Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 169, Issue 4, Pages 1329-1345Publisher
SPRINGER
DOI: 10.1007/s12010-012-0086-x
Keywords
Antimicrobial activity; DNA binding; DNA cleavage; SOD mimic; Cytotoxicity
Funding
- U.G.C.
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The square pyramidal copper(II) complexes of N, O- donor ligand and ciprofloxacin have been synthesized. Synthesized complexes were characterized by physicochemical parameters like elemental analysis, electronic, FT-IR and LC-MS spectra. The complexes were screened for their antimicrobial activity against Gram(+Ve), i.e. Staphylococcus aureus, Bacillus subtilis, and Gram(-Ve), i.e. Serratia marcescens, Pseudomonas aeruginosa and Escherichia coli, microorganisms in terms of minimum inhibitory concentration and colony-forming unit. To determine the binding mode of complexes with Herring Sperm DNA, absorption titration and viscosity measurement were employed. DNA cleavage activity was carried out by gel electrophoresis experiment using supercoiled form of pUC19 DNA. The complexes were tested for their superoxide dismutase mimic activity in terms of IC50 value. Synthesized complexes were also screened for their cytotoxicity using brine shrimp lethality assay method.
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