Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 160, Issue 1, Pages 40-49Publisher
HUMANA PRESS INC
DOI: 10.1007/s12010-009-8586-z
Keywords
Enterococcus faecium; Enterocin LR/6; Purification; Characterization; Mode of action
Funding
- Council of Scientific and Industrial Research and the Department of Biotechnology, India
- UGC
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Enterocin LR/6, a bacteriocin obtained from the culture filtrate of Enterococcus faecium strain LR/6, has been purified to homogeneity using ammonium sulfate precipitation, cation-exchange chromatography, gel-filtration, and checked on reverse-phase high-performance liquid chromatography. It is active at high temperatures (boiling as well as autoclaving) and over a wide range of pH (2.0-8.0). Also, it is sensitive to a number of proteolytic enzymes but is stable in the presence of surfactants and organic solvents. The protein could be stored at least up to 1 year at low temperatures (4 degrees C and -20 degrees C) without any loss of activity. The N-terminal sequence of enterocin LR/6 showed no homology with known enterocins or other bacteriocins present in the database, suggesting it to be a novel enterocin. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry and tricine sodium dodecyl sulfate-polyacrylamide gel electrophoresis analysis revealed its mass to be similar to 6.1 kDa. It showed a bactericidal mode of action against indicator strain, Micrococcus luteus.
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