Article
Engineering, Environmental
Natalia A. Rodrigues, Eric T. Katayama, Fernanda P. Casciatori
Summary: This study evaluated alternative modes of operation for a multilayer packed-bed bioreactor (PBB) in solid-state cultivation (SSC) processes, and found that continuous operation is feasible and advantageous, contributing to the operational versatility and scale-up of PBBs in the industrial advancement of SSC.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Biotechnology & Applied Microbiology
Caroline Lopes Perez, Fernanda Perpetua Casciatori, Joao Claudio Thomeo
Summary: The research focused on improving enzyme production through solid-state cultivation using a mixture of sugarcane bagasse and wheat bran to grow Myceliophthora thermophila I-1D3b fungus. Results showed higher enzyme activities in the most porous medium, and using an inner tube for air supply in the pilot-scale bioreactor resulted in more homogeneous and active enzyme production. These findings will be beneficial for scaling up this bioreactor for industrial applications.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Engineering, Chemical
Joao Paulo Henrique, Fernanda Perpetua Casciatori, Joao Claudio Thomeo
Summary: In this study, gas-sampling procedures were compared for sampling respiration gases in a packed-bed bioreactor used for solid-state cultivation. The results showed that sampling gases at low flow rate produced similar results to the end-to-end operation, while inefficient aeration of part of the bed strongly affected the gas concentrations and temperature. The findings provide valuable insights into microbial respiratory activity.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Biotechnology & Applied Microbiology
Ganesh Swain, R. K. Sonwani, R. S. Singh, Ravi P. Jaiswal, B. N. Rai
Summary: In this study, a sponge-modified carrier, polypropylene-polyurethane foam (PP-PUF), was used in MBBR and PBBR for the biodegradation of 4-CP. Under identical conditions, MBBR showed higher removal efficiencies for 4-CP and COD due to the higher growth of biomass.
ENVIRONMENTAL TECHNOLOGY & INNOVATION
(2021)
Article
Engineering, Environmental
Anelize Terezinha Jung Finkler, Mariana Zanlorenzi Weber, Gustavo Alexandre Fuchs, Leandro Aluisio Scholz, Luiz Fernando de Lima Luz, Nadia Krieger, David Alexander Mitchell, Luiz Mario de Matos Jorge
Summary: Although mathematical models of heat and mass transfer in packed-bed bioreactors have been developed for decades, experimental determination of heat and mass transfer coefficients between solids and air phases has been limited. In this study, experiments were conducted in a pilot packed-bed bioreactor to investigate the heat and mass transfer coefficients, which were found to have a lesser effect on temperature profiles compared to varying air flow rates. It was concluded that separate phase models recognizing the non-equilibrium conditions between solids and air are essential for accurate description of the system.
CHEMICAL ENGINEERING JOURNAL
(2021)
Article
Biotechnology & Applied Microbiology
Anelize Terezinha Jung Finkler, Luiz Fernando de Lima Luz Jr, Nadia Krieger, David Alexander Mitchell, Luiz Mario Jorge
Summary: Model-based strategies for scaling up traditional packed-bed bioreactors used in solid-state fermentation have been available for over two decades. However, these strategies are theoretical and not based on experimental data. A key strategy identified in the study is to decrease the temperature of the inlet air during periods of peak heat generation to optimize the performance of large-scale traditional packed-bed bioreactors.
BIOCHEMICAL ENGINEERING JOURNAL
(2021)
Article
Microbiology
Anna Detman, Daniel Laubitz, Aleksandra Chojnacka, Ewa Wiktorowska-Sowa, Jan Piotrowski, Agnieszka Salamon, Wiktor Kazmierczak, Mieczyslaw K. Blaszczyk, Albert Barberan, Yongjian Chen, Ewa Lupikasza, Fei Yang, Anna Sikora
Summary: This study investigates the dynamics and stability of microbial communities producing hydrogen from sugar-beet molasses, highlighting the importance of the balance between hydrogen producers and lactic acid bacteria in dark fermentation for efficient hydrogen production.
FRONTIERS IN MICROBIOLOGY
(2021)
Review
Engineering, Chemical
David Alexander Mitchell, Hector A. Ruiz, Nadia Krieger
Summary: Packed-bed bioreactors are commonly used for aerobic solid-state fermentation in biorefineries. Studies have shown issues with low air velocities, preferential airflow, and ineffective strategies for airflow direction. Furthermore, narrow water-jacketed bioreactors do not accurately reflect heat removal in wide large-scale packed beds. More research is needed to characterize the properties of different solid substrates and their changes due to microbial growth.
