Journal
APPLIED SURFACE SCIENCE
Volume 258, Issue 7, Pages 2783-2787Publisher
ELSEVIER
DOI: 10.1016/j.apsusc.2011.10.132
Keywords
Magnetite; Magnetite-gold; Thiol modification; Enzyme immobilization
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Funding
- Polish National fund [N N204246435]
- EU [POPW.01.03.00-20-034/09-00]
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In the present study Fe3O4 magnetic nanoparticles were synthesized by coprecipitation of Fe2+ and Fe3+ from chlorides. In the next step magnetite-gold core-shell nanoparticles were synthesized from HAuCl4 using an ethanol as a reducing agent. Finally, magnetic nanoparticles were functionalized by hexadecanethiol. The immobilization of biological molecules (trypsin and glucose oxidase) to the thiol-modified and unmodified magnetite-gold nanoparticles surface was tested. The resulting nanoparticles were characterized by infrared spectroscopy, differential scanning calorimetry, Mossbauer spectroscopy and transmission electron microscopy. (C) 2011 Elsevier B.V. All rights reserved.
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