Article
Gastroenterology & Hepatology
Orna Gorelik, Alona Rogad, Lara Holoidovsky, Michael M. Meijler, Neta Sal-Man
Summary: The number of co-infections or multiple infections in reported diarrheal samples is increasing, possibly due to advances in bacterial diagnostic techniques. Vibrio cholerae and enteropathogenic Escherichia coli (EPEC) are commonly detected bacterial species in these samples, associated with high mortality rates. This study investigated the interspecies bacterial communication between EPEC and V. cholerae in the presence of indole, a major microbiome-derived metabolite found in the human gut. The results suggest that indole impairs the ability of EPEC to enhance its virulence activity in response to V. cholerae, and the composition of the microbiome or diets that influence indole gut concentrations may differentially impact pathogen virulence and their ability to sense and respond to competing bacteria.
Article
Biochemistry & Molecular Biology
Manuel Maestre-Reyna, Wei-Cheng Huang, Wen-Jin Wu, Praveen K. Singh, Raimo Hartmann, Po-Hsun Wang, Cheng-Chung Lee, Takaaki Hikima, Masaki Yamamoto, Yoshitaka Bessho, Knut Drescher, Ming-Daw Tsai, Andrew H-J Wang
Summary: Vibrio cholerae switches from biofilm-bound state to planktonic state through an autocatalytic proteolysis process involving the major biofilm scaffolding protein RbmA, which is triggered by phosphate-dependent induced proximity activation mechanism. Mutants of RbmA that are defective in autoproteolysis lead to larger and mechanically stronger biofilms, indicating a new sensory role of this biofilm scaffolding element affecting microbial community homeostasis and rheological properties.
Article
Biotechnology & Applied Microbiology
Jant Cres Caigoy, Toshi Shimamoto, Asish Kumar Mukhopadhyay, Sumio Shinoda, Tadashi Shimamoto
Summary: We investigated the impact of hapR sequence mutations on biofilm formation in Vibrio cholerae. Our results demonstrate that HapR mutations strongly influence biofilm formation, with sequence polymorphisms disrupting DNA-binding sites or dimerization of HapR resulting in more robust biofilms in V. cholerae.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Melanie Mindt, Lenny Ferrer, Dirk Bosch, Katarina Cankar, Volker F. Wendisch
Summary: Indole has gained increasing interest in the flavor and fragrance industry due to its distinct floral odor. However, the current production methods are non-sustainable and unprofitable. To offer an alternative approach, biosynthesis of indole has been studied, including glucose-based production using genetically-engineered strains.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Immunology
Jennifer Y. Cho, Rui Liu, Ansel Hsiao
Summary: The commensal microbes of the gut microbiota play an important role in defending against gastrointestinal pathogens. Different commensal communities contribute to individual differences in susceptibility to infections. This study reveals the different response mechanisms of susceptible and infection-resistant microbial communities to Vibrio cholerae infection, including differential gene expression related to oxidative stress and biofilm formation.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Biology
Kelsey Barrasso, Denise Chac, Meti D. Debela, Catherine Geigel, Anjali Steenhaut, Abigail Rivera Seda, Chelsea N. Dunmire, Jason B. Harris, Regina C. Larocque, Firas S. Midani, Firdausi Qadri, Jing Yan, Ana A. Weil, Wai-Leung Ng, Melanie Blokesch
Summary: Recent studies suggest that the presence of Paracoccus aminovorans, a commensal bacterium, in the human intestine may enhance the colonization of Vibrio cholerae. The interaction between the two species was shown to form a dual-species biofilm structure with novel features, and this interaction was found to be dependent on the Vibrio exopolysaccharide and other major components of mature V. cholerae biofilm. These findings demonstrate that multispecies biofilm formation could be a mechanism used by gut microbes to increase the virulence of pathogens and potentially impact the outcomes of enteric infections.
Article
Virology
Xu Li, Xiaorui Li, Huayao Zhang, Biao Kan, Fenxia Fan
Summary: Cholera, caused by pathogenic Vibrio cholerae, poses a significant public health risk through water and food transmission. Biofilm-associated V. cholerae plays a crucial role in seasonal cholera outbreaks as both a reservoir in aquatic environments and a direct source of human infection. High salt concentration combined with VP3 significantly improves the elimination of biofilm-associated V. cholerae.
