Journal
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
Volume 78, Issue 23, Pages 8467-8469Publisher
AMER SOC MICROBIOLOGY
DOI: 10.1128/AEM.02288-12
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Funding
- Institut Pasteur
- French Ministry of Research [ANR-08-MIE-018]
- University Paris Diderot
- USPHS [AI29743, DE04645]
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We have developed a capillary tube assay in combination with real-time PCR to quantitate the number of chemoattracted Leptospira cells. We identified Tween 80, glucose, sucrose, and pyruvate as attractants for Leptospira cells; amino acids and vitamin B-12 were found to be nonchemotactic or weakly chemotactic. This assay has the general applicability to further our understanding of leptospiral chemotaxis.
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