Article
Immunology
Gabriela Specker, Damian Estrada, Rafael Radi, Lucia Piacenza
Summary: This study demonstrates the protective effects of Trypanosoma cruzi mitochondrial peroxiredoxin (MPX) in macrophage infections and in response to nifurtimox (NFX) toxicity. MPX content increased after NFX treatment and overexpression of MPX enhanced resistance to the drug. MPX exhibits holdase activity and its overexpression was observed in temperature-challenged parasites. These findings suggest a protective role of MPX holdase activity against NFX toxicity.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Lucia Lopez, Maria Laura Chiribao, Magali C. Girard, Karina A. Gomez, Paula Carasi, Marisa Fernandez, Yolanda Hernandez, Carlos Robello, Teresa Freire, Maria Dolores Pineyro
Summary: The study showed that c-TXNPx induces the recruitment of IL-12/23p40-producing innate antigen-presenting cells and promotes a strong specific Th1 immune response, while c-TXNPxC52S does not. The presence of peroxidatic cysteine is essential for the peroxidase activity and quaternary structure of the protein.
Review
Biochemistry & Molecular Biology
Lara Vogelsang, Karl -Josef Dietz
Summary: The temporal and spatial patterns of reactive oxygen species (ROS) in cells and tissues determine the plant's response to various stresses. Recent progress in dynamic cell imaging probes and sensitive proteomics has provided important information on the cellular redox state. Thiol peroxidases play a central role in this process.
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Microbiology
Maria Dolores Pineyro, Maria Laura Chiribao, Diego G. Arias, Carlos Robello, Adriana Parodi-Talice
Summary: This study investigated the sensitivity and dynamics of Trypanosoma cruzi Prxs to overoxidation in vitro and in an infection context. The results showed that Prxs were overoxidized in parasites treated with oxidants, and the overoxidized forms remained present at least 24 hours after treatment. Overoxidation of Prxs was also observed in amastigotes residing in infected macrophages. Furthermore, the study found that m-TXNPx has a redox-state-dependent chaperone activity.
Article
Biochemistry & Molecular Biology
Michaela Nicole Hoehne, Lianne J. H. C. Jacobs, Kim Jasmin Lapacz, Gaetano Calabrese, Lena Maria Murschall, Teresa Marker, Harshita Kaul, Aleksandra Trifunovic, Bruce Morgan, Mark Fricker, Vsevolod V. Belousov, Jan Riemer
Summary: Hydrogen peroxide (H2O2) plays a key role in signaling at physiological levels, but can cause molecular damage at high concentrations. This study used a genetically encoded H2O2 sensor to investigate how mitochondria release H2O2 in intact cells. The researchers found that H2O2 is released by mitochondria directly at the surface of the organelle and in the bulk cytosol, and that gradient formation is controlled by cytosolic peroxiredoxins.
Article
Immunology
Lucio Rivera-Santiago, Ignacio Martinez, Ruben Arroyo-Olarte, Paulina Diaz-Garrido, Roberto I. Cuevas-Hernandez, Bertha Espinoza
Summary: This study aimed to describe the structural characteristics of TcMPX and compare it with other PRXs, as well as reposition the antibiotic Thiostrepton as a potential inhibitor molecule. The results showed a high structural similarity between TcMPX and human PRX3, and demonstrated that Thiostrepton could affect the function of TcMPX and reduce the proliferation of T. cruzi.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Chemistry, Analytical
Di-Hua Tian, Jun-Ru Liu, Si-Yuan Wang, Shuai Yan, Zuo-Hu Chai, Fang Dai, Shengxiang Zhang, Bo Zhou
Summary: This study developed a new boronate-based fluorescent probe for selective imaging of H2O2 over ONOO-. The probe utilizes a specific reaction to release a fluorescent substance, resulting in a ratiometric fluorescence response. By using this probe, the increased levels of H2O2 during cellular processes and in live animals were successfully visualized.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Biochemistry & Molecular Biology
Andree G. Pearson, Juliet M. Pullar, John Cook, Emma S. Spencer, Margreet CM. Vissers, Anitra C. Carr, Mark B. Hampton
Summary: Intravenous infusion of high dose vitamin C increases intracellular ascorbate levels in erythrocytes, leading to H2O2 generation and oxidation of Prx2. Further investigation is needed to understand the impact of increased erythrocyte Prx2 oxidation.
