Article
Agricultural Engineering
Qianqian Yang, Lei Zhang, Zhina Lian, Junhua Zhang
Summary: In this study, lactic acid was used for XOS production from corncob, achieving a XOS yield of 69.9%. Additionally, cellulase hydrolysis of corncob residue resulted in 95.6% glucose and 54.0% xylose. Bacillus subtilis YS01 was successfully cultured using the cellulase hydrolysate, with a viable count of 6.4x10(8) CFU/mL and glucose and xylose utilization rates of 99.0% and 89.8%, respectively.
BIORESOURCE TECHNOLOGY
(2023)
Article
Environmental Sciences
Youqiang Xu, Xiao Liu, Jingrong Zhao, Huiqin Huang, Mengqin Wu, Xiuting Li, Weiwei Li, Xiaotao Sun, Baoguo Sun
Summary: The GRAS Bacillus subtilis strain BJQ0005 efficiently degraded phthalate esters, and its metabolic pathway and enzyme mechanism have been thoroughly studied. This provides important insights for solving phthalate ester pollution in the environment and food industry.
ENVIRONMENTAL POLLUTION
(2021)
Article
Biotechnology & Applied Microbiology
Xian Yin, Meiyi Zhao, Yu Zhou, Hulin Yang, Yonghong Liao, Fenghuan Wang
Summary: This study reports the significance of methylmethionine as a methyl donor for SeMCys production in B. subtilis, and highlights the importance of enhanced precursor supply in facilitating SeMCys synthesis. The results represent the highest SeMCys production to date and provide insights into Se metabolism.
MICROBIAL CELL FACTORIES
(2023)
Article
Chemistry, Multidisciplinary
Feixia Liu, Jie Ren, Lu Guo, Yang Liu, De-Feng Li, Bo Yu
Summary: This study successfully achieved the enantioselective synthesis of S-PGB by engineering d-hydantoinase, avoiding the generation of pollutants in the process. The best variant demonstrated a high conversion rate and chiral selectivity in kilogram scale synthesis, offering a novel green and efficient route for S-PGB production.
Article
Biotechnology & Applied Microbiology
Chun-Yue Weng, Dan-Na Wang, Shan-Yun Ban, Qiu-Yao Zhai, Xin-Yi Hu, Feng Cheng, Ya-Jun Wang, Yu-Guo Zheng
Summary: In this study, Protease 6SD was immobilized on multi-walled carbon nanotubes to achieve enantioselective resolution of (R,S)-1 to yield optically pure (S)-1, improving substrate solubility and catalyst recyclability.
JOURNAL OF BIOTECHNOLOGY
(2021)
Article
Food Science & Technology
Wenqiang Shen, Yaojun Liu, Xinyue Zhang, Xiong Zhang, Xiaoping Rong, Lihong Zhao, Cheng Ji, Yuanpei Lei, Fengjuan Li, Jing Chen, Qiugang Ma
Summary: In this study, the potential ameliorative effects of probiotic Bacillus subtilis and biodegradable Bacillus subtilis on ZEN toxicosis in gilts were compared. The results showed that the biodegradable Bacillus subtilis had significant protective effects on growth performance, immune function, hormone secretion, and ZEN elimination in gilts compared to the probiotic Bacillus subtilis.
Article
Multidisciplinary Sciences
Luyao Bao, Juan Hu, Bowen Zhan, Mingzhe Chi, Zhengyang Li, Sen Wang, Chan Shan, Zhaozhao Zhao, Yanchao Guo, Xiaoming Ding, Chaoneng Ji, Shengce Tao, Ting Ni, Xuelian Zhang, Guoping Zhao, Jixi Li
Summary: This study reports the crystal structures of RNase J from Mycobacterium tuberculosis in apo-state and complex with single strand RNA, revealing its physiological function in RNA metabolism.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Lusha Wei, Lei Liu, Ziwei Bian, Yifei Wu
Summary: In this study, the recombinant xylanase XynA was used to hydrolyze birchwood and beechwood xylans, and the polymerization distribution of xylo-oligosaccharides generated during enzymatic digestion was analyzed by MALDI-TOF-MS. Through enzyme hydrolysis, xylobiose and xylotriose were produced as potential food additives.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Sarah Gingichashvili, Doron Steinberg, Ronit Vogt Sionov, Osnat Feuerstein, Noa E. Cohen
Summary: In this study, computational analytics were used to quantify the morphological response of Bacillus subtilis to the proximity of an antimicrobial source. The results revealed that the inner core, characterized by wrinkled formations, is more preserved compared to the periphery. Additionally, a crescent-shaped colony morphology and changes in the growth substrate of macrocolonies exposed to the antimicrobial were observed.
