Journal
AQUATIC TOXICOLOGY
Volume 100, Issue 4, Pages 373-375Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.aquatox.2010.08.007
Keywords
Verapamil; Sturgeon sperm; Motility; Velocity; Antioxidant response; Protective role
Categories
Funding
- CENAQUA [CZ.1.05/2.1.00/01.0024]
- USB RIFCH [MSM 6007665809]
- Ministry of the Environment of the Czech Republic [SP/2e7/229/07]
- GAJU [047/2010/Z, 003/2010/Z, 007/2010/Z]
- Ministry of Agriculture of People's Republic of China [200903048]
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In the current study, the sperm of Russian sturgeon (Acipenser gueldenstaedtii) was used to evaluate the roles of Verapamil (VRP), a calcium channel blocker, against cadmium (Cd)-induced stress. Sturgeon sperm were exposed for 2 h at 50 mu g/L VRP, 5.0 mu g/L Cd, the mixture of 50 mu g/L VRP + 5.0 mu g/L Cd, 50 mu g/L Cd and the mixture of 50 mu g/L VRP + 50 mu g/L Cd. After exposure, the sperm motility parameters (motility and velocity), oxidative stress levels (lipid peroxidation [LPO] and carbonyl protein [CP]) and antioxidant enzyme activities (superoxide dismutase [SOD], glutathione reductase [GR], glutathione peroxidase [GPx]) were measured in sturgeon sperm. Compared to the control, Cd-induced stress was apparent as reflected by depressed motility parameters, induced oxidative stress and inhibited antioxidant enzyme activities at both Cd concentrations. In the presence of VRP, Cd-induced stress was reduced in sturgeon sperm, especially all the measured parameters in the sperm exposed at 5.0 mu g/L Cd returned to control levels, expect for the sperm motility. The present results indicate that VRP can reduce the Cd-induced stress in sturgeon sperm and suggest that using of sperm in vitro assays may provide a novel and efficient means for evaluating the effects of residual metals in the aquatic environment of sturgeon. (C) 2010 Elsevier B.V. All rights reserved.
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