Journal
APPLIED PHYSICS LETTERS
Volume 104, Issue 20, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.4879457
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Funding
- Australian Research Council [DE120101402]
- Australian Research Council [DE120101402] Funding Source: Australian Research Council
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Here, we demonstrate the unique features of a hydrodynamic based microchip for creating continuous chains of model yeast cells. The system consists of a disk shaped microfluidic structure, containing narrow orifices that connect the main channel to an array of spoke channels. Negative pressure provided by a syringe pump draws fluid from the main channel through the narrow orifices. After cleaning process, a thin layer of water is left between the glass substrate and the polydimethylsiloxane microchip, enabling leakage beneath the channel walls. A mechanical clamp is used to adjust the operation of the microchip. Relaxing the clamp allows leakage of liquid beneath the walls in a controllable fashion, leading to formation of a long cell chain evenly distributed along the channel wall. The unique features of the microchip are demonstrated by creating long chains of yeast cells and model 15 mu m polystyrene particles along the side wall and analysing the hydrogen peroxide induced death of patterned cells. (C) 2014 AIP Publishing LLC.
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