Article
Biotechnology & Applied Microbiology
Susan Wagner, Sarah L. Whiteley, Meghan Castelli, Hardip R. Patel, Ira W. Deveson, James Blackburn, Clare E. Holleley, Jennifer Marshall A. Graves, Arthur Georges
Summary: This study conducted a comprehensive transcriptome-wide analysis of sex differentiation in central bearded dragon embryos. It was found that dmrt1 and amh play a key role in the male pathway, whereas sox9 is not differentially expressed at the bipotential stage. Interestingly, the expression levels of the male pathway genes amh and sox9 are high in female gonads. It is proposed that the interpretation of GSD mechanisms in reptiles should not solely rely on mammalian experience.
Review
Biology
Preetha Rajendiran, Faizul Jaafar, Sonika Kar, Chenichery Sudhakumari, Balasubramanian Senthilkumaran, Ishwar S. Parhar
Summary: The sex determination and differentiation in teleosts are influenced by genetics, environmental factors, and brain function, which are species-specific processes involving various genes and environmental factors. The role of the brain in sex determination and differentiation of teleost is significant but still unclear.
Article
Agriculture, Dairy & Animal Science
Liping Shi, Feibiao Song, Kaixi Zhang, Yue Gu, Jinghan Hu, Junlong Sun, Zhongwei Wang, Li Zhou, Jian Luo
Summary: We performed transcriptome sequencing and analysis to identify sex-biased miRNAs in T. blochii. Our study revealed that these miRNAs are associated with sex differentiation and gonadal development signaling pathways. Co-expression network analysis identified several miRNAs that may play pivotal roles in gonadal development of T. blochii. This research provides new insights into the function of miRNAs in sex differentiation and determination in T. blochii.
Article
Agriculture, Dairy & Animal Science
Huapu Chen, Zhiyuan Li, Yaorong Wang, Hai Huang, Xuewei Yang, Shuangfei Li, Wei Yang, Guangli Li
Summary: Spot-fin porcupine fish (Diodon hystrix) has been identified as a new and economically valuable aquaculture species in south China but the lack of understanding of reproductive regulation is a major barrier for its commercial aquaculture. This study analyzed the gonadal transcriptomes of D. hystrix, identifying genes associated with gonadal development and gametogenesis, providing a useful resource for further research on reproductive manipulation in this species. The comparison of ovary and testis transcriptomes revealed differentially expressed genes and highlighted the need for more genetic information to advance the management of breeding and reproduction in D. hystrix.
Article
Endocrinology & Metabolism
Yunqi Jiang, Zhelun Peng, Qiu Man, Sheng Wang, Xiaochen Huang, Lu Meng, Heng Wang, Guiyu Zhu
Summary: Birds exhibit left/right asymmetry in gonad development, with the left gonad developing into a functional ovary in females while the right gonad regresses. This asymmetry is not present in males. The study explores the role of H3K27ac marked chromatin activation in avian gonad development and reveals that the discrepancy in H3K27ac distribution contributes to asymmetric gonadal development only in females. Other mechanisms are responsible for the symmetric development of male gonads.
BIOLOGY OF SEX DIFFERENCES
(2022)
Article
Cell Biology
Martin A. Estermann, Mylene M. Mariette, Julie L. M. Moreau, Alexander N. Combes, Craig A. Smith
Summary: The differentiation of gonadal supporting cells in birds is conserved among different clades, with PAX2 expression down-regulation defining the onset of gonadal sex differentiation in each species. Further comparative analysis in avian species is necessary to fully understand the mechanisms of avian sex determination and gonadal differentiation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Zhongkai Cui, Jie Wang, Yingming Yang, Zhangfan Chen, Qian Wang, Jialin Wang, Tingting Zhang, Wenteng Xu, Songlin Chen
Summary: This study reveals the role of the cyp26b1 gene in sex differentiation and gonadal development of the Chinese tongue sole, providing important insights for the research on fish sex differentiation.
