Journal
ARCHIVES OF DERMATOLOGICAL RESEARCH
Volume 301, Issue 7, Pages 497-506Publisher
SPRINGER
DOI: 10.1007/s00403-009-0948-4
Keywords
Keratinocyte; Collagen lattice; Collagenase; Matrix metalloproteinase-1; Wound healing; E-cadherin; Vinculin
Categories
Funding
- United Stated Public Health Service [GM077724, GM080779]
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Healing of superficial skin wounds depends on the proliferation and migration of keratinocytes at the wound margin. When human epidermal keratinocytes were incubated on polymerized type I collagen, they rapidly attached and spread. The cells underwent a proliferative response and, over the subsequent 6-day period, covered the collagen surface with a monolayer of cells. When keratinocytes were plated on collagen that had been fragmented by exposure to matrix metalloproteinase-1 (MMP-1, collagenase-1), the cells attached as readily as to intact collagen but spread more slowly and less completely. Growth was reduced by approximately 50%. Instead of covering the collagen surface, the keratinocytes remained localized to the site of attachment. Keratinocytes on fragmented collagen expressed a more differentiated phenotype as indicated by a higher level of surface E-cadherin. Based on these findings, we suggest that damage to the underlying collagenous matrix may impede efficient keratinocyte function and retard wound closure.
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