Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 175, Issue 4, Pages 1971-1980Publisher
SPRINGER
DOI: 10.1007/s12010-014-1379-z
Keywords
Giant African snail; Gastrointestinal tract; Cellulolytic bacteria; Enrichment; 16S rRNAclone library
Funding
- University Grant Commission (UGC), Government of India
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The cellulolytic bacterial community structure in gastrointestinal (GI) tract of Achatina fulica was studied using culture-independent and - dependent methods by enrichment in carboxymethyl cellulose (CMC). Culture-dependent method indicated that GI tract of snail was dominated by Enterobacteriaceae members. When tested for cellulase activities, all isolates obtained by culture-dependent method showed both or either of CMCase or avicelase activity. Isolate identified as Citrobacter freundii showed highest CMCase and medium avicelase activity. Sequencing of clones from the 16S rRNA gene clone library identified ten operational taxonomic units (OTUs), which were affiliated to Enterobacteriaceae of phylum Gammaproteobacteria. Of these ten OTUs, eight OTUs closely matched with Enterobacter and Klebsiella genera. The most abundant OTU allied to Klebsiella oxytoca accounted for 70 % of the total sequences. The members of Klebsiella and Enterobacter were observed by both methods indicating their dominance among the cellulolytic bacterial community in the GI tract of the snail.
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