Review
Biochemistry & Molecular Biology
Izabela Sadowska-Bartosz, Grzegorz Bartosz
Summary: Peroxiredoxin 2 (Prdx2) is an abundant erythrocyte protein that acts as an antioxidant, removing hydrogen peroxide and other peroxides. It can also serve as a chaperone for hemoglobin and erythrocyte membrane proteins.
Article
Biochemistry & Molecular Biology
Yunfeng Yan, Xiaofei Huang, Yao Zhou, Jingyi Li, Feiyun Liu, Xueying Li, Xiaotao Hu, Jing Wang, Lingyan Guo, Renning Liu, Naoki Takaya, Shengmin Zhou
Summary: In Aspergillus nidulans, the peroxiredoxin (PrxA) plays a crucial role in protecting dormant conidia against high levels of H2O2, while catalase (CatB) becomes the main antioxidant during the mature hyphae stage. CatB needs to be secreted to function, and this process is limited to the mature hyphae stage.
Article
Food Science & Technology
Ming-Hsun Shen, Rakesh K. Singh
Summary: This study investigated the effect of H2O2 treatment on catalase-inactivated peanuts and found that it effectively reduced aflatoxins. Higher treatment temperature and catalase inactivation improved the efficacy of AF reduction. The oil quality of treated peanuts was not significantly affected. The weight loss and oil quality change of the treated peanuts were negligible. Peanuts were able to keep intact after the treatment.
Article
Chemistry, Multidisciplinary
Divyesh S. Shelar, Gauri S. Malankar, Pinky R. Singh, Shashikant P. Vaidya, Rahul V. Pinjari, Sudesh T. Manjare
Summary: The first organoselenium-based quinoline probe for superoxide detection was successfully synthesized and characterized. The probe exhibited high selectivity and sensitivity towards superoxide, with a 64-fold increase in fluorescence intensity. Its detection limit was found to be 1.57 μM. The probe also showed strong anticancer activity in lung carcinoma and breast cancer cells.
NEW JOURNAL OF CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Inna L. Karpenko, Vladimir T. Valuev-Elliston, Olga N. Ivanova, Olga A. Smirnova, Alexander V. Ivanov
Summary: Viral infections lead to increased ROS production, causing oxidative stress and disrupting the antioxidant capacity of cells, while Prdx, the most effective peroxide scavengers, have been largely overlooked. Prdx can scavenge peroxides at physiological concentrations in various cell compartments, showcasing important antioxidant functions that warrant further attention.
Article
Chemistry, Applied
Anna Tama, Grzegorz Bartosz, Izabela Sadowska-Bartosz
Summary: This communication reveals that phenolic compounds commonly found in plant-based food and beverages can interfere with the Ampliflu Red-HRP assay used for quantification of hydrogen peroxide. Concentrations below 10 μM of catechin, propyl gallate, quercetin, and gallic acid were found to decrease the yield of the product, resorufin, by 50% in this system. The extent of inhibition increased with lower hydrogen peroxide concentrations. Therefore, careful interpretation of results is necessary when employing HRP methods for quantifying low concentrations of hydrogen peroxide in materials containing phenolic compounds.
