Journal
APPLIED BIOCHEMISTRY AND MICROBIOLOGY
Volume 50, Issue 6, Pages 637-643Publisher
PLEIADES PUBLISHING INC
DOI: 10.1134/S0003683814060027
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Funding
- federal special program Research and Technological Development in Russia [14.574.21.0062]
- Russian Foundation for Basic Research [13-07-12052 OFI_M]
- Ministry of Education and Science of the Russian Federation
- President of the Russian Federation [14.Z56.14.330-MK]
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Acetic acid bacteria Gluconobacter oxydans subsp. industrius RKM V-1280 were immobilized into a synthetic matrix based on polyvinyl alcohol modified with N-vinylpyrrolidone and used as biocatalysts for the development of bioanodes for microbial fuel cells. The immobilization method did not significantly affect bacterial substrate specificity. Bioanodes based on immobilized bacteria functioned stably for 7 days. The maximum voltage (fuel cell signal) was reached when 100-130 mu M of an electron transport mediator, 2,6-dichlorophenolindophenol, was added into the anode compartment. The fuel cell signals reached a maximum at a glucose concentration higher than 6 mM. The power output of the laboratory model of a fuel cell based on the developed bioanode reached 7 mW/m(2) with the use of fermentation industry wastes as fuel.
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