, Tomasz Pawinski, Christoph Seger, Maria Shipkova, Jesse J. Swen, Teun van Gelder, Raman Venkataramanan, Eberhard Wieland, Jean-Baptiste Woillard, Tom C. Zwart, Markus J. Barten, Klemens Budde, Maja-Theresa Dieterlen, Laure Elens, Vincent Haufroid, Satohiro Masuda, Olga Millan, Tomoyuki Mizuno, Dirk J. A. R. Moes, Michael Oellerich, Nicolas Picard, Linda Salzmann, Burkhard Toenshoff, Ron H. N. van Schaik, Nils Tore Vethe, Alexander A. Vinks, Pierre Wallemacq, Anders Asberg, Loralie J. Langman
Summary: This report provides an updated and comprehensive presentation of consensus on personalized treatment with MPA, including topics such as analytical criteria, pharmacogenetics, biomarker development, and practical intervention aspects. Graded recommendations on target ranges for selected topics, such as the limited sampling strategies for MPA area under the curve, are included to aid in personalized dosing.
THERAPEUTIC DRUG MONITORING
(2021)
Article
Biochemistry & Molecular Biology
Sonja Sturm, Christina Hoegner, Christoph Seger, Hermann Stuppner
Summary: The study investigated the in vitro metabolism of vitetrifolin D using HPLC-DAD-QTOF-MS and HPLC-SPE-NMR, identifying 27 phase I and phase II metabolites, with structural characterization of nine phase I metabolites. By avoiding preparative isolation of metabolites, the study demonstrated the successful performance of in vitro metabolism studies using hyphenated instrumental analysis methodologies even with limited substance availability.
Article
Agriculture, Multidisciplinary
Nora Gibitz-Eisath, Christoph Seger, Stefan Schwaiger, Sonja Sturm, Hermann Stuppner
Summary: This study presents the first ultra-high performance supercritical fluid chromatography-diode array detector (UHPSFC-DAD) assay for simultaneous quantitation of secoiridoids, iridoids, xanthones, and xanthone glycosides in Gentiana lutea L. The method validation confirmed the assay's sensitivity, linearity, precision, and accuracy. The practical applicability was proven by the analysis of root specimens and commercial liquid preparations.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2022)
Article
Medical Laboratory Technology
Linda Salzmann, Janik Wild, Neeraj Singh, Tobias Schierscher, Franziska Liesch, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: This study developed a reference measurement procedure for gabapentin, using quantitative nuclear magnetic resonance (qNMR) spectroscopy to determine the absolute content of standard materials and isotope dilution-liquid chromatography-tandem mass spectrometry (ID-LC-MS/MS) to accurately measure the concentration of gabapentin in serum and plasma. The analytical protocol showed good selectivity, precision, and accuracy, allowing for standardization of routine tests and reliable evaluation of clinical samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Christoph Seger, Anja Kessler, Judith Taibon
Summary: For molecules that can be well described metrologically and recorded analytically, traceability to metrologically sound foundation is necessary. National Metrology Institutes (NMIs) play a leading role in the establishment of traceability chains. If NMIs cannot take up this task, alternative approaches must be considered, such as traceability initiatives by the in vitro device industry or academia adhering to quality standards of NMIs. Adherence to ISO procedure 15193 is essential for the establishment of reference measurement procedures (RMPs), and thorough CRM characterization and JCTLM listing are crucial for both RMPs and CRMs.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Valentin Braun, Hermann Stuppner, Lorenz Risch, Christoph Seger
Summary: Steroid analysis in clinical laboratories is currently dominated by immunoassays, but liquid chromatography coupled to mass spectrometry (LC-MS/MS) has been proven to be a more capable tool with better sensitivity, specificity, and the ability to analyze multiple steroids and metabolites simultaneously, providing more reliable and comprehensive diagnostic information for endocrinologists.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Cell Biology
Christoph Seger, Sonja Sturm
Summary: NMR spectroscopy is essential for determining the structural information of analytes from natural sources in liquid state. While mass spectrometry can differentiate and group biomarkers, the structural characterization often requires the use of NMR spectroscopy.
Article
Cell Biology
Valentin Braun, Hermann Stuppner, Christoph Seger
Summary: Screening for potential interferences in multi-analyte LC-MS/MS method development should not only cover substances of the same class but also include commonly prescribed drugs. In this study, two newly discovered potential interferences from non-steroidal drugs, paroxetine (PX) and alpha-hydroxytriazolam (alpha-OH-TZM), were identified as interferences to 17-hydroxyprogesterone (17P) and aldosterone (ALDO) respectively. These interferences should be considered clinically relevant as PX and TZM are frequently prescribed drugs with therapeutic ranges far exceeding the reference ranges of 17P or ALDO.
Article
Medical Laboratory Technology
Linda Salzmann, Tino Spescha, Neeraj Singh, Tobias Schierscher, Martina Bachmann, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: In this study, an isotope dilution (ID)-liquid chromatography-tandem mass spectrometry (LC-MS/MS)-based candidate reference measurement procedure (RMP) was developed for the measurement of lamotrigine in human serum and plasma. The method provides traceability and reliability for standardization of routine assays and evaluation of clinical samples.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Anja Kobel, Tobias Schierscher, Neeraj Singh, Linda Salzmann, Franziska Liesch, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: Developed an isotope dilution-liquid chromatography-tandem mass spectrometry-based candidate reference measurement procedure (RMP) for levetiracetam quantification in human serum and plasma. The RMP was highly selective and specific, with no evidence of a matrix effect. Its expanded measurement uncertainty of <= 4.0% meets the clinical needs in levetiracetam monitoring. Utilized quantitative nuclear magnetic resonance spectroscopy (qNMR) to ensure metrological traceability to SI units for levetiracetam reference materials.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Medical Laboratory Technology
Linda Salzmann, Tino Spescha, Neeraj Singh, Anja Kobel, Vanessa Fischer, Tobias Schierscher, Friederike Bauland, Andrea Geistanger, Lorenz Risch, Christian Geletneky, Christoph Seger, Judith Taibon
Summary: A novel reference measurement procedure (RMP) was developed and validated for the quantification of topiramate in human serum and plasma. The method showed high analytical performance, fulfilling the requirements on measurement uncertainty, and is suitable for routine assay standardization and clinical sample evaluation.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
