Journal
APPLIED PHYSICS LETTERS
Volume 98, Issue 21, Pages -Publisher
AMER INST PHYSICS
DOI: 10.1063/1.3595482
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Funding
- Army Research Office [W911NF-08-1-0431]
- Multi-University Research Initiative (MURI) from the Department of Defense
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We developed a rapid multifoci-scan confocal Raman microscopy system for label-free molecular imaging of single living cells. A pair of galvo-mirrors were used to raster scan a single laser to generate multifoci excitations and another galvo-mirror synchronously projected Raman scattering from each foci onto a multichannel spectrograph such that multiple spectra were collected simultaneously. The image acquisition time is similar to 40 times faster than in conventional point-scan Raman microscopy with diffraction-limited resolution retained. We demonstrated that this system can be used to monitor the germination dynamics of single bacterial spores with about 1.0 min resolution and 2.5 mW power at each focal point. (C) 2011 American Institute of Physics. [doi: 10.1063/1.3595482]
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