Article
Multidisciplinary Sciences
Ji-won Choi, Ludvik Alkhoury, Leonardo F. Urbano, Puneet Masson, Matthew VerMilyea, Moshe Kam
Summary: Computer-Assisted Semen Analysis (CASA) is a reliable technique for analyzing semen images, and it can process a large number of images with high consistency, accuracy, and repeatability. Simulation models of semen images enable objective assessments of CASA algorithms and can be used to study algorithms for segmentation, localization, and tracking of sperm cells.
SCIENTIFIC REPORTS
(2022)
Article
Obstetrics & Gynecology
Elisabetta Baldi, Meurig T. Gallagher, Stepan Krasnyak, Jackson Kirkman-Brown
Summary: The sixth edition of the World Health Organization manual explores extended examination methods that offer key diagnostics for investigating male reproductive system function. These methods go beyond basic semen analysis and can specifically guide the clinical characterization of fertile or infertile men. Multiparametric scoring for sperm morphological defects, sperm DNA fragmentation, and computer-assisted analysis of sperm or semen are among the most widely used and debated techniques.
FERTILITY AND STERILITY
(2022)
Article
Developmental Biology
Brett Nixon, Amanda L. Anderson, Elizabeth G. Bromfield, Jacinta H. Martin, Shenae L. Cafe, David A. Skerrett-Byrne, Matthew D. Dun, Andrew L. Eamens, Geoffry N. De Iuliis, Stephen D. Johnston
Summary: Research focused on the physiology of saltwater crocodile sperm, showing an increasing gradient of sperm motility as they transit the male reproductive tract, and that increasing intracellular cAMP levels significantly enhance sperm motility. This study paves the way for developing artificial reproductive technologies and opportunistic recovery, storage, and potential utilization of post-mortem spermatozoa from genetically valuable animals.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Article
Veterinary Sciences
Josue Calderon-Calderon, Francisco Sevilla, Eduardo R. S. Roldan, Vinicio Barquero, Anthony Valverde
Summary: This study aimed to evaluate the effect of fat-soluble vitamin supplementation on the seminal quality of boars. The results showed that fat-soluble vitamin supplementation increased semen volume, motility, progressive motility, and velocity variables. Overall, dietary fat-soluble vitamin supplementation significantly improved the semen quality of boar ejaculates.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Rossella Cannarella, Francesca Mancuso, Nunziata Barone, Iva Arato, Cinzia Lilli, Catia Bellucci, Marco Musmeci, Giovanni Luca, Sandro La Vignera, Rosita A. Condorelli, Aldo E. Calogero
Summary: This study evaluated the expression of the follicle-stimulating hormone receptor (FSHR) in human spermatozoa and its effects on sperm function. The results showed that FSHR is expressed in the post-acrosomal segment, neck, midpiece, and tail of human spermatozoa, with no difference in localization between patients and controls. Incubation with FSH increased FSHR expression in patients. Furthermore, FSH incubation had a negative effect on sperm motility and mitochondrial function in both patients and controls, but more severely in controls. FSH also activated the AKT(473) signaling pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Fisheries
Alexandre Erraud, Marc Bonnard, Valerie Cornet, Imen Ben Ammar, Sascha Antipine, Quentin Peignot, Jerome Lambert, Syaghalirwa N. M. Mandiki, Patrick Kestemont
Summary: By investigating the impact of different biological factors on sperm quality and reproductive success, it was found that sperm quality of early parr from captivity was poorer when subjected to cryopreservation, resulting in reduced reproductive success. Therefore, it is recommended that only the semen of anadromous males should be cryopreserved.
Article
Fisheries
S. Judycka, D. Zarski, M. A. Dietrich, H. Karol, P. Hliwa, M. Blazejewski, A. Ciereszko
Summary: The study focuses on an improved semen cryopreservation protocol for commercial fertilization in European perch, showing high sperm motility with a new extender and KCl supplementation. The enhanced procedure allows for handling prediluted semen and maintaining high fertilization rates for cryopreserved and fresh semen, enabling large-scale fertilization applications.
Article
Fisheries
Daniella Aparecida de Jesus Paula, Naiara Melo, William Franco Carneiro, Pedro Leite de Castro Uzeda, Bruna Resende Chaves, Isaac Filipe Moreira Konig, Luis David Solis Murgas
Summary: This study aimed to develop an alternative protocol for freezing fish semen using an ultra-freezer. The results demonstrate that this method is efficient in maintaining semen quality.
AQUACULTURE INTERNATIONAL
(2022)
Article
Fisheries
Takafumi Fujimoto, Takahisa Kaneyasu, Mitsuru Endoh, Yuya Kogame, Joanna Nynca, Andrzej Ciereszko, Eisuke Takahashi, Etsuro Yamaha, Kiyoshi Naruse, Katsutoshi Arai
Summary: The aim of this study was to evaluate the cryopreservation of masu salmon semen using a specific extender and to investigate the relationship between semen parameters and post-thaw sperm motility. The results showed that the suitability of semen for cryopreservation was affected by the condition of the parental fish.
Article
Fisheries
Sylwia Judycka, Mariola A. Dietrich, Daniel Zarski, Halina Karol, Katarzyna Palinska-Zarska, Maciej Blazejewski, Andrzej Ciereszko
Summary: This study aimed to investigate the effects of final glucose and sperm concentrations, different activation solutions for sperm motility, and dilution of semen on the cryopreservation success of cultured pikeperch semen. Results showed that specific concentrations and dilution methods had significant impacts on sperm motility and successful cryopreservation. Future efforts should focus on controlling spontaneous motility to further improve cryopreservation techniques for pikeperch semen.