SCIENTIFIC REPORTS
(2022)
Article
Chemistry, Analytical
Mei-yan Wu, Wen-wei Li, Graham Christie, Peter Setlow, Yong-qing Li
Summary: Using Raman tweezers and live-cell phase-contrast microscopy, this study characterized the lysis process of individual Bacillus subtilis cells carrying PBSX prophage during spores' germination, outgrowth, and vegetative growth. The findings showed that nucleic acid intensities in induced cultures decreased rapidly after prophage induction, with a delay before cell burst, and the latent time and burst time varied in cell populations under different culture conditions.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Qilin Yu, Haohao Mao, Bowen Yang, Yahui Zhu, Cheng Sun, Zhiqiang Zhao, Yang Li, Yaobin Zhang
Summary: Electrical stimulation was found to enhance microbial extracellular electron transfer (EET), but the underlying reasons were unclear. This study showed that Bacillus subtilis, a gram-positive bacterium capable of extracellular respiration, had a higher EET capacity after electrical domestication. It was discovered that the polarization of amide groups induced by electrical stimulation promoted H-bond recombination and radical generation of protein-like substances, facilitating extracellular electron transfer via proton-coupled mechanism.
Article
Chemistry, Multidisciplinary
Alice Miral, Sylvain Fournet, Catherine Porte, Aurelie Sauvager, Josselin Montarry, Sophie Tomasi, Sylvain Tranchimand
Summary: Healthy food is a major challenge for the development of this century, with plant-parasitic nematodes causing significant damage to global crop yields. The use of chemical nematicides for control is no longer environmentally sustainable, and biocontrol methods, such as biopesticides derived from living organisms, offer a promising alternative. This study focuses on the underexploited ecological niche of lichen microecosystems to develop new plant protection products. The lichen-associated bacterial suspensions from Paenibacillus etheri are investigated for their potential as nematicides against two nematode species, and the effects of volatile organic compounds produced by the bacteria are evaluated.
Article
Biochemistry & Molecular Biology
Zhiwei Tu, Henk L. Dekker, Winfried Roseboom, Bhagyashree N. Swarge, Peter Setlow, Stanley Brul, Gertjan Kramer
Summary: The research investigated the proteome dynamics during sporulation of Bacillus subtilis vegetative cells under nutrient limitation. Protein co-expression analysis identified four modules, with brown and green modules being upregulated during sporulation containing proteins associated with sporulation, while blue module showed negative correlation and contained ribosomal and metabolic proteins. Besides known transcription regulons, some proteins co-expressed with brown and green modules may also play roles during sporulation. Levels of some coat proteins decreased late in sporulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Environmental Sciences
Shanshan Di, Zhenzhen Liu, Huiyu Zhao, Ying Li, Peipei Qi, Zhiwei Wang, Hao Xu, Yuanxiang Jin, Xinquan Wang
Summary: The tire antidegradant 6PPD and its transformation product 6PPD-Q are highly toxic to aquatic organisms. Their toxicities are species-specific, and the enantiomers of 6PPD do not exhibit enantioselective hydrolysis or toxicity. The formation concentrations of 6PPD-Q in water solutions exceed the median lethal concentrations of certain fish species, raising concerns for environmental risk assessments.
ENVIRONMENT INTERNATIONAL
(2022)
Editorial Material
Biochemistry & Molecular Biology
Bing Wang, Irina Artsimovitch
Summary: Bacillus subtilis utilizes uncoupled transcription and translation strategies to meet its unique regulatory needs, showing that bacteria employ diverse gene expression strategies.
TRENDS IN MICROBIOLOGY
(2021)