Article
Biochemistry & Molecular Biology
Antonio Martinez-Ruiz, Luz Tovar-Castro, Cristobal N. Aguilar, Gerardo Saucedo-Castaneda, Ernesto Favela-Torres
Summary: The invertase produced from Aspergillus niger C28B25 through solid-state fermentation demonstrated high hydrolysis efficiency and stability under different operational conditions, making it a promising biocatalyst for batch or continuous sucrose hydrolysis processes.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2022)
Article
Biotechnology & Applied Microbiology
Maral Tsevelkhoroloo, Vijayalakshmi Dhakshnamoorthy, Young-Soo Hong, Chang-Ro Lee, Soon-Kwang Hong
Summary: In this study, two α-neoagarooligosaccharide hydrolases (SCO3481 and SCO3479) were identified in Streptomyces coelicolor A3(2), which catalyzed the hydrolysis of α-(1,3) glycosidic bonds. SCO3481 belonged to the GH117 family and formed dimers, while SCO3479 was a large monomeric α-neoagarooligosaccharide hydrolase belonging to the GH2 family with β-galactosidase activity. SCO3479, a unique bifunctional enzyme, has many advantages for industrial applications.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2023)
Article
Engineering, Chemical
Karol Dudek, Idania Valdez-Vazquez, Joerg Koop
Summary: The present study investigated the feasibility of vacuum distillation in a rotating packed bed (RPB) for the separation and recovery of butanol, ethanol, and furfural. Further purification is needed to obtain a pure product.
SEPARATION AND PURIFICATION TECHNOLOGY
(2022)
Article
Green & Sustainable Science & Technology
Sasan Zarei, Seyyed Mohammad Mousavi, Teimour Amani, Mehrdad Khamforoush, Arezou Jafari
Summary: This study simulated a lab-scale continuous packed-bed bioreactor with multi-sized distributed particles using computational fluid dynamics. The results showed good agreement with experimental data, with a relative error of less than 15%, making the proposed model suitable for scale-up, optimization, and evaluation of other kinetic models.
Article
Engineering, Environmental
Wen-Hsing Chen, Yun-Ting Luo, Jih-Gaw Lin, Yu-Tzu Huang
Summary: This study investigated the microbial community and energy requirements of an anaerobic packed bed reactor treating low-strength synthetic wastewater under different HRTs and temperatures. The results showed that as the HRT and temperature decreased, COD removal and methane yield also decreased. The microbial community with low relative abundances of archaea and facultative bacteria played a significant role in the reactor's efficiency.
PROCESS SAFETY AND ENVIRONMENTAL PROTECTION
(2022)
Article
Biotechnology & Applied Microbiology
Tumisi Beiri Jeremiah Molelekoa, Thierry Regnier, Laura Suzanne da Silva, Wilma Augustyn
Summary: This study investigated the production of natural pigments by five filamentous fungi isolated from soil and cultured on a mixture of green waste and whey. Optimal conditions for pigment production were found to be at pH 7.0 and 9.0, with higher biomass and pigment intensities observed in solid-state fermentation compared to submerged fermentation. The study suggests that solid-state fermentation may be a feasible strategy for scaling up pigment production in manufacturing industries.
FERMENTATION-BASEL
(2021)
Article
Biotechnology & Applied Microbiology
Nor Syafirah Zambry, Norazurin Syuhada Rusly, Mohd Syafiq Awang, Nur Asshifa Md Noh, Ahmad Ramli Mohd Yahya
Summary: The study focused on lipopeptide biosurfactant production by Streptomyces sp. PBD-410L in batch and fed-batch fermentation using palm oil as the sole carbon source. Optimization of aeration rate and agitation speed in batch cultivation led to an increase in biomass growth and biosurfactant production. Fed-batch cultivation with DO-stat feeding strategy further improved biosurfactant production from 3.74 to 5.32 g/L compared to batch cultivation.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2021)
Article
Plant Sciences
Aswathy Udayan, Ashutosh Kumar Pandey, Ranjna Sirohi, Nidhin Sreekumar, Byoung-In Sang, Sung Jun Sim, Sang Hyoun Kim, Ashok Pandey
Summary: In the current global scenario, the world is facing challenges due to population growth, industrialization, and urbanization. This has led to an increased demand for nutrients and renewable energy sources. Microalgae are considered a promising candidate for high-value metabolites and alternative energy sources. However, current cultivation systems for microalgae do not offer feasibility in terms of technology, economics, energy, and environmental sustainability.
