Article
Fisheries
Cahya Fusianto, Paul M. Hick, Murwantoko, Afri Herlambang, Richard J. Whittington, Joy A. Becker
Summary: A diagnostic study was conducted on mass mortality events in hybrid groupers in sea cages off the northern coast of Bali in 2016. The study revealed a high prevalence of NNV infection, with no significant differences between fish with and without clinical signs. MCV infection was also prevalent, with a significant proportion of clinically affected fish showing pathology consistent with ISKNV infection.
AQUACULTURE REPORTS
(2021)
Article
Fisheries
Youhua Huang, Liwei Huang, Ya Zhang, Qi Zheng, Liwen Xu, Qiwei Qin, Xiaohong Huang
Summary: We established a new cell line from sea perch fry, which predominantly consisted of epithelial-like cells and proliferated well under specific culture conditions. These SPF cells showed susceptibility to different viruses and underwent non-apoptotic cell death upon infection. Additionally, interferon-related genes were up-regulated in virus-infected SPF cells, indicating the involvement of host interferon immune response in virus infection.
Article
Biology
Joan Tang Xiao Joe, Yung-Che Tseng, Jen-Leih Wu, Ming-Wei Lu
Summary: The intestinal microbiota communities in grouper infected with pathogens show decreased microbial diversity and increased microbial richness. Pathogen-infected grouper have higher levels of harmful genera in their intestines, while beneficial genera predominate in the intestines of the control group. In addition, immune-related genes such as immunoglobulin T and proinflammatory cytokines show increased expression levels in the intestine after pathogen infection.
Article
Fisheries
Min Yang, Qing Wang, Jinpeng Chen, Yuxin Wang, Yong Zhang, Qiwei Qin
Summary: The red-spotted grouper, a commercially important species in China and Southeast Asia, faces a serious threat from RGNNV disease outbreaks. By conducting a GWAS, researchers identified candidate genes involved in nervous system development, retinal formation, and lipid metabolism regulation, suggesting potential pathways to enhance disease resistance. This study provides insights for marker-assisted selection in breeding programs aiming to improve red-spotted grouper's resilience to RGNNV.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Sumit Vinod Jungi, Pakkakul Sangsuriya, Suwimon Taengphu, Kornsunee Phiwsaiya, Molruedee Sonthi, Bunlung Nuangsaeng, Krishna R. Salin, Saengchan Senapin, Ha Thanh Dong
Summary: This study identified Nervous Necrosis Virus (NNV) of the RGNNV genotype as a virus associated with massive die-offs in imported pearl grouper fry in Thailand. The virus was found to localize in the vacuolation lesions of the central nervous system and retina of infected fish. Appropriate biosecurity measures are needed to prevent any loss to the marine aquaculture industry considering the infectious nature and broad host range of the virus.
Article
Immunology
Tianwen Lin, Jing Xing, Xiaoqian Tang, Xiuzhen Sheng, Heng Chi, Wenbin Zhan
Summary: In this study, a bicistronic DNA vaccine targeting the interferon regulatory factor 3 (IRF3) of pearl gentian grouper was successfully constructed. The vaccine expressed both capsid protein (CP) and IRF3 and elicited cellular, humoral, and local immune responses in the grouper, providing effective protection against NNV infection.
Article
Fisheries
Linna Wang, Yongsheng Tian, Zhentong Li, Ziqi Li, Shuai Chen, Linlin Li, Wensheng Li, Qingbin Wang, Haowei Lin, Bo Li
Summary: The study characterized the gut microbiota of yunlong grouper and found differences in bacterial composition between asymptomatic and diseased fish groups. This provides insight into the interaction between gut microbiota and host under NNV infection.
AQUACULTURE RESEARCH
(2022)
Article
Virology
Si-Woo Kim, Soo-Jin Kim, Myung-Joo Oh
Summary: Immunization at low temperature with NNV in sevenband grouper resulted in increased resistance against NNV infection, leading to lower mortality rates and decreased disease symptoms.
Article
Fisheries
Zhaodi Sun, Yuqin Ren, Yitong Zhang, Yucong Yang, Guixing Wang, Zhongwei He, Yufeng Liu, Wei Cao, Yufen Wang, Yuanshuai Fu, Jilun Hou
Summary: Fish cell lines serve as important tools in biological studies. Researchers have established a new Japanese flounder brain (JFB) cell line, confirming its origin and characteristics, which can be used for studying gene manipulation, host-virus interaction, and vaccine development.
Article
Fisheries
Menglan Zhang, Zhijie Lu, MeiZhen Tang, Gan Pan, Lijuan Zhao, Zhendong Qin, Li Lin
Summary: The study found that Epinephelus coioides mannose receptor (EcMR) responds to virus infection by modulating virus-induced apoptosis and inflammation.
