Article
Biotechnology & Applied Microbiology
Worarat Kruasuwan, Aekkachai Puseenam, Somjit Am-in, Srisakul Trakarnpaiboon, Warasirin Sornlek, Kanokarn Kocharin, Sasitorn Jindamorakot, Sutipa Tanapongpipat, Feng-Yan Bai, Niran Roongsawang
Summary: In this study, four S. cerevisiae strains with outstanding ethanol production at high temperatures were obtained from 150 strains screened. Among them, strain TBRC 12151 exhibited high ethanol tolerance at 40 °C and showed potential for use in industrial ethanol fermentation using cassava starch hydrolysate. This strain produced 65 g/L of ethanol from cassava starch hydrolysis at 40 °C.
Article
Biochemistry & Molecular Biology
Maria Mavrommati, Seraphim Papanikolaou, George Aggelis
Summary: This study describes an Adaptive Laboratory Evolution (ALE) strategy to develop Saccharomyces cerevisiae populations with increased tolerance to ethanol. The evolved populations after 100 generations could survive for 1 hour at 23% v/v ethanol and for 2 hours at 25% v/v ethanol. The fermentation ability and growth rate of the yeast strains were significantly improved through ethanol-based evolution.
PROCESS BIOCHEMISTRY
(2023)
Article
Agricultural Engineering
Ya Zhu Wang, Jin Zheng, Muhammad Nawaz, Fan Yang, Jiajun Hu, Min-Tian Gao
Summary: This study discovered that soluble polysaccharides from rice straw were conjugated with high levels of phenolic compounds. These conjugates significantly affected yeast growth and ethanol fermentation, improving their efficiency. The conjugates reduced osmotic stress and intracellular hydrogen peroxide levels, leading to higher yeast growth and ethanol production.
INDUSTRIAL CROPS AND PRODUCTS
(2022)
Article
Biotechnology & Applied Microbiology
Camila S. Varize, Augusto Bucker, Lucas D. Lopes, Renata M. Christofoleti-Furlan, Mariane S. Raposo, Luiz C. Basso, Boris U. Stambuk
Summary: The stress caused by ethanol on Saccharomyces cerevisiae cells is a limiting factor in industrial fuel ethanol production. This study focuses on the effects of genetic modifications on ethanol tolerance in an industrial yeast strain. The results show that one modification method improves fermentation performance under industrial conditions, leading to increased production of industrial fuel ethanol.
FERMENTATION-BASEL
(2022)
Article
Agricultural Engineering
Pei-Liang Ye, Xue-Qing Wang, Bing Yuan, Chen-Guang Liu, Xin-Qing Zhao
Summary: In this study, integrated analysis of transcriptome, proteome, and phosphoproteome revealed significant changes in protein kinases in yeast cells under acetic acid stress. Overexpression of the mitogen-activated protein kinase Hog1 improved ethanol production under stress conditions.
BIORESOURCE TECHNOLOGY
(2022)
Article
Agronomy
Heeyoung Park, Sang Un Park, Byeong-Kwan Jang, Jeong Jae Lee, Yong Suk Chung
Summary: Kenaf, an annual fiber crop mainly grown in India and China, is emerging as a promising bio-based energy source due to its rapid growth, CO2 absorption capabilities, and high productivity. The study evaluated 10 different cultivars of kenaf for cellulosic ethanol production, finding that ethanol yield and productivity were significantly impacted by cultivar type, rather than biomass composition. Engineering yeast to utilize xylose under pH control can maximize ethanol production from kenaf.
GLOBAL CHANGE BIOLOGY BIOENERGY
(2021)
Article
Biochemistry & Molecular Biology
Jasmina Masten Rutar, Berta Cillero-Pastor, Ronny Mohren, Natasa Poklar Ulrih, Nives Ogrinc, Polona Jamnik
Summary: This study investigated the antioxidant effects of fermented and non-fermented Spirulina extracts on yeast cells at the proteome level. The results showed that fermented extracts upregulated differentially expressed proteins while non-fermented extracts downregulated most proteins involved in cellular processes. Additionally, lactic acid fermentation contributed to a higher downregulation of stress response proteins in the cells.
Article
Biochemistry & Molecular Biology
Areej Elmheidi Elbakush, Didem Guven
Summary: This study demonstrated that adapted Saccharomyces cerevisiae has higher levels of trehalose and glycogen, leading to improved fermentation stress tolerance, higher ethanol productivity, and ethanol titer compared to wild strains. The study contributes to understanding the cellular response of yeast cells to fermentation stresses and highlights the advantages of using FT-IR spectroscopy for determining storage compounds.