Article
Biochemistry & Molecular Biology
Andrew A. Bridges, Jojo A. Prentice, Chenyi Fei, Ned S. Wingreen, Bonnie L. Bassler
Summary: This study quantitatively captures the mechanism by which bacteria use cyclic diguanylate (c-di-GMP) to transduce sensory information and regulate gene expression in biofilms. It shows that the bifunctional polyamine receptor NspS-MbaA enables local signaling and sensitivity to polyamines, which in turn regulates biofilm formation.
Article
Microbiology
Joao P. Pombo, Stephan P. Ebenberger, Anna M. Mueller, Heimo Wolinski, Stefan Schild
Summary: Vibrio cholerae represses 192 genes during biofilm formation, which are involved in metabolism, regulation, surface association, transmembrane transport, motility, and chemotaxis. These genes are not essential for biofilm formation and their presence can interfere with the ordered development of biofilms.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Clemence Abriat, Olivier Gazil, Marie-Claude Heuzey, France Daigle, Nick Virgilio
Summary: Silver nanoparticles (AgNPs) exhibit increased tolerance within mature biofilms, requiring a concentration nine times higher for elimination compared to planktonic cells. Mutant strains unable to form a pellicle biofilm are four times more susceptible to AgNPs than the wild-type strain. Electron microscopy analysis shows that AgNPs interact with extracellular matrix components and disrupt its microstructure. Major proteins such as Bap1 and RbmA mediate biofilm bacterial resistance to AgNPs.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Microbiology
Laura Alvarez, Sara B. Hernandez, Felipe Cava
Summary: The cell wall of most bacteria, composed of peptidoglycan, plays a crucial role in protecting them from environmental stressors. Vibrio cholerae has become a major model for studying cell wall genetic determinants, regulatory links, and adaptive mechanisms, shedding light on novel insights. This review provides a comprehensive overview of V. cholerae's cell wall biology and genetics, emphasizing the similarities and differences with Escherichia coli.
ANNUAL REVIEW OF MICROBIOLOGY, VOL 75, 2021
(2021)
Article
Biochemistry & Molecular Biology
Klaudyna Krause, Agnieszka Pyrczak-Felczykowska, Monika Karczewska, Magdalena Narajczyk, Anna Herman-Antosiewicz, Agnieszka Szalewska-Palasz, Dariusz Nowicki
Summary: Plant-derived phytoncides, isothiocyanates, have shown promising antibacterial potential against Vibrio cholerae, inhibiting bacterial growth, reducing toxicity, and inducing global stress response, suggesting they could be considered as effective antibacterial agents in V. cholerae infections.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Microbiology
Heidi A. Butz, Alexandra R. Mey, Ashley L. Ciosek, Alexander A. Crofts, Bryan W. Davies, Shelley M. Payne
Summary: CsrA is a critical posttranscriptional global regulator in Vibrio cholerae, controlling 22% of the bacterial transcriptome involving various cellular processes. Through RNA coimmunoprecipitation experiments, it was found that CsrA binds to multiple mRNAs encoding regulatory proteins, including major sigma factors. Additionally, CsrA directly binds to virulence gene transcripts, positively regulating the production of important virulence factors.
Article
Cell Biology
Jongoh Shin, Kevin Rychel, Bernhard O. Palsson
Summary: Vibrio natriegens regulates natural competence through the TfoX and QstR transcription factors, involving external DNA capture and transport. By using a machine-learning approach, this study decomposes the transcriptome of Vibrio natriegens into 45 independently modulated sets of genes (iModulons), revealing the repression of two housekeeping iModulons and the activation of six iModulons, including TfoX and QstR, a novel iModulon of unknown function, and three housekeeping iModulons. Phenotypic screening confirms that loss of iModulon function reduces or eliminates competence. These findings provide the genetic basis for understanding the competency in this organism.
Article
Immunology
Papiya Saha, Anirban Banerjee, Goutam Banerjee, Prasanta K. Bag
Summary: This study evaluated the antibacterial and antibiofilm activities of Typhonium trilobatum leaves extract against multi-drug resistant Vibrio cholerae. It demonstrated potential for the treatment of cholera and diarrheal patients, as it inhibited the toxin production and gene expression of the bacteria.
