Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 80, Issue 16, Pages 4788-4794Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.00885-14
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Funding
- NASA Astrobiology: Exobiology and Evolutionary Biology program [NNX08AO15G]
- NASA Planetary Biology Internship (PBI) program
- NASA Earth and Space Science Fellowship (NESSF) program [13-PLANET13F-0084]
- NASA [95807, NNX08AO15G] Funding Source: Federal RePORTER
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Studies of how microorganisms respond to pressure have been limited mostly to the extreme high pressures of the deep sea (i.e., the piezosphere). In contrast, despite the fact that the growth of most bacteria is inhibited at pressures below similar to 2.5 kPa, little is known of microbial responses to low pressure (LP). To study the global LP response, we performed transcription microarrays on Bacillus subtilis cells grown under normal atmospheric pressure (similar to 101 kPa) and a nearly inhibitory LP (5 kPa), equivalent to the pressure found at an altitude of similar to 20 km. Microarray analysis revealed altered levels of 363 transcripts belonging to several global regulons (AbrB, CcpA, CodY, Fur, IolR, ResD, Rok, SigH, Spo0A). Notably, the highest number of upregulated genes, 86, belonged to the SigB-mediated general stress response (GSR) regulon. Upregulation of the GSR by LP was confirmed by monitoring the expression of the SigB-dependent ctc-lacZ reporter fusion. Measuring transcriptome changes resulting from exposure of bacterial cells to LP reveals insights into cellular processes that may respond to LP exposure.
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