Article
Entomology
Mengmeng Zhang, Zhihao Cui, Nuo Zhang, Guanglin Xie, Wenkai Wang, Li Chen
Summary: The study found that beta-caryophyllene and hexanal emitted by peanut plants are strongly attractive to Holotrichia parallela, with a blend of these two compounds being the most effective attractant. Other identified compounds did not show synergistic effects on the attraction of the beetle.
Article
Biochemistry & Molecular Biology
Er-Tao Li, Han-Jia Wu, Jian-Hui Qin, Jing Luo, Ke-Bin Li, Ya-Zhong Cao, Shuai Zhang, Yu Peng, Jiao Yin
Summary: Holotrichia parallela is attracted to the volatiles released by organic fertilizers during oviposition. Odorant-binding protein 3 (HparOBP3) was identified as a key protein in the perception of oviposition cues. Silencing HparOBP3 significantly reduced the electro-physiological responses and tropism of males and females to specific compounds. The binding ability of HparOBP3 was dependent on hydrophobic residues, and its mutation diminished the binding ability. Silencing HparOBP3 also reduced the attraction and oviposition indexes of organic fertilizers to H. parallela.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Agronomy
Er-Tao Li, Han-Jia Wu, Zhi-Min Wang, Ke-Bin Li, Shuai Zhang, Ya-Zhong Cao, Jiao Yin
Summary: Through RNA sequencing, it was found that the PI3K/Akt/CncC pathway mediates the defense response of H. parallela larvae against EPN-Bt infection, and the expression of CarE18 is associated with this pathway. These findings provide valuable insights for the design of appropriate management strategies to combat this agricultural pest.
PEST MANAGEMENT SCIENCE
(2023)
Article
Agriculture, Multidisciplinary
Jia-Nan Liu, Jing-Hui Xi, Zhun Wang, Shi-Wen Zhao, Xiao Wang, Yun-Wei Bu, Ke-Xin Zhou, Yu Pan, Shang Wang
Summary: In this study, 31 glutathione S-transferases (GSTs) were identified from the antennal transcriptome of Holotrichia parallela, with HpGSTd1 showing the highest expression level. Recombinant HpGSTd1 was found to have degradation activity towards various unsaturated aldehyde volatiles, and the metabolite of cinnamaldehyde was identified using high-resolution mass spectrometry (HRMS). Molecular docking analysis and site-directed mutagenesis revealed the key residues of HpGSTd1 involved in odorant degradation. The unsaturated aldehyde volatiles also elicited behavioral and electrophysiological responses in H. parallela. These findings suggest that HpGSTd1 plays an essential role in inactivating odorants in this underground pest, providing new insights for identifying molecular targets and exploring effective olfactory regulators.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Zhongjun Gong, Jing Zhang, Yanmin Li, Huiling Li, Ziqi Zhang, Yifan Qin, Yueli Jiang, Yun Duan, Tong Li, Jin Miao, Yuqing Wu
Summary: Holotrichia parallela, an important plant pest, shows differential egg production, oviposition duration, and survival rate when feeding on different host plants. Transcriptome analysis reveals genes related to oviposition in beetles feeding on elm, willow, and purpus privet leaves. The identified genes, such as vitellogenesis and odorant-binding proteins, provide insights into the molecular mechanisms of oviposition in H. parallela. This study also presents a method for identifying potential genes for pest control.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Dan Zhao, Zhao-rui Liu, Han Wu, Chao-ran Fu, Ya-zi Li, Xiu-jun Lu, Wei Guo
Summary: Chitin deacetylases are crucial for insect survival by catalyzing chitin N-deacetylation to produce chitosan, making them important targets for insecticidal drug development. The study identified putative Group I chitin deacetylase genes in Holotrichia parallela and demonstrated their essential role in cuticle organization, providing a basis for potential new control strategies.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Ertao Li, Jianhui Qin, Honglin Feng, Jinqiao Li, Xiaofeng Li, Innocent Nyamwasa, Yazhong Cao, Weibin Ruan, Kebin Li, Jiao Yin
Summary: The study found that in response to nematode infection, immune-related genes such as PGRP-SC1, PPAE-I, and GST are involved in the immune response of Holotrichia parallela, with PGRP-SC1 and PPAE-I showing higher expression in the midgut and fat body, respectively, and GST exhibiting high expression in both. Silencing these genes increases susceptibility of H. parallela larvae to nematode infection.
