Journal
ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS
Volume 475, Issue 1, Pages 55-65Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.abb.2008.04.013
Keywords
processivity; mungbean DNA polymerase; family X-DNA polymerase
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Analysis of the processivity of mungbean ddNTP-sensitive DNA polymerase showed the incorporation of similar to 35-40 nucleotides per binding event in the replication assays involving M13 ss DNA template with 5'-labeled 17-mer primer. Optimal processivity was obtained with 100-150 mM KCl and 6-8 mM Mg2+ at pH 7.5. The enzyme showed preference for Mg2+ over Mn2+ as the metal activator for processivity. 2', 3' dideoxythymidine 5' triphosphate (ddTTP) and rat DNA pol beta antibody strongly influenced distributive synthesis. Considerable enhancement in processivity was noticed at 1 mM ATP and 2-4 mM spermidine while higher concentrations of spermidine caused distributive synthesis. The enzyme was found to be active in both meristematic and meiotic tissues and distinctly induced by EMS treatment. DNA-binding assays revealed distinct binding ability of the enzyme to template/primer and damaged DNA substrate. Together these observations illustrate the probable involvement of the enzyme in replication and repair machinery in higher plants. (C) 2008 Elsevier Inc. All rights reserved.
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