Article
Environmental Sciences
Yixin Huang, Jinbin Lin, Jing Zou, Jiaxin Xu, Mengyun Wang, Huahua Cai, Baoling Yuan, Jun Ma
Summary: Introduction of ABTS as an electron shuttle in the HRP/H2O2 system enhanced the degradation efficiency of diclofenac under neutral pH conditions. The green-colored ABTS radical was identified as the main reactive species in the HRP/H2O2/ABTS system, showing no destruction and serving as an ideal electron shuttle. The reaction conditions including solution pH, HRP concentration, and H2O2 concentration impacted the formation of ABTS radical and the degradation of diclofenac in the system.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Editorial Material
Multidisciplinary Sciences
Ari Zeida, Bruno Manta, Madia Trujillo
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Dayana Benchoam, Jonathan A. Semelak, Ernesto Cuevasanta, Mauricio Mastrogiovanni, Juan S. Grassano, Gerardo Ferrer-Sueta, Ari Zeida, Madia Trujillo, Matias N. Moller, Dario A. Estrin, Beatriz Alvarez
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Review
Pharmacology & Pharmacy
Maria Dolores Pineyro, Diego Arias, Adriana Parodi-Talice, Sergio Guerrero, Carlos Robello
Summary: Chagas Disease, African sleeping sickness, and leishmaniasis are neglected diseases caused by pathogenic trypanosomatid parasites, which have significant impact in poor countries. Current treatment drugs are toxic, difficult to access, and may lead to drug resistance. Inhibiting essential parasite metabolic pathways unique from the host can lead to more efficient and safe drug development.
CURRENT PHARMACEUTICAL DESIGN
(2021)
Article
Biochemistry & Molecular Biology
Lisa K. Folkes, Silvina Bartesaghi, Madia Trujillo, Peter Wardman, Rafael Radi
Summary: Tyrosine plays a critical role in proteins and can undergo oxidative modifications, producing phenoxyl radicals. The reactivity of tyrosine phenoxyl radicals with oxygen and nitric oxide differs significantly, with unclear products from these reactions. The fate of tyrosine phenoxyl radicals in biological systems is determined by the availability of oxidants and reductants.
FREE RADICAL RESEARCH
(2021)
Meeting Abstract
Biochemistry & Molecular Biology
Madia Trujillo
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Infectious Diseases
Luisa Berna, Gonzalo Greif, Sebastian Pita, Paula Faral-Tello, Florencia Diaz-Viraque, Rita De Cassia Moreira De Souza, Gustavo Adolfo Vallejo, Fernando Alvarez-Valin, Carlos Robello
Summary: This study sequenced maxicircles of T. cruzi strains using long-read sequencing technology, revealing a common architecture with four regions. Through phylogenetic analysis and comparison of genomic composition, it was found that T. cruzi is a complex of species composed of various maxicircle variants.
PLOS NEGLECTED TROPICAL DISEASES
(2021)
Review
Biochemistry & Molecular Biology
Lisa K. Folkes, Silvina Bartesaghi, Madia Trujillo, Peter Wardman, Rafael Radi
Summary: The use of free radicals in biology, particularly peroxynitrite-derived radicals, has attracted significant attention. This review discusses the principles and methods of selective radical generation by radiolysis, as well as the key reactions of peroxynitrite-derived radicals with potential biological targets. Additionally, studies involving the reactions of these radicals with lipoic acid/dihydrolipoic acid, hydrogen sulphide, and desferrioxamine are outlined.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Physiology
Lucia Piacenza, Ari Zeida, Madia Trujillo, Rafael Radi
Summary: The free radical nitric oxide (NO) plays a key role in physiological processes, but its bioavailability is compromised in certain disease conditions due to increased production of superoxide radical. This leads to the inactivation of nitric oxide and the formation of peroxynitrite, which is implicated in various human diseases and the aging process. Understanding the complex biochemistry of peroxynitrite is crucial for developing therapeutic interventions.
