Article
Immunology
Robert Hullsiek, Yunfang Li, Kristin M. Snyder, Sam Wang, Da Di, Antonella Borgatti, Chae Lee, Peter F. Moore, Cong Zhu, Chiara Fattori, Jaime F. Modiano, Jianming Wu, Bruce Walcheck
Summary: Researchers have cloned canine CD16A and CD64 and generated specific monoclonal antibodies. They have reported for the first time the expression patterns of these Fc gamma receptors on dog peripheral blood leukocytes. These findings pave the way for developing engineered canine NK cells or T cells with high affinity recombinant canine CD64 to enhance antibody-dependent cell-mediated cytotoxicity (ADCC).
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Immunology
Bruce D. Wines, Halina M. Trist, Sandra Esparon, Rachael E. Impey, Graham A. Mackay, Robert K. Andrews, Tatiana P. Soares da Costa, Geoffrey A. Pietersz, Ross Baker, P. Mark Hogarth
Summary: The activation of Fc gamma RIIa by monoclonal antibodies requires interaction with both the Fab and Fc regions simultaneously on the same receptor molecules.
FRONTIERS IN IMMUNOLOGY
(2021)
Review
Immunology
Kristina Zlatina, Sebastian P. Galuska
Summary: This review discusses the impact of glycosylation on the function of antibodies, specifically Ig, and how changes in glycosylation affect their functionality. It also explores the functionality of IgG glycosylation in different physiological states, such as aging, lactation, and pathophysiological processes. Additionally, it points out the current knowledge about Ig glycosylation in farm animals and the potential contributions of new advancements in vaccination to animal welfare.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Oncology
Satoko Kakiuchi-Kiyota, Thorsten Ross, Heidi Ackerly Wallweber, James R. Kiefer, Melissa M. Schutten, Adeyemi O. Adedeji, Hao Cai, Robert Hendricks, Sivan Cohen, Srividya Myneni, Luna Liu, Aaron Fullerton, Nicholas Corr, Lanlan Yu, Denise de Almeida Nagata, Shelly Zhong, Steven R. Leong, Ji Li, Rin Nakamura, Teiko Sumiyoshi, Jinze Li, Ayse Meric Ovacik, Bing Zheng, Mike Dillon, Christoph Spiess, Susanne Wingert, Erich Rajkovic, Kristina Ellwanger, Uwe Reusch, Andrew G. Polson
Summary: Despite the current incurability of multiple myeloma (MM), the novel treatment, RO7297089, shows potential as an effective and well-tolerated therapy. By targeting BCMA and CD16A, RO7297089 induces lysis of MM cells and has a favorable safety profile, as evidenced by in vitro cytokine release and animal studies.
Article
Immunology
Carina Lynn Gehlert, Pegah Rahmati, Ammelie Svea Boje, Dorothee Winterberg, Steffen Krohn, Thomas Theocharis, Elisa Cappuzzello, Anja Lux, Falk Nimmerjahn, Ralf J. Ludwig, Marta Lustig, Thies Roesner, Thomas Valerius, Denis Martin Schewe, Christian Kellner, Katja Klausz, Matthias Peipp
Summary: This study engineered a CD19 antibody to enhance effector cell-mediated killing and CDC activity. The dual optimized CD19 antibody showed improved ADCC, ADCP, and CDC compared to the non-engineered antibody. This finding is crucial for improving CD19-directed cancer immunotherapy.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Immunology
Lois Coenon, Martin Villalba
Summary: This review provides an overview of the latest strategies employed to improve antibody-dependent NK cell cytotoxicity, by enhancing the biological function of CD16a receptor.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Medicine, Research & Experimental
Kerry A. Whalen, Kavya Rakhra, Naveen K. Mehta, Alexander Steinle, Jennifer S. Michaelson, Patrick A. Baeuerle
Summary: The field of immuno-oncology has revolutionized cancer patient care, but there are still challenges with current therapeutic approaches targeting T cells. Engaging the innate immune system, especially natural killer (NK) cells, has emerged as a promising modality in immunotherapy. This review focuses on therapeutic strategies that selectively engage NK cells for cancer therapy, particularly targeting the activating receptors NKG2D and CD16A.
Article
Biotechnology & Applied Microbiology
Alessia Gravina, Grigol Tediashvili, Raja Rajalingam, Zoe Quandt, Chad Deisenroth, Sonja Schrepfer, Tobias Deuse
Summary: Engineered overexpression of CD64 protects cells from antibody-mediated killing, enhancing the efficacy of allogeneic cell therapeutics for cancer therapy or regenerative medicine.