MICROBIAL PATHOGENESIS
(2022)
Article
Fisheries
Jetnaphang Kongrueng, Natta Tansila, Pimonsri Mitraparp-arthorn, Mitsuaki Nishibuchi, Gary J. Vora, Varaporn Vuddhakul
AQUACULTURE INTERNATIONAL
(2015)
Article
Biotechnology & Applied Microbiology
Junthip Thongjun, Natta Tansila, Kanda Panthong, Somporn Tanskul, Mitsuaki Nishibuchi, Varaporn Vuddhakul
ANNALS OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Taiyeebah Nuidate, Natta Tansila, Suwat Saengkerdsub, Jetnaphang Kongreung, Dhamodharan Bakkiyaraj, Varaporn Vuddhakul
INDIAN JOURNAL OF MICROBIOLOGY
(2016)
Article
Biotechnology & Applied Microbiology
Natta Tansila, Kristian Becker, Chartchalerm Isarankura-Na-Ayudhya, Virapong Prachayasittikul, Leif Bulow
BIOTECHNOLOGY LETTERS
(2008)
Article
Biochemistry & Molecular Biology
Uraiwan Thongchankaew, Pimonsri Mittraparp-arthorn, Pharanai Sukhumungoon, Natta Tansila, Taiyeebah Nuidate, Mitsuaki Nishibuchi, Varaporn Vuddhakul
CANADIAN JOURNAL OF MICROBIOLOGY
(2011)
Article
Automation & Control Systems
Chanin Nantasenamat, Kakanand Srungboonmee, Saksiri Jamsak, Natta Tansila, Chartchalerm Isarankura-Na-Ayudhya, Virapong Prachayasittikul
CHEMOMETRICS AND INTELLIGENT LABORATORY SYSTEMS
(2013)
Article
Biotechnology & Applied Microbiology
Thanachaporn Yaikhan, Manatsanan Chuerboon, Natchapol Tippayatham, Nateekarn Atimuttikul, Taiyeebah Nuidate, Mingkwan Yingkajorn, Aung Win Tun, Hansuk Buncherd, Natta Tansila
INDIAN JOURNAL OF MICROBIOLOGY
(2019)
Article
Chemistry, Analytical
Pongsakorn Thawornpan, Watthanachai Jumpathong, Supinya Thanapongpichat, Natta Tansila, Aung Win Tun, Luitzen de Jong, Hansuk Buncherd
Summary: The magnetic fraction of fly ash was proven to be an effective magnetic solid-phase extraction adsorbent for the enrichment of phosphoproteins. It has a high enrichment factor, is simple to use, and can be reused multiple times.
ANALYTICAL LETTERS
(2021)
Article
Biotechnology & Applied Microbiology
Khotchawan Bangpanwimon, Pimonsri Mittraparp-arthorn, Kanchana Srinitiwarawong, Natta Tansila
Summary: More than half the world's population is thought to be infected with Helicobacter pylori, which can cause gastric ulcers and deadly gastric cancer. This study presents a highly specific and sensitive noninvasive assay for detecting H. pylori in saliva samples, offering a potential alternative to current invasive diagnostic methods.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Multidisciplinary Sciences
Panida Paopradit, Natta Tansila, Komwit Surachat, Pimonsri Mittraparp-arthorn
Summary: The study found that the CFCS and extract of V. alginolyticus BC25 showed significant anti-QS activity against VPAHPND, affecting its biofilm formation and virulence, while enhancing disease resistance in shrimp against VPAHPND infection.
Article
Chemistry, Analytical
Charinrat Saechan, Pongsakorn Thawornpan, Supinya Thanapongpichat, Sawitree Hongmanee, Kanitta Srinoun, Aung Win Tun, Natta Tansila, Watthanachai Jumpathong, Hansuk Buncherd
Summary: Fly ash was used as a heterogeneous Fenton catalyst to successfully degrade methylene blue in aqueous solution. The influence of key operating parameters on the degradation efficiency was studied and the optimal degradation conditions were determined. Fly ash demonstrated acceptable reusability. The results indicated that fly ash is a cost-effective catalyst for the degradation of organic dyes in aqueous solution by a heterogeneous Fenton process.
ANALYTICAL LETTERS
(2023)
Article
Microbiology
Paramaporn Rattanaphan, Pimonsri Mittraparp-Arthorn, Kanitta Srinoun, Varaporn Vuddhakul, Natta Tansila
FEMS MICROBIOLOGY LETTERS
(2020)
Proceedings Paper
Biochemistry & Molecular Biology
Taiyeebah Nuidate, Natta Tansila, Kanda Panthong, Varaporn Vuddhakul
MOLECULAR AND CELLULAR LIFE SCIENCES: INFECTIOUS DISEASES, BIOCHEMISTRY AND STRUCTURAL BIOLOGY 2015 CONFERENCE
(2016)
Article
Biotechnology & Applied Microbiology
Chartchalerm Isarankura-Na-Ayudhya, Natta Tansila, Apilak Worachartcheewan, Leif Bulow, Virapong Prachayasittikul
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2010)