PHYTOCHEMISTRY REVIEWS
(2023)
Article
Plant Sciences
Anil Kumar Patel, Akash Pralhad Vadrale, Reeta Rani Singhania, Philippe Michaud, Ashok Pandey, Shu-Jen Chen, Chiu-Wen Chen, Cheng-Di Dong
Summary: In recent years, algal polysaccharides have been extensively studied for pharmaceutical, nutraceutical, and biomedical applications due to their promising bioactive properties. They are also increasingly being used in industrial and dietary sectors. The marine algae contain a variety of polysaccharides that have a significant impact on the global economy. In the pharmaceutical and biomedicine industries, alginates, fucoidans, ulvans, carrageenans, and chitin are becoming increasingly important.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Biochemistry & Molecular Biology
Grace Sathyanesan Anisha
Summary: alpha-Galactosidases have diverse therapeutic applications in human medicine and are also used in various industrial sectors. Research efforts aim to identify novel alpha-galactosidases with improved catalytic efficiency.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Review
Environmental Sciences
Luong N. Nguyen, Minh T. Vu, Hang P. Vu, Md. Abu Hasan Johir, Leen Labeeuw, Peter J. Ralph, T. M. I. Mahlia, Ashok Pandey, Ranjna Sirohi, Long D. Nghiem
Summary: This paper reviews the current state of microalgal culture development for carbon capture and utilization (CCU) and highlights its potential contribution to addressing climate change challenges. Innovative system designs are crucial to achieve large-scale CO2 capture by microalgae, and downstream processes of microalgal culture are important for the overall success of microalgae-based CCU.
CRITICAL REVIEWS IN ENVIRONMENTAL SCIENCE AND TECHNOLOGY
(2023)
Article
Environmental Sciences
Reshmy Rajasekharan, Arun Karthika Bahuleyan, Aravind Madhavan, Eapen Philip, Raveendran Sindhu, Parameswaran Binod, Mukesh Kumar Awasthi, Ashok Pandey
Summary: This study investigates the utilization of jackfruit peel as a source for natural and fully biodegradable nanocellulose, which is used in the production of bioplastics with Azadirachta indica extracts and polyethylene glycol for antibacterial properties. The findings reveal that the developed bioplastics have favorable physicochemical properties and biodegradability, and they exhibit strong antibacterial activity against food spoilage bacteria and prevent biofilm formation. These bioplastics can be considered as suitable substitutes for food packaging materials and wound dressings.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Plant Sciences
Anh Tuan Hoang, Ranjna Sirohi, Ashok Pandey, Sandro Nizetic, Su Shiung Lam, Wei-Hsin Chen, Rafael Luque, Sabu Thomas, Muslum Arici, Van Viet Pham
Summary: This paper presents recent advances and potential advantages of microalgae in biofuel production, as well as their role in the energy and environmental sectors. The challenges related to cultivation, harvesting techniques, and downstream processes are discussed, along with promising solutions for industrial-scale production. Additionally, the paper analyzes the role of microalgae in the circular economy.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Plant Sciences
Sabeela Beevi Ummalyma, Ranjna Sirohi, Aswathy Udayan, Pooja Yadav, Abhay Raj, Sang Jun Sim, Ashok Pandey
Summary: Microalgae are cell factories that can be used to produce renewable energy, food, feed, pharmaceuticals, and nutraceuticals. The challenges of mass cultivation and biomass recovery limit the industrial application of microalgae. Cultivating microalgae in wastewater has been encouraged for sustainable bioeconomy. Wastewater from the food industry provides a less-toxic growth medium for low-cost microalgal biomass production. Microalgae are potential biocatalysts for wastewater bioremediation and offer a universal alternative for resource recovery from wastewater.
PHYTOCHEMISTRY REVIEWS
(2023)
Review
Environmental Sciences
Vivek Kumar Gaur, Ranjna Sirohi, Mohd Ishfaq Bhat, Krishna Gautam, Poonam Sharma, Janmejai Kumar Srivastava, Ashok Pandey
Summary: The recent studies have shown that micro/nano plastics and antimicrobial resistance genes have harmful effects on the environment and human health. However, there is a lack of research on the interactions between these two factors and their combined influence. The interaction of microplastics has led to the formation of new plastics such as plastiglomerates, pyroplastics, and anthropoquinas. Additionally, the occurrence of microplastics has been found to contribute to the emergence of antimicrobial resistance genes and other pollutants such as polyaromatic hydrocarbons and pesticides. The excessive use of antibiotics following the Covid outbreak has further exacerbated the detrimental effects on human health. This study highlights the relationship between microplastics and antibiotic resistance generation, evaluates the factors of uncontrolled antibiotic use and negligent plastic consumption, and provides future research prospects to accurately assess the serious environmental occurrence of these pollutants.
Review
Environmental Sciences
Cheng-Di Dong, Ashutosh Tiwari, Grace Sathyanesan Anisha, Chiu-Wen Chen, Anusuiya Singh, Dibyajyoti Haldar, Anil Kumar Patel, Reeta Rani Singhania
Summary: In the process of urbanization, anthropogenic activities, industrialization, and economic growth have led to waste management problems, disposal issues, and the emergence of pollutants. To maintain a sustainable environment, bioremediation using microorganisms or enzymes is a promising solution. Laccase, an oxidoreductase enzyme produced by microorganisms, has shown great potential in degrading chemical pollutants. However, challenges such as immobilization and engineering of robust laccase need to be addressed for large-scale application in waste treatment and emerging pollutant degradation.
