Journal
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
Volume 160, Issue 1, Pages 255-268Publisher
SPRINGER
DOI: 10.1007/s12010-009-8654-4
Keywords
Biobleaching; Penicillium oxalicum; Co-cultivation; Xylanase; Laccase; Pleurotus ostreatus
Funding
- Council of Scientific and Industrial Research, New Delhi, India
- All India Council of Technical Education, New Delhi, India
Ask authors/readers for more resources
Mixed enzyme preparation having both xylanase and laccase activity was evaluated for its bleach enhancing ability of mixed wood pulp. The enzyme was produced through co-cultivation of mutant Penicillium oxalicum SAU(E)-3.510 and Pleurotus ostreatus MTCC 1804 under solid-state fermentation. Bleaching of pulp with mixed enzyme had resulted into a notable decrease in kappa number and increased brightness as compared to xylanase alone. Analysis of bleaching conditions had denoted that 8 IU g(-1) of mixed enzyme preparation (xylanase/laccase, 22: 1) had led into maximal removal of lignin from pulp when bleaching was performed at 10% pulp consistency (55 degrees C, pH 9.0) for 3 h. An overall improvement of 21%, 8%, 3%, and 5% respectively in kappa number, brightness, yellowness, and viscosity of pulp was achieved under derived bleaching conditions. Process of enzymatic bleaching was further ascertained by analyzing the changes occurring in polysaccharide and lignin by HPLC and FTIR. The UV absorption spectrum of the compounds released during enzymatic treatment had denoted a characteristic peak at 280 nm, indicating the presence of lignin in released coloring matter. The changes in fiber morphology following enzymatic delignification were studied by scanning electron microscopy.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available