Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 166, Issue 7, Pages 1615-1625Publisher
SPRINGER
DOI: 10.1007/s12010-012-9538-6
Keywords
Clostridium butyricum; Acetone butanol ethanol (ABE); Oil palm empty fruit bunch (OPEFB); Anaerobic fermentation; Biomass
Funding
- Ministry of Science, Technology and Innovation (MOSTI), Malaysia
- Universiti Putra Malaysia
- Environmental Biotechnology Research Group
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Acetone-butanol-ethanol (ABE) production from renewable resources has been widely reported. In this study, Clostridium butyricum EB6 was employed for ABE fermentation using fermentable sugar derived from treated oil palm empty fruit bunch (OPEFB). A higher amount of ABE (2.61 g/l) was produced in a fermentation using treated OPEFB as the substrate when compared to a glucose based medium that produced 0.24 g/l at pH 5.5. ABE production was increased to 3.47 g/l with a yield of 0.24 g/g at pH 6.0. The fermentation using limited nitrogen concentration of 3 g/l improved the ABE yield by 64%. The study showed that OPEFB has the potential to be applied for renewable ABE production by C. butyricum EB6.
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