PROCESS BIOCHEMISTRY
(2021)
Article
Biotechnology & Applied Microbiology
Wiwan Samakkarn, Khanok Ratanakhanokchai, Nitnipa Soontorngun
Summary: The transcription factor Znf1 plays a central role in activating genes essential for ethanol stress response in yeast cells, protecting membrane integrity and regulating cell wall biosynthesis and unfolded protein response. Understanding the role of Znf1 is crucial for enhancing stress tolerance and ethanol production in yeast strains used in various industries.
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Grzegorz Klosowski, Dawid Mikulski
Summary: The study evaluated the specific cellular response of Saccharomyces cerevisiae Ethanol Red strain to stress caused by toxic by-products during the pretreatment of lignocellulose. Results showed that yeast cells overproduced ergosterol and trehalose in the presence of 5-hydroxymethylfurfural under aerobic conditions, while a decrease in the biosynthesis of these indicators was observed under anaerobic conditions. Yeast strain was able to completely metabolize 5-hydroxymethylfurfural, furfural, syringaldehyde, and vanillin, with overproduction of Hsp60 in response to furan aldehydes.
Article
Agricultural Engineering
Fengguang Zhao, Yaping Zhang, Jian Hu, Ce Shi, Xiang Ao, Shengding Wang, Ying Lin, Zhongwei Sun, Shuangyan Han
Summary: A haploid gene-deletion library was screened to identify 22 vanillin-tolerant mutants and investigate the tolerance mechanisms. The deleted genes in these mutants were found to be involved in phosphate and inositol polyphosphate metabolism and intracellular sterol transport. Activation of the phosphate signaling pathway was unfavorable for yeast under vanillin pressure, while inositol polyphosphates played a role in altering vanillin tolerance by regulating phosphate metabolism. Additionally, reducing sterol transport from the plasma membrane enhanced tolerance to vanillin. Two representative yeast deletions, pho84 Delta and lam3 Delta, showed good growth performance and rapid ethanol production in the presence of vanillin.
BIORESOURCE TECHNOLOGY
(2023)
Article
Chemistry, Applied
Hideaki Tokuyama, Ryo Aoyagi, Kazuto Fujita, Yuki Maekawa, Shohei Riya
Summary: Ethanol fermentation using a hybrid system containing free and immobilized yeast cells in macroporous hydrogel scaffold was studied, with immobilization achieved through NIPA and MTGA hydrogels. The hybrid system showed higher ethanol productivity than the control system, with macroporous hydrogel allowing stable growth of immobilized yeast cells. In steady-state fermentation, the hybrid system had significantly higher cell densities and ethanol productivities compared to the control system.
REACTIVE & FUNCTIONAL POLYMERS
(2021)
Review
Energy & Fuels
Jian-Ren Xu, Muhammad Aamer Mehmood, Lan Wang, Niaz Ahmad, Hai-Jun Ma
Summary: This article discusses the research progress in stress-responsive factors of S. cerevisiae using multi-OMICs approaches and highlights how to increase tolerance and production yield of metabolic-engineered strains in the lignocellulose-to-ethanol conversion process.
FRONTIERS IN ENERGY RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Li Wang, Bo Li, Shi-Peng Wang, Zi-Yuan Xia, Min Gou, Yue-Qin Tang
Summary: This study successfully obtained a multiple stress-tolerant flocculating industrial S. cerevisiae strain E-158 using ARTP mutagenesis combined with genome shuffling and hybridization, which showed better growth under various stress conditions and improved antioxidant defense ability compared to the original strain.
PROCESS BIOCHEMISTRY
(2021)
Review
Biotechnology & Applied Microbiology
Daniel Gomes, Mariana Cruz, Miriam de Resende, Eloizio Ribeiro, Jose Teixeira, Lucilia Domingues
Summary: The constant growth of the worldwide industry has raised concerns about greenhouse gas emissions, leading to efforts in transitioning to cleaner biotechnological processes. Bioethanol production, particularly through VHG fermentation, plays a significant role in improving process economics. Several obstacles remain, but potential improvements in fermentation organisms, culture media, and process conditions are being explored for future advancements in VHG technologies.
