Article
Biotechnology & Applied Microbiology
Guo-Teng Liang, Cuixin Lai, Zejun Yue, Hanbin Zhang, Danyang Li, Zhong Chen, Xingyu Lu, Liang Tao, Fedor V. Subach, Kiryl D. Piatkevich
Summary: In this study, a pair of engineered flavin-binding fluorescent proteins, miniGFP1 and miniGFP2, were developed. These miniGFPs showed improved photochemical properties and high sensitivity to transition metal ions, making them potential fluorescent biosensors for live cell imaging and metal ion detection.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biophysics
Hyeran Kim, Seul Gee Hwang, Kyeonghye Guk, Yoonji Bae, Hwangseo Park, Eun-Kyung Lim, Taejoon Kang, Juyeon Jung
Summary: RSV infections can cause severe diseases, and the emergence of drug-resistant RSV strains is concerning. We studied an antibody that can specifically detect palivizumab-resistant RSV strains and developed a rapid detection method for the S275F RSV antigen.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Multidisciplinary
Mengying Deng, Jing Yuan, Haibin Yang, Xuli Wu, Xiaoyuan Wei, Yang Du, Garry Wong, Yuyong Tao, Gang Liu, Zongwen Jin, Jun Chu
Summary: This study introduced a bright green fluorescent protein, Clover4, obtained through structure-guided mutagenesis, which showed improved performance in LUMABS sensors for antibody detection in serum. The Clover4-LUMABS sensors exhibited a larger dynamic range and lower limit of detection compared to previous sensors, offering a promising approach for fast and highly sensitive antibody detection.
Article
Chemistry, Physical
Arusha Acharyya, Wenkai Zhang, Feng Gai
Summary: Most biological systems are complex, diverse, and non-fluorescent, requiring the development of diverse fluorescence reporters for studying their structures, dynamics, and interactions without affecting their native properties. Tryptophan-based fluorescent amino acids have been identified as a potential solution for this need, with recent progress and future directions towards their development.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Veterinary Sciences
Dong-Kun Yang, Ha-Hyun Kim, Yu-Ri Park, Jae Young Yoo, Yeseul Park, Jungwon Park, Bang-Hun Hyun
Summary: The study aimed to improve the traditional FAVN method by constructing a new recombinant RABV expressing green fluorescent protein (GFP). The research confirmed the stability and immunogenicity of the new virus expressing GFP in VERO cells and in mice, suggesting its potential as a safer alternative for the FAVN test.
JOURNAL OF VETERINARY SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Anna Miriam John, Harsimranjit Sekhon, Jeung-Hoi Ha, Stewart N. Loh
Summary: Protein conformational switches are widely used in biosensing to couple input and output signals. This study creates a biosensor using a binding-induced folding mechanism to drive a conformational shift, resulting in fluorescence changes. The design has the advantages of genetic encodability and modularity.