Article
Forestry
Lu-Ke Zhang, Ke-Cheng Zhu, Hua-Yang Guo, Bao-Suo Liu, Bo Liu, Nan Zhang, Dian-Chang Zhang
Summary: A new cell line derived from the muscle tissue of the golden pompano, called golden pompano muscle (GPM), has been successfully developed and characterized in this study. GPM cells exhibit fibroblast-like morphology and have a high proliferation rate in Leibovitz's L-15 medium supplemented with fetal bovine serum. The GPM cell line has been stable for over 80 generations and can be cryopreserved. It has been confirmed that the GPM cells used in this study are from the golden pompano based on molecular characterization and chromosome analysis. In addition, GPM cells are susceptible to red-spotted grouper nervous necrosis virus (RGNNV) and can be used for gene expression studies and viral pathogenesis research. The interaction between host and virus can also be investigated using this cell line.
Article
Fisheries
A. Toffan, L. Biasini, T. Pretto, M. Abbadi, A. Buratin, R. Franch, G. Dalla Rovere, V. M. Panzarin, A. Marsella, L. Bargelloni, F. Pascoli
Summary: The study revealed that gilthead sea bream larvae are susceptible to RGNNV/SJNNV infection regardless of age, with clear clinical signs and mortality observed only in the youngest group. Survivors tested negative for specific antibodies. The replication efficiency of RGNNV/SJNNV was higher in 21-day-old larvae, correlating with disease outcomes.
Article
Immunology
Alberto Falco, Melissa Bello-Perez, Rocio Diaz-Puertas, Matthew Mold, Mikolaj Adamek
Summary: Different inactivation procedures were tested for developing an inactivated vaccine against a new deadly NNV isolate affecting European seabass, with UV-C irradiation and formalin treatment showing promising results as potential NNV-inactivation procedures for vaccine candidates.
Article
Fisheries
Wengang Xu, Kiyoshi Soyano, Huafeng Zou, Masaki Nagae
Summary: This study investigated the expression of follicle-stimulating hormone and luteinizing hormone and related genes in the red-spotted grouper during different stages of ovarian differentiation and development. The results showed that the expression levels and distribution of these hormones varied at different developmental stages, while the expression levels of kiss, gpr54, and gnrh1 genes remained relatively stable.
AQUACULTURE REPORTS
(2022)
Article
Fisheries
Rahul Krishnan, Jong-Oh Kim, Yo-Seb Jang, Myung-Joo Oh
Summary: PSMB8 serves as an important regulator of NF-xB signaling during nervous necrosis virus (NNV) infection in sevenband grouper, showing the ability to reduce virus-induced activation of NF-xB promoters and downregulate the expression of proinflammatory cytokines and interferons.
FISH & SHELLFISH IMMUNOLOGY
(2021)
Article
Fisheries
Hunter S. Bailey, Ashley N. Fincannon, Lee A. Fuiman
Summary: This study investigated the transfer of fatty acids from broodstock diets to eggs and the time needed for the eggs to equilibrate to the diet in Southern flounder. The findings indicate that different diet treatments significantly affect the fatty acid composition of eggs, and it takes 8-16 weeks for the eggs to adapt to changes in the adult diet. These results are important for improving broodstock diets and feeding protocols in stock-enhancement programs.
Article
Fisheries
Yu Liu, Mingtao Lei, Hector Victor, Yan Wang
Summary: This study confirms the feasibility of replacing raw fish diet with a formulated diet in commercial farming of large yellow croaker, which can increase fish survival and yield, and reduce feed cost and body lipid deposition.
Article
Fisheries
Xiaoyan Zhang, Lize San, Yucong Yang, Yuehong Tao, Jiangong Ren, Yufeng Liu, Zhongwei He, Jiashuo Di, Ziteng Pei, Guixing Wang, Jilun Hou
Summary: Gynogenesis is an effective technique for establishing homogeneous lines and confirming potential chromosomal mechanisms of sex determination in fish. This study successfully induced gynogenetic families in Thamnaconus modestus and established a complete method for gynogenesis induction.
Article
Fisheries
Julieta C. Martinelli, Megan Considine, Helen R. Casendino, Carolyn M. Tarpey, Isadora Jimenez-Hidalgo, Jacqueline L. Padilla-Gamino, Teri L. King, Lorenz Hauser, Steven Rumrill, Chelsea L. Wood
Summary: Shell-boring polychaetes have caused significant damage to mariculture operations worldwide, particularly in the US Pacific region. This study provides a comprehensive dataset on the prevalence, species distribution, and environmental associations of these parasites, highlighting the impact of season, culturing methods, and environmental factors on infestation.
Article
Fisheries
Juan Gao, Xueshan Li, Kangle Lu, Kai Song, Jian Zhang, Ling Wang, Chunxiao Zhang
Summary: This study investigated the effects of dietary protein levels on growth performance, antioxidant capacity, digestive enzyme activity, and metamorphosis rate of bullfrog tadpoles. The optimal dietary protein level for bullfrog tadpoles was estimated to be 42.49%-46.50% of the diet.
