4.6 Article

Spectroscopic Diagnosis of Myocardial Infarction and Heart Failure by Fourier Transform Infrared Spectroscopy in Serum Samples

Journal

APPLIED SPECTROSCOPY
Volume 64, Issue 3, Pages 262-267

Publisher

SAGE PUBLICATIONS INC
DOI: 10.1366/000370210790918508

Keywords

Artificial neural networks; Cluster analysis; Transflection; Transmission; Fourier transform infrared spectroscopy; FT-IR spectroscopy; Heart failure; Myocardial infarction

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Cardiovascular disease is the leading cause of death in Western civilization. In this pilot study we evaluated a new method for the diagnosis of myocardial infarction and heart failure by determining the typical fingerprint in the infrared (IR) spectrum of 1 mu L of a dried patient serum sample by Fourier transform IR spectroscopy. For classification, cluster analysis and artificial neural networks (ANN) were applied. In this study 567 subjects were enrolled, comprising 225 controls (Co) and 342 patients with myocardial infarction (MI) (n = 157) and heart failure (HF) (n = 185). By applying artificial neural network algorithms, the following sensitivities and specificities of the same spectra were determined: MI versus Co (98%, 97%), HF versus Co (98%, 100%), MI versus HF (100%, 100%), and MI plus HF versus Co (100%, 100%). Based on our data, mid-IR spectroscopy appears to be a promising new method to diagnose heart diseases from serum samples. Artificial neural network algorithms proved to be superior to cluster analysis for correct prediction.

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