Article
Agriculture, Dairy & Animal Science
Beata Irena Cejko, Slawomir Krejszeff, Agata Anna Cejko, Katarzyna Dryl
Summary: In this study, the researchers compared the effectiveness of four activation solutions for ide sperm motility analysis. They found that adding a minimum concentration of bovine serum albumin (BSA) and casein significantly improved the computer-assisted sperm analysis (CASA) parameters of ide sperm. These low doses of proteins were also effective in preventing sperm adhesion to glass slides.
Article
Cell Biology
Joao C. Ribeiro, Raquel L. L. Bernardino, Ana Goncalves, Alberto Barros, Giuseppe Calamita, Marco G. G. Alves, Pedro F. F. Oliveira
Summary: Osmoregulation is essential for sperm function, and aquaglyceroporins play a crucial role in glycerol permeability, which is important for sperm motility. AQP7 is especially significant in glycerol transport, and its impairment may lead to reduced sperm motility in asthenozoospermic individuals.
Article
Biochemistry & Molecular Biology
Tejaswi Yarra, Mark Blaxter, Melody S. Clark
Summary: This study identified proteins likely involved in shell calcification by analyzing gene expression during repair in bivalve molluscs, expanding the number of candidate biomineralization proteins. The research also defines a minimal functional protein domain set required to produce solid microstructures from soluble calcium carbonate, with implications for molluscan shell evolution, environmental impacts on biomineralization, materials science, and biomimicry research.
MOLECULAR BIOLOGY AND EVOLUTION
(2021)
Article
Developmental Biology
Md Mazharul Islam, Takashi Umehara, Natsumi Tsujita, Masanori Koyago, Masayuki Shimada
Summary: This study demonstrates that exogenous cholesterol treatment can enhance the quality of frozen-thawed bull sperm by restoring the cholesterol balance in sperm plasma and organelle membranes. The addition of exogenous cholesterol protects the plasma membrane and maintains mitochondrial function, thereby improving sperm motility and fertility. Therefore, the application of cholesterol after thawing is a promising option for improving the fertility of frozen-thawed sperm.
MOLECULAR HUMAN REPRODUCTION
(2023)
Article
Agriculture, Dairy & Animal Science
Pfunzo T. Muvhali, Maud Bonato, Irek A. Malecki, Schalk W. P. Cloete
Summary: This study evaluated the relationship between computer-aided sperm analysis (CASA) technology and mass sperm motility scoring in ostriches. Positive correlations were found between these methods for assessing sperm motility traits, suggesting that the affordable mass sperm motility scoring can be used by farmers to screen ostrich males for sperm quality.
Article
Fisheries
Y. P. Song, M. Suquet, I. Queau, L. Lebrun
Article
Fisheries
Marc Suquet, Ricardo Gonzales Araya, Luc Lebrun, Isabelle Queau, Christian Mingant, Rene Robert
Article
Fisheries
Ricardo Gonzalez-Araya, Luc Lebrun, Claudie Quere, Rene Robert
Article
Fisheries
M. Suquet, F. Rimond, J. Cosson, J. Wilson-Leedy, L. Lebrun, I. Queau, C. Mingant, C. Fauvel
JOURNAL OF APPLIED ICHTHYOLOGY
(2013)
Article
Environmental Sciences
Marion Desdouits, Jean-Come Piquet, Candice Wacrenier, Cecile Le Mennec, Sylvain Parnaudeau, Sarah Jousse, Sophie Rocq, Lionel Bigault, Maud Contrant, Pascal Garry, Fabienne Chavanon, Raoul Gabellec, Laure Lamort, Luc Lebrun, Patrik Le Gall, Claire Meteigner, Anne Schmitt, Jean Luc Seugnet, Ophelie Serais, Cecile Peltier, Celine Bressolette-Bodin, Yannick Blanchard, Francoise S. Le Guyader
Summary: The study aimed to assess methods for detecting CoV in the coastal environment, with results showing low yields for PEDV using seawater-based methods, the current norm for norovirus detection applicable to CoV in shellfish, and no contamination of French shores by SARS-CoV-2.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Environmental Sciences
Isabelle Amouroux, Jean-Louis Gonzalez, Stephane Guesdon, Maria Jesus Belzunce-Segarra, Philippe Bersuder, Thi Bolam, Miguel Caetano, Margarida Correia Dos Santos, Joana Larreta, Luc Lebrun, Barbara Marras, Vanessa Millan Gabet, Brendan McHugh, Iratxe Menchaca, Florence Menet-Nedelec, Natalia Montero, Olivier Perceval, Olivier Pierre-Duplessix, Fiona Regan, Jose German Rodriguez, Marta Rodrigo Sanz, Marco Schintu, Blanaid White, Hao Zhang
Summary: Integrative passive samplers, such as DGT, have the potential to improve the assessment quality. However, adaptation of AA-EQS for DGTs should be pursued before using DGT results in a regulatory context. This study proposes a methodology for using DGT results in the chemical status assessment of marine waters.
ENVIRONMENTAL SCIENCES EUROPE
(2023)
Article
Fisheries
M. Suquet, C. Amourda, C. Mingant, I. Queau, L. Lebrun, R. Brizard
Article
Fisheries
J Aubin, FJ Gatesoupe, L Labbé, L Lebrun
AQUACULTURE RESEARCH
(2005)