Impact of Gene Dosage on the Production of Lipase from Rhizopus chinensis CCTCC M201021 in Pichia pastoris

High yield Rhizopus chinenisis prolipase production in Pichia pastoris: Impact of methanol concentration

(2011) Dan Wu et al.   BIOTECHNOLOGY AND BIOPROCESS ENGINEERING

Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris

(2011) Kristina Nordén et al.   BMC BIOTECHNOLOGY

Engineering of protein secretion in yeast: strategies and impact on protein production

(2010) Alimjan Idiris et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

An improved method for enhanced production and biological activity of human secretory leukocyte protease inhibitor (SLPI) in Pichia pastoris

(2010) Zhiguo Li et al.   BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS

Real-time PCR-based determination of gene copy numbers inPichia pastoris

(2010) Sandra Abad et al.   Biotechnology Journal

Enhancing production of Yarrowia lipolytica lipase Lip2 in Pichia pastoris

(2010) Mingrui Yu et al.   ENGINEERING IN LIFE SCIENCES

Codon optimization through a two-step gene synthesis leads to a high-level expression of Aspergillus niger lip2 gene in Pichia pastoris

(2010) Jiangke Yang et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Lipase from Rhizomucor miehei as an industrial biocatalyst in chemical process

(2010) Rafael C. Rodrigues et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Accurate and objective copy number profiling using real-time quantitative PCR

(2010) Barbara D’haene et al.   METHODS

Novel minor lipase from Rhizopus chinensis during solid-state fermentation: Biochemical characterization and its esterification potential for ester synthesis

(2009) Shu Yang Sun et al.   BIORESOURCE TECHNOLOGY

Expression of Methyl Parathion Hydrolase in Pichia pastoris

(2009) Haojie Yu et al.   CURRENT MICROBIOLOGY

Directed gene copy number amplification inPichia pastorisby vector integration into the ribosomal DNA locus

(2009) Hans Marx et al.   FEMS YEAST RESEARCH

Efficient generation of multi-copy strains for optimizing secretory expression of porcine insulin precursor in yeastPichia pastoris

(2009) T. Zhu et al.   JOURNAL OF APPLIED MICROBIOLOGY

Expression and characterization of Bacillus subtilis PY22 α-amylase in Pichia pastoris

(2009) Barçın Karakaş et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Engineering the Pichia pastoris methanol oxidation pathway for improved NADH regeneration during whole-cell biotransformation

(2009) Kirsten Schroer et al.   METABOLIC ENGINEERING

Novel strategies for increased copy number and expression of recombinant human gelatin in Pichia pastoris with two antibiotic markers

(2008) Kim E. Williams et al.   ENZYME AND MICROBIAL TECHNOLOGY

Purification and biochemical characterization of an intracellular lipase byRhizopus chinensisunder solid-state fermentation and its potential application in the production of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA)

(2008) Shu Yang Sun et al.   JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY

Rhizopus chinensis lipase: Gene cloning, expression in Pichia pastoris and properties

(2008) Xiao-Wei Yu et al.   JOURNAL OF MOLECULAR CATALYSIS B-ENZYMATIC

Promoter library designed for fine-tuned gene expression in Pichia pastoris

(2008) Franz S. Hartner et al.   NUCLEIC ACIDS RESEARCH