Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 504, Issue 1, Pages 56-60Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2010.07.024
Keywords
Human macula; Singlet oxygen; Electron paramagnetic resonance (EPR)
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Funding
- National Institute of Health [EY-11600]
- Research to Prevent Blindness, Inc. (New York, NY)
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It is thought that direct quenching of singlet oxygen and scavenging free radicals by macular pigment carotenoids is a major mechanism for their beneficial effects against light-induced oxidative stress. Corresponding data from human tissue remains unavailable, however. In the studies reported here, electron paramagnetic resonance (EPR) spectroscopy was used to measure light-induced singlet oxygen generation in post-mortem human macula and retinal pigment epithelium/choroid (RPE/choroid). Under white-light illumination, production of singlet oxygen was detected in RPE/choroid but not in macular tissue, and we show that exogenously added macular carotenoids can quench RPE/choroid singlet oxygen. When the singlet oxygen quenching ability of the macular carotenoids was investigated in solution, it was shown that a mixture of meso-zeaxanthin, zeaxanthin, and lutein in a ratio of 1:1:1 can quench more singlet oxygen than the individual carotenoids at the same total concentration. (C) 2010 Elsevier Inc. All rights reserved.
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