PHYSIOLOGICAL REVIEWS
(2022)
Article
Biochemistry & Molecular Biology
Maria-Armineh Tossounian, Maria Baczynska, William Dalton, Charlie Newell, Yilin Ma, Sayoni Das, Jonathan Alexis Semelak, Dario Ariel Estrin, Valeriy Filonenko, Madia Trujillo, Sew Yeu Peak-Chew, Mark Skehel, Franca Fraternali, Christine Orengo, Ivan Gout
Summary: Coenzyme A (CoA) plays a crucial role in metabolism and gene regulation. Recent studies have shown that CoA functions as an important antioxidant by forming a disulfide bond with proteins, called CoAlation. This study identified and classified CoAlated proteins from various organisms under stress conditions, and analyzed the stabilization interactions of CoA segments within the modified cysteines. The findings propose three modes of CoA binding to proteins and provide a comprehensive understanding of CoAlation proteins' functional distribution and CoA-protein stabilization interactions.
Review
Biochemistry & Molecular Biology
Ernesto Cuevasanta, Dayana Benchoam, Jonathan A. Semelak, Matias N. Moller, Ari Zeida, Madia Trujillo, Beatriz Alvarez, Dario A. Estrin
Summary: Persulfides are species closely related to thiols and hydrogen sulfide, and can be formed in biological systems. They act as key intermediates in catabolic and biosynthetic processes, and may impact the properties of biomolecules involved.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Giuliana Cardozo, Mauricio Mastrogiovanni, Rafael Radi, Madia Trujillo, Anibal M. Reyes
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Meeting Abstract
Biochemistry & Molecular Biology
Rogerio Aleixo Silva, Renato Domingos, Madia Trujillo, Arnaldo Filho, Cristiano Oliveira, Regina Baldini, Jesmond Dalli, Luis Netto
FREE RADICAL BIOLOGY AND MEDICINE
(2022)
Article
Microbiology
Florencia Diaz-Viraque, Maria Laura Chiribao, Lisvane Paes-Vieira, Matias R. Machado, Paula Faral-Tello, Ramiro Tomasina, Andrea Trochine, Carlos Robello
Summary: Chagas disease, caused by Trypanosoma cruzi, is a widespread infectious disease with no effective treatment. TcAKR, an enzyme involved in the metabolism of the anti-Chagas drugs benznidazole and nifurtimox, was found to have a biological role in the parasite mitochondria and potential involvement in PGF(2)alpha synthesis.
Article
Microbiology
Florencia Diaz-Viraque, Maria Laura Chiribao, Maria Gabriela Libisch, Carlos Robello
Summary: This study revealed the three-dimensional chromatin structure of the Trypanosoma cruzi genome using chromosome conformation capture (Hi-C) technology, showing the formation of C and D chromatin compartments by the core and disruptive regions. These chromatin compartments differ in DNA methylation levels, nucleosome positioning, and chromatin interactions, affecting genome expression dynamics.
NATURE MICROBIOLOGY
(2023)
Article
Microbiology
Maria Dolores Pineyro, Maria Laura Chiribao, Diego G. Arias, Carlos Robello, Adriana Parodi-Talice
Summary: This study investigated the sensitivity and dynamics of Trypanosoma cruzi Prxs to overoxidation in vitro and in an infection context. The results showed that Prxs were overoxidized in parasites treated with oxidants, and the overoxidized forms remained present at least 24 hours after treatment. Overoxidation of Prxs was also observed in amastigotes residing in infected macrophages. Furthermore, the study found that m-TXNPx has a redox-state-dependent chaperone activity.
Article
Biochemistry & Molecular Biology
Yuri Nagaoka, Kotone Oshiro, Yuta Yoshino, Toshiyuki Matsunaga, Satoshi Endo, Akira Ikari
Summary: This study investigated the effect of intercellular adhesion molecule CLDN1 on the anticancer drug sensitivity of small-cell lung cancer (SCLC) cells. It was found that overexpression of CLDN1 decreased the sensitivity of SCLC cells to anticancer drugs and enhanced their migratory capacity through the activation of the TGF-81/EMT signaling pathway. Treatment with EMT inhibitors showed potential in overcoming the reduced sensitivity to anticancer drugs in CLDN1-overexpressing SCLC cells.
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
(2024)