FERMENTATION-BASEL
(2021)
Article
Cell Biology
Hatice Burcu Sisli, Taha Bartu Hayal, Selinay Senkal, Binnur Kiratli, Derya Sagrac, Selin Seckin, Murat ozpolat, Fikrettin Sahin, Bayram Yilmaz, Aysegul Dogan
Summary: The study suggests that APLNR can protect the hypothalamus from oxidative stress and promote GnRH cell proliferation and release. APLNR activation initiates a proliferative response and blocks apoptosis.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Guluna Erdem Koc, Alpaslan Gokcimen, Fikrettin Sahin
Summary: Liver fibrosis is a global public health problem that can lead to life-threatening complications. Activation of hepatic stellate cells is a key step in liver fibrosis. This study investigated the effect of boron compound-treated foreskin-derived mesenchymal stem cells on liver fibrosis.
BIOLOGICAL TRACE ELEMENT RESEARCH
(2023)
Article
Agronomy
Adem Gunes, Leyla Tarhan, Bahar Sogutmaz Oezdemir, Metin Turan, Fikrettin Sahin
Summary: In this study, Plant Growth Promoting Rhizobacteria (PGPR) bacteria were applied to the soil to improve the yield parameters of tomato plants, as excessive and unconscious use of chemical fertilizers in tomato cultivation can lead to economic and environmental issues such as loss of soil fertility and pollution.
COMMUNICATIONS IN SOIL SCIENCE AND PLANT ANALYSIS
(2023)
Article
Chemistry, Physical
Gizem Erensoy, Kai Ding, Chang-Guo Zhan, Gamze Ciftci, Kemal Yelekci, Merve Duracik, Ozlem Bingol Ozakpinar, Esra Aydemir, Zubeyde Nur Yilmaz, Fikrettin Sahin, Necla Kulabas, Esra Tatar, Ilkay Kucukguzel
Summary: mPGES-1 is an important enzyme involved in PGE2 synthesis and its inhibition holds promise for the discovery of anti-inflammatory and anti-cancer drugs. The design of mPGES-1 inhibitors can provide better control of PGE2-associated diseases compared to traditional drugs with serious side effects. However, no mPGES-1 inhibitors have reached the clinical market yet, highlighting the need for the development of novel inhibitors with suitable drug-like properties.
JOURNAL OF MOLECULAR STRUCTURE
(2023)
Article
Medicine, Research & Experimental
Nese Basak Turkmen, Hande Yuce, Muhterem Aydin, Asli Taslidere, Aysegul Dogan, Dilan Askin Ozek, Taha Bartu Hayal, Seyma Yasar, Osman Ciftci, Songul Unuvar
Summary: Based on the experimental results, it can be deduced that nerolidol has a significant ameliorating effect on lipid peroxidation, oxidative stress, inflammation, histopathological damage, and apoptosis accompanying PCOS in female rats.
Article
Biochemistry & Molecular Biology
Esra Aydemir, Emre Can Tuysuz, Omer Faruk Bayrak, Didem Tecimel, Aysen Asli Hizli-Deniz, Fikrettin Sahin
Summary: In this study, gene silencing of eEF2K was found to result in a slight increase in cellular proliferation and a decrease in death rates in chordoma cells. Additionally, no alteration in the sensitivity of chordoma cells to chemotherapy was observed, but suppression of cell migratory and invasion-related properties was promoted.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Zeynep Busra Bolat, Zeynep Islek, Fikrettin Sahin, Mehmet Hikmet Ucisik
Summary: This study evaluated the therapeutic potential of a new type of curcumin-loaded emulsome nanoparticular system, CurcuEmulsomes, in androgen dependent prostate cancer cell line LNCaP. The results showed that CurcuEmulsomes had a more effective inhibitory effect on the proliferation of LNCaP cells compared to free curcumin. CurcuEmulsomes not only prolonged the biological activity of curcumin but also induced apoptotic cell death and cell cycle arrest. This highlights the potential of CurcuEmulsomes to enhance the intrinsic anticancer activities of curcumin in androgen dependent prostate cancer therapy.
MOLECULAR BIOLOGY REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Bahadir Bulbul, Kai Ding, Chang-Guo Zhan, Gamze Ciftci, Kemal Yelekci, Merve Gurboga, Ozlem Bingol Ozakpinar, Esra Aydemir, Deniz Baybag, Fikrettin Sahin, Necla Kulabas, Sinem Helvacioglu, Mohammad Charehsaz, Esra Tatar, Suheyla Ozbey, Ilkay Kucukguzel
Summary: In this study, novel triazole-bearing ketone and oxime derivatives were synthesized from Ibuprofen, and their cytotoxic activities against cancer cells were evaluated. Some compounds showed anti-inflammatory and angiogenesis inhibitory activities without toxicity, suggesting their potential as promising agents in anti-inflammatory and cancer treatments.