Article
Fisheries
Qiang Li, Lijun Duan, Dongsheng Jin, Yuxin Chen, Yirong Lou, Qianjin Zhou, Zhongjie Xu, Fangjie Chen, Hongxian Chen, Guizong Xu, Maocang Yan, Guanjun Yang, Jianfei Lu, Yanjun Zhang, Jiong Chen
Summary: This study developed a centrifugal microfluidic chip with on-chip RPA to detect five pathogenic microorganisms. The chip enabled the parallel analysis of six genetic markers from a single sample and allowed for the highly automated detection of multiple samples. Compared with PCR and DNA sequencing, the on-chip RPA assay showed high sensitivity and specificity for detecting multiple pathogens in shrimp aquaculture.
Article
Fisheries
Xingchen Huo, Pengxu Wang, Fengxia Zhao, Qian Liu, Qingqing Tian, Lingjie Tang, Maolin Lv, Zhaohui Wei, Chunrong Yang, Jianguo Su
Summary: Bacterial diseases in aquaculture ponds have negative effects on sustainable development. The use of composite I20 biotherapy has been shown to effectively treat bacterial diseases in bullfrog ponds, providing a new strategy for controlling bacterial diseases in aquaculture.
Article
Fisheries
Peng Yin, Takaya Saito, Per Gunnar Fjelldal, Bjorn Tharandur Bjornsson, Sofie Charlotte Remo, Sandeep Sharma, Rolf Erik Olsen, Kristin Hamre
Summary: This study aims to investigate the effect of seasonal changes in temperature and photoperiod on the redox metabolism of Atlantic salmon at the molecular level. The research findings suggest that the changing photoperiod and temperature influenced the redox metabolism of Atlantic salmon, highlighting the importance of oxidative status in the ecological implications.
Article
Fisheries
Nicholas Oppong Mensah, Jeffery Kofi Asare, Emmanuel Tetteh-Doku Mensah, Ernest Christlieb Amrago, Frank Osei Tutu, Anthony Donkor
Summary: This study investigates aquaculture farmers' preference for climate-smart aquaculture insurance products, the challenges they face, and their preferred insurance coverage. The results show that farmers prefer Climate-Induced Aquaculture Stock Mortality Insurance and the most significant constraint is the delay in claim settlement.
Article
Fisheries
Qiu-Ping Chai, Pei Wu, Wei-Dan Jiang, Yang Liu, Hong-Mei Ren, Xiao-Wan Jin, Lin Feng, Xiao-Qiu Zhou
Summary: The study found that appropriate levels of potassium diformate (KDF) can enhance the immune defense and mediate the inflammatory process in fish, possibly through the regulation of T cell differentiation via JAK-STAT and NF-kappa B signaling pathways.
Article
Fisheries
Tian Zhu, Haomin Jia, Haopeng Zhang, Yujing Xiao, Cui Han, Jiaxin Yang
Summary: Chicken manure has significant effects on the cultivation of Chlorella and rotifers. The optimal amount of chicken manure extract for continuous cultivation is 10 mLL-1. Chicken manure can promote the growth of Chlorella and improve the fatty acid composition, indirectly enhancing the growth condition and fatty acid content of rotifers.
Article
Fisheries
Zheng Luo, Yang Yu, Zhenning Bao, Fuhua Li
Summary: This study analyzed the heritability and genetic correlation of two growth traits in Pacific white shrimp and evaluated the genomic prediction using different genomic selection models. The results showed that the NeuralNet model had the highest prediction accuracy and better prospects for predicting shrimp growth traits.
Article
Fisheries
Alberto Ruiz, Ignasi Sanahuja, Karl B. Andree, Dolors Furones, Paul G. Holhorea, Josep A. Calduch-Giner, Jose J. Pastor, Marc Vinas, Jaume Perez-Sanchez, Sofia Morais, Enric Gisbert
Summary: Supplementing diets with a combination of spice oleoresins can reduce lipid accumulation and inflammation in farmed fish. The combination of spice oleoresins also has an immunomodulatory effect on the fish's intestinal immune system, potentially offering anti-inflammatory benefits.
Article
Fisheries
Beibei Zhao, Renhui Mei, Le Li, Di Hu, Lei Li
Summary: This study reveals that JfCXCL8_L1a and JfCXCL8_L1b have different immune pathways, and JfCXCL8_L1b plays a significant role in enhancing the adaptive immunity of T cell-dependent antigen.
Article
Fisheries
Yuhang Liu, Danying Cao, Nan Wu, Xuyang Zhao, Qingsong Zhu, Lian Su, Fatima Altaf, Qianqian Zhang, Haokun Liu, Yongming Li, Bruno Hamish Unger, Yingyin Cheng, Wanting Zhang, Aihua Li, Yaping Wang, Xiao-Qin Xia
Summary: Based on previous research, sinomenine has been found to protect mucosal immunity in farmed fish species by preventing intestinal pathological changes and regulating gene expression related to inflammation. It also enhances immune homeostasis and controls the growth of pathogenic bacteria.