Article
Agriculture, Multidisciplinary
Shumao Cui, Cailing Chen, Jiayu Gu, Bingyong Mao, Hao Zhang, Jianxin Zhao, Wei Chen
Summary: The study genetically engineered Lactobacillus plantarum ST-III to express GFP and studied its distribution in the intestine of mice using fluorescence imaging. The results showed that the genetically modified strain could survive in the intestine and be detected at various time points. The fluorescence intensity was found to have a linear relationship with the viable count of the strain, suggesting that fluorescence intensity can be used to estimate the amount of the strain in the intestinal tract.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Article
Biochemistry & Molecular Biology
Lidia Ruiz-Llorente, M. Cristina Vega, Francisco J. Fernandez, Carmen Langa, Nicholas W. Morrell, Paul D. Upton, Carmelo Bernabeu
Summary: sEng is the soluble form of endoglin, expressed at high levels in various diseases. It serves as a marker of cardiovascular damage and tumor development, plays a pathogenic role in conditions like preeclampsia, and inhibits angiogenesis and tumor proliferation, migration, and invasion.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Food Science & Technology
Xinqi Sun, Jing Zhang, Qian Lu, Jiao Tian, Dandan Kong, Jiaoyang Luo, Meihua Yang
Summary: A novel labeled antibody was synthesized using fluorescent nanoparticles, and a fluorescence immunoassay method was established for the detection of fipronil in edible flowers. The method showed high sensitivity with detection limits of 0.21 μg/L and 8.61 μg/L, and was 3.5 times more sensitive than the enzyme-linked immunosorbent assay. The developed method was successfully applied for the determination of fipronil levels in edible flower samples and validated with gas chromatography-tandem mass spectrometry.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Physical
Adrian Chiong Yuh Tiong, Inn Shi Tan, Henry Chee Yew Foo, Man Kee Lam, Hisham Ben Mahmud, Keat Teong Lee
Summary: The synthesis of Janus nanosheets using kappa-carrageenan as a green template provides a greener and more straightforward method compared to traditional approaches. By effectively masking one facet of graphene oxide through hydrogen bonding interactions, an asymmetrically modified graphene oxide is produced. This newly developed method offers sustainability and flexibility in material synthesis protocols.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Optics
Cheng Fu, Dongdong Zhang, Gongnv Xu, Xuankai Deng, Min Liu, Yun Deng, Wangting Lu, Chaohui Chen, Yibin Ruan, Yanhua Yu
Summary: This study designed and synthesized two fluorescent molecules, m-DBI and p-DBI, with m-DBI showing strong fluorescence in aprotic organic solvents and forming microtubes with FF, while p-DBI exhibited weak fluorescence in both protic and aprotic solvents and could not co-assemble with FF. This research demonstrates the potential application of screening possible inhibitor molecules of AD diseases using FF-m-DBI co-assemblies.
JOURNAL OF LUMINESCENCE
(2021)
Review
Biochemistry & Molecular Biology
Yu-Lei Chen, Xin-Xin Xie, Ning Zhong, Le-Chang Sun, Duanquan Lin, Ling-Jing Zhang, Ling Weng, Tengchuan Jin, Min-Jie Cao
Summary: Since the discovery of fluorescent proteins, their unique properties have been widely used in biological research. Antibodies targeting these proteins, including nanobodies, have emerged and shown great potential. Nanobodies are a new type of antibody that are small, stable, and functional in living cells, and they can easily access hidden antigenic epitopes on the target surface. This review provides an overview of various fluorescent proteins, their antibodies, especially nanobodies, and advanced applications of nanobodies targeting fluorescent proteins. It is a valuable resource for further research in this field and enhances the value of fluorescent proteins in biological research.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Taylor D. Krueger, Longteng Tang, Chong Fang
Summary: Fluorescent proteins (FPs) are important tools for noninvasive bioimaging and sensing. REX-GECO1, a red-green emission and excitation ratiometric FP-based biosensor, shows a high dynamic range in measuring cellular calcium concentrations. The potential energy surfaces of these biosensors are revealed using electronic and spectroscopic techniques, uncovering details of their behavior. These findings can inform the design of biosensors with improved efficiency for calcium imaging.
Article
Biotechnology & Applied Microbiology
J-Pablo Salvador, Thomas Brettschneider, Christian Dorrer, M-Pilar Marco
Summary: This study developed a point-of-care device for the detection of oral anticoagulants. The device is automatic, rapid, sensitive, and disposable, and can assist clinicians in monitoring patients under oral anticoagulant treatment. The study demonstrated that the device has good reproducibility and accuracy.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Multidisciplinary Sciences
Zhiyu Qu, Jing Fang, Yu-Xiang Wang, Yibin Sun, Yajie Liu, Wen-Hao Wu, Wen-Bin Zhang
Summary: A single-domain green fluorescent protein catenane was designed and synthesized by rewiring the connectivity of GFP's secondary motifs, showcasing the rich structural diversity of proteins. This design and synthesis strategy can be applied to other proteins with similar folds, expanding the family of single-domain fluorescent proteins.
NATURE COMMUNICATIONS
(2023)