ENVIRONMENTAL POLLUTION
(2023)
Article
Chemistry, Multidisciplinary
Hoang Anh Tuan, Ashok Pandey, Chen Wei-Hsin, Shams Forruque Ahmed, Sandro Nizetic, Kim Hoong Ng, Zafar Said, Duong Xuan Quang, Umit Agbulut, Hady Hadiyanto, Nguyen Xuan Phuong
Summary: Hydrogen energy is considered an attractive alternative to fossil fuels due to its environmental friendliness. Photocatalysis-derived hydrogen from water splitting is believed to be the optimal solution for meeting long-term sustainability and increased energy demands. Metal and carbon-supported photocatalysts show great potential for solar-driven hydrogen production from water. This review discusses the important aspects of different photocatalytic genres and provides new directions for more advanced performance.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2023)
Review
Agricultural Engineering
Dibyajyoti Haldar, Neelanjan Bhattacharjee, Asma Musfira Shabbirahmed, Grace Sathyanesan Anisha, Anil Kumar Patel, Jo-Shu Chang, Cheng-Di Dong, Reeta Rani Singhania
Summary: This article summarizes recent research on the purification of biogas, discussing methods to improve its quality and reduce CO2 emissions, as well as the use of sustainable materials for gas purification. The article also reviews different gas purification technologies such as water scrubbing, adsorption, cryogenic separation, and biological upgrading, and summarizes numerical models used for predicting biogas upgrading. Finally, the article highlights existing bottlenecks and recommends further research directions.
BIOMASS & BIOENERGY
(2023)
Article
Veterinary Sciences
Tessy Augustianath, D. A. Evans, G. S. Anisha
Summary: The study aimed to investigate the effects of RF-EMR on the embryonic development of organisms using chick embryos as a model. Fertilized eggs were incubated and exposed to RF-EMR from 2G and 4G mobile phones. The results showed that RF-EMR caused teratogenic changes in the morphology of chick embryos, including cross beak, non-retracted yolk sac, macrocephaly, malformed legs and toes, disability in standing and balancing, and variations in body weight, body length, and beak length.
RESEARCH IN VETERINARY SCIENCE
(2023)
Review
Biotechnology & Applied Microbiology
Pitchurajan Krishna Perumal, Cheng-Di Dong, Ajeet Singh Chauhan, Grace Sathyanesan Anisha, Mohammad Sibtain Kadri, Chiu-Wen Chen, Reeta Rani Singhania, Anil Kumar Patel
Summary: In recent years, algal-derived glycans and oligosaccharides have gained importance in health applications due to their higher bioactivities compared to plant-derived oligosaccharides. Marine organisms have complex and highly branched glycans with more reactive groups, which lead to greater bioactivities. However, their complex and large molecules limit their broad commercial applications due to dissolution limitations. On the other hand, oligosaccharides have better solubility and retain their bioactivities, offering better opportunities for applications. Efforts are being made to develop a cost-effective method for enzymatic extraction of oligosaccharides from algal polysaccharides and biomass, and detailed structural characterization of algal derived glycans is required for improved bioactivity and commercial applications. Some macroalgae and microalgae are being evaluated as in vivo biofactories in clinical trials, providing insights into therapeutic responses. This review discusses recent advancements, bottlenecks, technological limitations, and potential solutions in the production of oligosaccharides from microalgae. It also highlights the emerging bioactivities of algal oligosaccharides and their promising potential for biotherapeutic applications.
BIOTECHNOLOGY ADVANCES
(2023)
Article
Energy & Fuels
Anh Tuan Le, Ashok Pandey, Ranjan Sirohi, Prabhakar Sharma, Wei-Hsin Chen, Nguyen Dang Khoa Pham, Viet Dung Tran, Xuan Phuong Nguyen, Anh Tuan Hoang
Summary: Biochar has applications in energy and environmental fields. To predict its characteristics, a hybrid technique combining machine learning algorithms and game theory-based SHAP analysis was developed. The BRT-based model showed the best prediction performance and SHAP analysis revealed temperature as the main factor affecting the predictions. This hybrid technique provides valuable insights for improving control and application of biochar.
Article
Biology
Neha Kumari, Abha Pandey, Amit Gupta, Sonal Mishra, Rajeshwar P. Sinha
Summary: The investigation revealed that scytonemin, a photoprotective compound found in cyanobacteria, helps these organisms survive and thrive in extreme environments by counteracting the harmful effects of solar radiation. This compound is considered a key ingredient for the development of novel drugs and natural sun protection products.