NATURE BIOTECHNOLOGY
(2023)
Article
Immunology
Adrian Elter, Desislava Yanakieva, David Fiebig, Kerstin Hallstein, Stefan Becker, Ulrich Betz, Harald Kolmar
Summary: This study introduces a novel Fc-tamed antibody format, which reduces off-target toxicity by blocking the Fc gamma R-binding sites of antibodies, resulting in decreased activation and improved safety profiles.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Medicine, Research & Experimental
Andrew R. Crowley, Matthew R. Mehlenbacher, Mohammad M. Sajadi, Anthony L. DeVico, George K. Lewis, Margaret E. Ackerman
Summary: Antibody-mediated effector functions can unfold according to an associative model of IgG-Fcγ receptor (FcγR) interactions or a competing model of antibody allostery. Evidence supports the antibody allostery model, but it remains controversial.
Review
Biochemistry & Molecular Biology
Maxime Fieux, Sandra Le Quellec, Sophie Battier, Andre Coste, Bruno Louis, Caroline Giroudon, Mikail Nourredine, Emilie Bequignon
Summary: FcRn has been shown to efficiently transport biologics across the nasal epithelial barrier in multiple studies reviewed. Limited studies have evaluated the potential toxicity of intranasal delivery of biologics, but they all indicate its safety. Further research is needed to fully understand the pharmacokinetic and pharmacodynamic properties of biologics after intranasal delivery.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Immunology
Melissa M. Lemke, Robert M. Theisen, Emily R. Bozich, Milla R. McLean, Christina Y. Lee, Ester Lopez, Supachai Rerks-Ngarm, Punnee Pitisuttithum, Sorachai Nitayaphan, Sven Kratochvil, Bruce D. Wines, P. Mark Hogarth, Stephen J. Kent, Amy W. Chung, Kelly B. Arnold
Summary: This study uses an ordinary differential equation model to quantitatively assess the influence of IgG1 allotypes and Fc gamma R polymorphisms on IgG-Fc gamma RIIIa complex formation in vaccine-relevant settings. The results demonstrate that specific vaccine interventions can enhance IgG-Fc gamma RIIIa complex formation in different genetic backgrounds.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Multidisciplinary Sciences
Algirdas Grevys, Rahel Frick, Simone Mester, Karine Flem-Karlsen, Jeannette Nilsen, Stian Foss, Kine Marita Knudsen Sand, Thomas Emrich, Jens Andre Alexander Fischer, Victor Greiff, Inger Sandlie, Tilman Schlothauer, Jan Terje Andersen
Summary: The variable region sequences of IgG antibodies significantly impact their cellular uptake, recycling, and degradation, as well as their binding to FcRn. Differences in charge patch also modulate binding and transport properties. Fc-engineering may improve FcRn binding and overcome unfavorable charge patches for longer plasma half-life.
Article
Immunology
Ana Rita Cruz, Arthur E. H. Bentlage, Robin Blonk, Carla J. C. de Haas, Piet C. Aerts, Lisette M. Scheepmaker, Inge G. Bouwmeester, Anja Lux, Jos A. G. van Strijp, Falk Nimmerjahn, Kok P. M. van Kessel, Gestur Vidarsson, Suzan H. M. Rooijakkers
Summary: This study elucidates the molecular mechanism by which Staphylococcus aureus protein A (SpA) blocks IgG-mediated phagocytosis, showing that it inhibits the interaction of IgGs with FcgRs and FcRn.
JOURNAL OF IMMUNOLOGY
(2022)
Article
Medicine, Research & Experimental
Ian Wilkinson, Geoff Hale
Summary: By assembling a dataset of amino acid sequences of antibody-related therapeutics, this study provides insights into trends in antibody engineering and a guide to frequently tested designs.