Article
Fisheries
Meng Chen, Yingping Zhang, Meidong Zhang, Chunjuan Qu, Guiwei Zou, Hongwei Liang
Summary: In this study, the role of Wnt5b in sex differentiation in Pelodiscus sinensis was investigated. The expression of Wnt5b in diverse tissues and embryonic gonads was analyzed using qRT-PCR. The results showed that Wnt5b was highly expressed in embryonic gonads at early stages and had higher expression in female gonads than in male gonads. Wnt agonists significantly affected the expression of Wnt5b in male and female embryonic gonads.
AQUACULTURE RESEARCH
(2022)
Article
Environmental Sciences
Hanhan Yao, Zhihua Lin, Yinghui Dong, Xianghui Kong, Lin He, Liangyi Xue
Summary: This study investigated potential sex-related genes in the adult gonads of the razor clam through comparative transcriptome analysis, identifying genes involved in sex determination/differentiation and gametogenesis. Validation of gene expression profiles by qRT-PCR confirmed the reliability and accuracy of the RNA-Seq results.
FRONTIERS IN MARINE SCIENCE
(2021)
Article
Fisheries
Xi Wu, Yang Yang, Tong Wang, Chaoyue Zhong, Yuhao Tao, Leyi Chang, Zining Meng, Xiaochun Liu
Summary: In this study, transcriptome sequencing of brown-marbled grouper at 117 dph and 259 dph was performed to investigate the molecular mechanism of sex differentiation. The results showed that genes in the retinoic acid signal pathway are involved in the early stage of sex differentiation. Histological observations revealed the classification of germ cells in the 259 dph gonad into six stages. Additionally, male-related genes such as gsdf, sox9, dmrt1, and dmrt3 may play roles in sex differentiation. This study provides valuable information for understanding the molecular mechanism of sex differentiation in fish.
AQUACULTURE REPORTS
(2023)
Article
Biotechnology & Applied Microbiology
Sarah L. Whiteley, Clare E. Holleley, Arthur Georges
Summary: The study found that the ZZ genotype has a stronger influence on sexual fate than temperature, with any exposure to low temperatures leading to testes differentiation, while sex reversal required longer exposure to high temperatures. Ovotestes were shown to exist along a spectrum of femaleness to maleness at the transcriptional level. Inter-individual variation in gene expression changes following temperature switches was found, suggesting that genetic sensitivity to temperature cues and the timing and duration of the cue influence sex reversal.
Article
Cell Biology
Xin Wang, Fengling Lai, Dantong Shang, Yibin Cheng, Tian Lan, Hanhua Cheng, Rongjia Zhou
Summary: The study provides a comprehensive single-cell developmental atlas of ovotestis and reveals the interactions between germline and niche cells that determine the fate of male and female germlines. The findings offer novel insights into ovotestis development and shed light on the cellular fate map shaping cell differentiation in ovotestis.
CELL DEATH & DISEASE
(2021)
Article
Fisheries
Zi-Wei Chen, Xing-Kun Jin, Fan-Xiang Gao, Jian-Fang Gui, Zhe Zhao, Yan Shi
Summary: The structure and function of gut microbiota in fish can be influenced by fish sex, but the impact is limited, mainly affecting certain bacterial taxa. The relative abundance of sex-specific bacterial taxa plays a role in determining bacterial phenotypes.
Article
Fisheries
Irfan A. Bhat, Jaffer Y. Dar, Irshad Ahmad, Ishfaq N. Mir, Hussna Bhat, Raja A. H. Bhat, Parvaiz A. Ganie, Rupam Sharma
Summary: The normal ontogenesis of the gonads is crucial for successful reproduction in fish, as in higher animals. The genetic cascade plays a key role in gonadal differentiation and development. Hormones of the hypothalamic-pituitary-gonadal axis regulate reproduction and fertility by influencing the production and function of gonadal sex steroids.
REVIEWS IN AQUACULTURE
(2021)
Article
Fisheries
M. Ramon, M. J. Amor, E. Galimany
Summary: This study aims to characterize the reproductive ecology of Parastichopus regalis in the northwestern Mediterranean Sea, including its sex ratio, gonad description, and reproductive cycle. The results show that P. regalis has an annual reproductive cycle, maturing during spring and spawning in summer. The description of these reproductive characteristics fills an important information gap for future management of the commercial fishery, which is currently unregulated.
FISHERIES RESEARCH
(2022)