MOLECULAR DIVERSITY
(2023)
Article
Cell Biology
Hatice Burcu Sisli, Selinay Senkal, Taha Bartu Hayal, Ezgi Bulut, Aysegul Dogan
Summary: Mesoderm-derived cells have great potential for regenerative therapy. Understanding the signaling pathways involved in their differentiation is important. This study revealed that Apelin receptor (Aplnr) signaling induces cell migration and enhances mesoderm differentiation from mouse embryonic stem cells (mESCs) through the FGF/MAPK pathway. Apelin-13 treatment improved MSC characteristics and enhanced cell proliferation and survival during MSC derivation from mesoderm.
Article
Clinical Neurology
Esra Aydemir, Nur Zubeyda Yilmaz, Omer Faruk Bayrak, Fikrettin Sahin
Summary: Objective Interaction between tumor cells and the surrounding environment is crucial for tumor growth and metastasis. Exosomes, also known as extracellular vesicles, play a crucial role in signaling between tumor cells and their microenvironment. Tumor-derived exosomes have protumorigenic effects such as metastasis, hypoxia, angiogenesis, and epithelial-mesenchymal transition.
JOURNAL OF NEUROLOGICAL SURGERY PART B-SKULL BASE
(2023)
Article
Biochemistry & Molecular Biology
Hatice Burcu Sisli, Taha Bartu Hayal, Selinay Senkal, Ezgi Bulut, Binnur Kiratli, Ayla Burcin Asutay, Fikrettin Sahin, Omer Faruk Bayrak, Aysegul Dogan
Summary: This study demonstrated the negative regulatory effect of the Wnt signaling pathway on proliferation and migration capacity of human chordoma cells. Activation of the Wnt pathway led to cell cycle arrest, reduced migration potential, and cell death. These findings suggest that the Wnt pathway plays a key role in suppressing the invasive and proliferative characteristics of human chordoma cells and has potential as a therapeutic target for further clinical studies.
CELL BIOCHEMISTRY AND BIOPHYSICS
(2023)
Article
Biochemistry & Molecular Biology
Fikrettin Sahin, Mohammad Bagher Pirouzpanah, Azizeh Farshbaf-Khalili, Erhan Aysan, Aysegul Dogan, Selami Demirci, Alireza Ostadrahimi, Majid Mobasseri
Summary: This study compared the therapeutic effect of a sodium pentaborate-based topical formulation with a conventional remedy on diabetic foot ulcers. The results showed that patients who used sodium pentaborate gel had lower ulcer grades, higher cure rates, and lower recurrence rates. This suggests that sodium pentaborate gel can effectively treat diabetic foot ulcers.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Taha Bartu Hayal, Aysegul Dog, Selinay Senkal, Ezgi Bulut, Hatice Burcu Sisli, Fikrettin Sahin
Summary: This study aimed to investigate the role of boron compounds in heart development and the effect on the pluripotency of mouse embryonic stem cells for the first time. The results showed that boron treatment at nontoxic concentrations maintained the pluripotency of embryonic stem cells and triggered endodermal and mesodermal fate determination. Additionally, boron derivative treatments initiated cardiomyocyte differentiation and increased the expression levels of cardiac differentiation related genes.
JOURNAL OF TRACE ELEMENTS IN MEDICINE AND BIOLOGY
(2023)
Article
Materials Science, Biomaterials
Hatice Burcu Sisli, Selinay Senkal Turhan, Ezgi Bulut, Fikrettin Sahin, Aysegul Dogan
Summary: Stem cells are valuable resources for personalized medicine, and mesenchymal stem cells (MSCs) have been recognized as safe, ethical, and accessible therapeutic tools due to their immunomodulatory and controlled differentiation properties. The Apelin receptor (Aplnr) signaling pathway has been found to play a crucial role in embryonic development and stem cell differentiation, promoting mesoderm commitment and enhancing the characteristics and differentiation capacity of MSCs. These findings provide an important foundation for personalized cell therapy.
Article
Chemistry, Physical
Atilla Topcu, Zekai Halici, Elif Cadirci, Emre Karakus, Aysegul Dogan, Feray Bakan, Yapincak Goncu, Nuran Ay, Fikrettin Sahin
Summary: This study investigated the effects of incorporating different concentrations of boron nitride and/or hydroxyapatite into poly-(lactide-co-glycolide) (PLGA) scaffolds on bone defects in osteoporotic rats. Results showed that bone defects were healed in the groups treated with PLGA scaffolds containing boron nitride and/or hydroxyapatite, with the 2.5% boron nitride + 10% hydroxyapatite group showing the best healing.
JOURNAL OF MOLECULAR STRUCTURE
(2024)