Article
Nanoscience & Nanotechnology
Anna Ohradanova-Repic, Eugenia Nogueira, Ingrid Hartl, Andreia C. Gomes, Ana Preto, Eva Steinhuber, Vanessa Muehlgrabner, Marko Repic, Mario Kuttke, Alexander Zwirzitz, Marek Prouza, Miloslav Suchanek, Gordana Wozniak-Knopp, Vaclav Horejsi, Gernot Schabbauer, Artur Cavaco-Paulo, Hannes Stockinger
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2018)
Article
Multidisciplinary Sciences
Gordana Wozniak-Knopp, Gerhard Stadlmayr, Jan Walther Perthold, Katharina Stadlbauer, Mathias Gotsmy, Stefan Becker, Florian Rueker
Article
Biochemistry & Molecular Biology
Sylvia Dietrich, Alec W. Gross, Stefan Becker, Bjorn Hock, Gerhard Stadlmayr, Florian Ruker, Gordana Wozniak-Knopp
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS
(2020)
Article
Biochemistry & Molecular Biology
Flavio Sadio, Gerhard Stadlmayr, Katharina Stadlbauer, Maximilian Graef, Agnes Scharrer, Florian Rueker, Gordana Wozniak-Knopp
Article
Cell Biology
Stefan Vogt, Madhusudhan Reddy Bobbili, Gerhard Stadlmayr, Katharina Stadlbauer, Jorgen Kjems, Florian Ruker, Johannes Grillari, Gordana Wozniak-Knopp
Summary: Research has shown that by using the CD81 protein to recognize target antigens, the targeted delivery capability of EVs can be enhanced, and this has been validated in mammalian cells. Mutant sequences are reformatted into full-length CD81 and expressed in EVs produced by HeLa cells, showing improved uptake by specific antigens.
JOURNAL OF EXTRACELLULAR VESICLES
(2021)
Article
Biochemistry & Molecular Biology
Veronica Natale, Gerhard Stadlmayr, Filippo Benedetti, Katharina Stadlbauer, Florian Ruker, Gordana Wozniak-Knopp
Summary: Bispecific antibodies and antibody fragments have increasing importance in clinical applications for enhanced targeting selectivity and modulation of multiple cellular pathways. In this study, symmetrical bispecific parental antibodies were engineered to guide the formation of a controlled trispecific antibody with minimal point mutations. The resulting molecules exhibited good biophysical characteristics and retained the antigen reactivity and biological activity of the parental constructs.
BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Rodak, Gerhard Stadlmayr, Katharina Stadlbauer, Dominic Lichtscheidl, Madhusudhan Reddy Bobbili, Florian Rueker, Gordana Wozniak-Knopp
Summary: The study of IgE is important in the treatment of allergies and asthma, and therapeutic anti-IgE antibodies can intervene in its function by different mechanisms to reduce serum IgE levels. Bispecific antibody fusion proteins have shown promising results in eliciting cytotoxic T-cell-mediated killing through specific activation.
Article
Biology
Filippo Benedetti, Katharina Stadlbauer, Gerhard Stadlmayr, Florian Rueker, Gordana Wozniak-Knopp
Summary: This study constructed a novel antibody by combining two different monoclonal antibodies, leading to a rapid reduction in surface receptor levels of HER2-positive cells and demonstrating a specific anti-proliferative effect in vitro against these cells.
Article
Biochemistry & Molecular Biology
Katharina Stadlbauer, Peter Andorfer, Gerhard Stadlmayr, Florian Ruker, Gordana Wozniak-Knopp
Summary: Inhibition of complement activation by overexpressing complement-regulatory proteins can disguise cancer cells from the immune system, but may also reduce the efficacy of therapeutic antibodies. By selecting CD59-specific Fc fragments, a bispecific antibody was created that can induce higher specific cytotoxicity at a lower concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Aleksandra Rodak, Katharina Stadlbauer, Madhusudhan Reddy Bobbili, Oskar Smrzka, Florian Rueker, Gordana Wozniak Knopp
Summary: This study developed an antibody-drug conjugate that showed cytotoxicity for IgE-positive cells. The antibody had fast internalization and strict target specificity, providing a valuable starting point for the development of an anti-IgE cell-depleting agent.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Filippo Benedetti, Florian Stracke, Gerhard Stadlmayr, Katharina Stadlbauer, Florian Ruker, Gordana Wozniak-Knopp
Summary: Monoclonal antibodies can be engineered to have high affinity binding to two target antigens by introducing multiple binding sites through C(H)3 domain modifications. The bispecific antibodies with exchanged antigen-binding C(H)3 domains showed expected molecular composition, high expression levels, and retained high thermostability in laboratory conditions. These antibodies offer an alternative solution in positioning and valency of antigen binding sites.
BIOCHEMISTRY AND BIOPHYSICS REPORTS
(2021)
Article
Medicine, Research & Experimental
Michael R. Dyson, Edward Masters, Deividas Pazeraitis, Rajika L. Perera, Johanna L. Syrjanen, Sachin Surade, Nels Thorsteinson, Kothai Parthiban, Philip C. Jones, Maheen Sattar, Gordana Wozniak-Knopp, Florian Rueker, Rachael Leah, John McCafferty
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)