Article
Biotechnology & Applied Microbiology
Er-Tao Li, Shuai Zhang, Ke-Bin Li, Innocent Nyamwasaa, Jin-Qiao Li, Xiao-Feng Li, Jian-Hui Qin, Jiao Yin
Summary: The study found that the Heterorhabditis beicherriana LF strain exhibited higher pathogenicity against Holotrichia parallela larvae, with good control effectiveness at optimal temperature and soil moisture. It was compatible with Bt (HBF18). Combinations of EPNs and Bt showed higher mortality rates for H. parallela larvae compared to using nematodes alone, particularly when Bt (HBF-18) was combined with EPNs at LC50 concentrations.
BIOLOGICAL CONTROL
(2021)
Article
Agronomy
Yu Pan, Zhun Wang, Shi-Wen Zhao, Xiao Wang, Yun-Shuo Li, Jia-Nan Liu, Shang Wang, Jing-Hui Xi
Summary: After Holotrichia parallela larva feeding, maize roots produced changes in phytohormones and volatiles, with tetradecane potentially serving as a potent defense induction signal that increases plant resistance to the larvae.
PEST MANAGEMENT SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Dan Zhao, Xiaomin Liu, Zhaorui Liu, Han Wu, Xiujun Lu, Wei Guo
Summary: Chitin metabolism enzymes, specifically NAG and NAGK, play important roles in the degradation and conversion of chitin. In this study, NAG and NAGK genes were identified and their spatiotemporal expression patterns were investigated in Holotrichia parallela larvae. Silencing of HpNAG and HpNAGK resulted in a significant RNAi effect and inhibited larval growth. The study also showed that silencing these genes affected the expression levels of chitin metabolism-related genes and reduced the thickness and chitin content of the cuticle.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2022)
Article
Agronomy
Jakub Dobrzynski, Barbara Wrobel, Ewa Beata Gorska
Summary: Cellulolytic enzymes produced by Bacillus sp. 8E1A, a spore-forming bacterium, may be a potential solution for the degradation of lignocellulosic waste. The highest production of carboxymethyl cellulase (CMCase), filter paper hydrolase (FPase), and microcrystalline cellulose Avicel hydrolase (Avicelase) by Bacillus sp. 8E1A was observed when using carboxymethyl cellulose, Avicel cellulose, and carboxymethyl cellulose, respectively. The optimal temperature and pH for the enzyme activity varied depending on the type of cellulase.
Article
Physiology
Jiankun Yi, Shang Wang, Zhun Wang, Xiao Wang, Gongfeng Li, Xinxin Zhang, Yu Pan, Shiwen Zhao, Juhong Zhang, Jing-Jiang Zhou, Jun Wang, Jinghui Xi
Summary: In this study, 20 candidate CXEs were identified in Holotrichia parallela, with some showing antenna-biased expression. The differential expression of CXEs in male and female antennae implies potential roles in odorant degradation, providing insights into the olfactory mechanism of H. parallela.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Environmental Sciences
Shuhe Chen, Miyu Haga, Ichiro Imai, Ryuichi Sakai, Masaki J. Fujita
Summary: In this study, a bacterial strain Go58 with potent algicidal activity against bloom-forming cyanobacterium was discovered. The strain is closely related to Pseudomonas protegens and produces pyoluteorins, which exhibit high toxicity against cyanobacteria. This bacterial group has the potential to be a safe biological countermeasure for controlling cyanobacterial blooms.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Genetics & Heredity
Jing Zhao, Feng Zhang, Jingjing Yuan, Zhenjie Du, Xuebin Qi
Summary: Holotrichia parallela is an important pest in China that causes economic losses for peanut, potato, and soybean. This study sequenced and analyzed its complete mitochondrial genome, revealing its conservation in gene order with other Scarabaeidae insects. Phylogenetic analysis showed a close relationship between H. parallela and Rhopaea magnicornis, Polyphylla gracilicornis, and Melolontha hippocastani.
MITOCHONDRIAL DNA PART B-RESOURCES
(2022)
Article
Entomology
Yafei Qu, Xiangyu Liu, Xu Zhao, Jianhui Qin, Yazhong Cao, Kebin Li, Jing-Jiang Zhou, Senshan Wang, Jiao Yin
Summary: This study cloned the odorant-binding protein gene HparOBP14, which is abundantly expressed in the scarab beetle Holotrichia parallela, and demonstrated its involvement in binding organic matter volatiles during egg-laying. Knockdown of HparOBP14 expression using RNA interference technique significantly reduced the electrophysiological and behavioral responses of female adults to certain organic matter volatiles, suggesting a potential role in oviposition behavior.